, Volume 1, Issue 1, pp 85–91 | Cite as

Ultrastructural alterations during ATP-induced secretion of lysosomal enzymes from rabbit polymorphonuclear leukocytes

  • P. M. Henson
  • J. E. Henson
  • E. L. Becker


Rabbit neutrophils incubated in low-ionic-strength media were stimulated by ATP to secrete lysosomal enzymes. This was greatly enhanced in the presence of cytochalasin B. ATP in these circumstances induced the cell to form large cytoplasmic extensions that were largely devoid of granules. In the presence of both ATP and cytochalasin B, however, the projections contained granules in close proximity to the cell membrane. Neutrophils in low-ionic-strength buffer were capable of binding to zymosan particles coated with C3b but not of phagocytizing them. Release of granule enzymes was observed and exocytosis of granules appeared to occur at sites distant from those portions of the plasma membrane adherent to the particle.


Public Health Cell Membrane Internal Medicine Cytochalasin Lysosomal Enzyme 
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  1. 1.
    Henson, P.M. 1971. The immunologic release of constituents from neutrophil leukocytes. I. The role of antibody and complement on nonphagocytosable surfaces or phagocytosable particles.J. Immunol. 107:1535.Google Scholar
  2. 2.
    Weissmann, G., R.B. Zurier, P.J. Spieler, andI.N. Goldstein. 1971. Mechanisms of lysosomal enzyme release from leukocytes exposed to immune complexes and other particles.J. Exp. Med. 134:149.Google Scholar
  3. 3.
    Hawkins, D. 1971. Biopolymer membrane: A model system for the study of the neutrophilic response to immune complexes.J. Immunol. 107:344.Google Scholar
  4. 4.
    Henson, P.M. 1971. The immunologic release of constituents from neutrophil leukocytes. II. Mechanisms of release during phagocytosis, and adherence to nonphagocytosable surfaces.J. Immunol. 107:1547.Google Scholar
  5. 5.
    Henson, P.M. 1971. Interaction of cells with immune complexes: adherence, release of constituents, and tissue injury.J. Exp. Med. 134:114s.Google Scholar
  6. 6.
    Woodin, A.M., andA.A. Wieneke. 1970. Site of protein secretion and calcium accumulation in the polymorphonuclear leukocyte treated with leucocidin.In Calcium and Cellular Function. A.W. Cuthbent, editor. St. Martin's Press, New York. 183.Google Scholar
  7. 7.
    Hawkins, D. 1972. Neutrophilic leukocytes in immunologic reactions: evidence for the selective release of lysosomal constituents.J. Immunol. 108:310.Google Scholar
  8. 8.
    Becker, E.L., andP.M. Henson. 1973.In vitro studies of immunologically induced secretion of mediators from cells and related phenomena.In Advances in Immunology, V. 17. F.J. Dixon and H.G. Kunkel, editors. Academic Press, New York. 93.Google Scholar
  9. 9.
    Goldstein, I.M., S. Hoffstein, J. Gallin, andG. Weissmann. 1973. Mechanism of lysosomal enzyme release from human leukocytes: microtubule assembly and membrane fusion induced by a component of complement.Proc. Natl. Acad. Sci. 70:2916.Google Scholar
  10. 10.
    Becker, E.L., H.J. Showell, P.M. Henson, andL.S. Hsu. 1974. The ability of chemofactic factors to induce lysosomal enzyme release. I. The characteristics of the release, the importance of surfaces and the relation of enzyme release to chemotactic responsiveness.J. Immunol. 112:2024.Google Scholar
  11. 11.
    Becker, E.L., andP.M. Henson. 1975. Biochemical characteristics of ATP-induced secretion of lysosomal enzymes from rabbit polymorphonuclear leukocytes.Inflammation 1:71.Google Scholar
  12. 12.
    Henson, P.M., andZ.G. Oades. 1973. Enhancement of immunologically induced granule exocytosis from neutrophils by cytochalasin B.J. Immunol. 110:290.Google Scholar
  13. 13.
    Davies, P., R.I. Fox, M. Polyzonis, A.C. Allison, andA.D. Haswell. 1973. The inhibition of phagocytosis and facilitation of exocytosis in rabbit polymorphonuclear leukocytes by cytochalasin B.Lab. Invest. 28:16.Google Scholar
  14. 14.
    Becker, E.L., andH.J. Showell. 1974. The ability of chemotactic factors to induce lysosomal enzyme release. II. The mechanism of release.J. Immunol. 112:2045.Google Scholar
  15. 15.
    Zurier, R.B., G. Weissmann, S. Hoffstein, S. Kammerman, andH.H. Tai. 1974. Mechanisms of lysosomal enzyme release from human leukocytes. II. Effects of cAMP and cGMP, autonomic agonists, and agents which affect microtubule function.J. Clin. Invest. 53:297.Google Scholar
  16. 16.
    Freed, J.J., andM.M. Lebowitz. 1970. The association of a class of saltatory movements with microtubules in cultured cells.J. Cell Biol. 45:334.Google Scholar

Copyright information

© Plenum Publishing Corporation 1975

Authors and Affiliations

  • P. M. Henson
    • 1
    • 2
  • J. E. Henson
    • 1
    • 2
  • E. L. Becker
    • 1
    • 2
  1. 1.Department of ImmunopathologyScripps Clinic and Research FoundationLaJolla
  2. 2.Department of PathologyUniversity of Connecticut Health CenterFarmington

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