, Volume 8, Issue 4, pp 337–342 | Cite as

Direct modification of plasma low-density lipoproteins in interstitial inflammatory fluid of the rabbit

  • Thomas L. Raymond
  • Scott A. Reynolds
Original Articles


Utilizing the polyvinyl sponge-implant model in the rabbit, we have previously demonstrated modification in low-density lipoproteins (LDL) in the extravascular space in association with a cellular inflammatory response. In an attempt to isolate the source of these modifications, plasma LDL was labeled with125I, and introduced directly into the extravascular space at the time of sponge implantation. [125I] plasma LDL recovered from interstitial inflammatory fluid (IF) at 24 h after implantation demonstrated increased electrophoretic mobility as well as heterogeneity in particle size and hydrated density. These results are in agreement with our previous observations and indicate that modification in IF-LDL probably occurs after it has entered the extravascular space across the vascular wall.


Public Health Internal Medicine Hydrated Inflammatory Response Particle Size 
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  1. 1.
    Yoffey J. M., andF. C. Courtice. 1970. Lymphatics, Lymph and the Lymphomyeloid Complex. Academic Press, London.Google Scholar
  2. 2.
    Reichl D. L., L. A. Simons, N. B. Myant, J. J. Pflug, andG. L. Mills. 1973. The lipids and lipoproteins of human peripheral lymph, with observations on the transport of cholesterol from plasma and tissue into lymph.Clin. Sci. Mol. Med. 45:313–329.Google Scholar
  3. 3.
    Raymond T. L., andS. A. Reynolds. 1983. Lipoproteins of the extravascular space: Alterations in low density lipoproteins of interstitial inflammatory fluid.J. Lipid Res. 24:113–119.Google Scholar
  4. 4.
    Henriksen T., E. M. Mahoney, andD. Steinberg. 1981. Enhanced macrophage degradation of low density lipoproteins previously incubated with cultured endothelial cells: Recognition by receptors for acetylated low density lipoproteins.Proc. Natl. Acad. Sci. U.S.A. 77:2214–2218.Google Scholar
  5. 5.
    Hoff H. F., andJ. W. Gaubatz. 1982. Isolation, purification and characterization of a lipoprotein containing apoB from the human aorta.Atherosclerosis 42:273–279.Google Scholar
  6. 6.
    Henriksen T., E. M. Mahoney, andD. Steinberg. 1983. Enhanced macrophage degradation of biologically modified low density lipoprotein.Arteriosclerosis 3:149–159.Google Scholar
  7. 7.
    Raymond T. L., D. S.Leake, and D.Steinberg. Unpublished observations.Google Scholar
  8. 8.
    Havel R. J., H. A. Eder, J. H. Bragdon, 1955. The distribution and chemical composition of ultracentrifugally separated lipoproteins in human serum.J. Clin. Invest. 34:1345–1353.Google Scholar
  9. 9.
    Fraker P. J., andJ. C. Speck. 1978. Proteinand cell membrane iodinations with a sparingly soluble chloramine, 1,3,4,6-tetrachloro-3a, 6a-diphenylgiycolunl.Biochem. Biophys, Res. Commun. 80:849–857.Google Scholar
  10. 10.
    Weiner S. L., R. Havier, M. Urivetsky, R. J. Friedman, T. H. Spaet, B. W. Arbogast, andT. L. Raymond. 1981. Inflammatory fluid, plasma and serum growth factor activity for early passage fibroblasts. The effect of leukopenia and thrombocytopenia in polyvinyl sponge implanted rabbits.Connect. Tissus Res. 9:33–50.Google Scholar
  11. 11.
    Noble R. P., 1968. Electrophoretic separation of plasma lipoproteins in agarose gel.J. Lipid Res. 9:693–700.Google Scholar
  12. 12.
    Goldstein J. L., Y. K. Ho, S. K. Basu, andM. S. Brown. 1979. Binding site on macrophages that mediates uptake and degradation of acetylated low density lipoprotein, producing massive cholesterol deposition.Proc. Natl. Acad. Sci. U.S.A. 76:333–337.Google Scholar
  13. 13.
    Raymond T. L., andS. A. Reynolds. 1983. Lipoprotein metabolism in the extravascular space: Cyclophosphamide inhibits modification of low density lipoproteins in interstitial inflammatory fluid of the rabbit.Inflammation 7:241–246.Google Scholar

Copyright information

© Plenum Publishing Corporation 1984

Authors and Affiliations

  • Thomas L. Raymond
    • 1
  • Scott A. Reynolds
    • 1
  1. 1.Department of Internal MedicineEast Tennessee State UniversityJohnson City

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