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Isolation and characterization of a noncytotoxic mast-cell activator from cobra venom

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Abstract

A protein has been isolated and partially purified from the venom ofNaja naja which causes the noncytolytic release of amines from rat peritoneal mast cells. This protein, termed CVA protein, has been demonstrated to have a molecular weight of 18,500 and sedimentation coefficient of approximately 2s. The activity of CVA protein is destroyed by treatment for 5 min at 100°C, but is not affected at 75°C for 30 min. The ability of CVA protein to initiate mast-cell degranulation has been demonstrated to require both cell energy and calcium. The temperature sensitivity and the cellular requirements distinguish the CVA protein from phospholipase A.

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Authors and Affiliations

  1. Department of Immunopathology, Scripps Clinic and Research Foundation, La Jolla, California

    David C. Morrison, Janet F. Roser, Peter M. Henson & Charles G. Cochrane

Authors
  1. David C. Morrison
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  2. Janet F. Roser
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  3. Peter M. Henson
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  4. Charles G. Cochrane
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Additional information

This work was supported by Grant AI-07007, the American Heart Association, the Council for Tobacco Research, and GMS Grant GM 19322-05

Recipient of U.S. Public Health Service Career Development Award GM 42567-05.

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Morrison, D.C., Roser, J.F., Henson, P.M. et al. Isolation and characterization of a noncytotoxic mast-cell activator from cobra venom. Inflammation 1, 103–115 (1975). https://doi.org/10.1007/BF00918063

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