Abstract
Lysis of14C-labeledStaph. aureus by human blood leukocyte lysates, by extracts of rabbit small intestines and pancreas, and by the “cocktail” of enzymes (containing trypsin, lysolecithin, and lysozyme) is strongly inhibited by anionic polyelectrolytes (e.g., heparin, chondroitin sulfate, liquoid (polyanethole sulfonic acid), and DNA). Most of the lytic agents employed were inhibited by cationic polyelectrolytes (e.g., histone, protamin sulfate and polylysin), as well as by gold thiomalate, normal human serum, synovial fluids obtained from patients with knee-joint trauma, extracts of coffee, tea, and cocoa, Ultracorten- and Dexamethasone. On the other hand, some antiinflammatory agents tested (e.g., indomethacin, aspirin, hydrocortisone acetate and succinate, and prednisolone acetate and tributyl acetate) were not inhibitory. All the cationic polyelectrolytes employed and liquoid were also strong inhibitors of lysozyme. Since mixtures of cationic and anionic polyelectrolytes at equimolar concentrations failed to inhibit bacteriolysis, it is postulated that the balance between charged macromolecular substances, which are likely to accumulate in inflammatory foci, may determine the fate of cellular components of bacteria in inflamed tissues. The possible role played by lysosomal enzymes and by tissue inhibitors in tissue damage and in the survival of bacteria in chronic inflammatory lesions is discussed.
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This investigation was supported in part by grants from the Central Research Fund of the Hebrew University and from the Joint Research Fund of the Hebrew University Hadassah School of Dental Medicine founded by the Alpha Omega Fraternity and the Hadassah Medical Organization; by grants from the Chief Scientist, The Ministry of Health, Government of Israel; and the Max Bogen Research Fund obtained through the Friends of the Hebrew University in the United States.
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Sela, M.N., Lahav, M., Ne'eman, N. et al. The effect of leukocyte hydrolases on bacteria. Inflammation 1, 57–69 (1975). https://doi.org/10.1007/BF00918059
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DOI: https://doi.org/10.1007/BF00918059