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The effect of leukocyte hydrolases on bacteria

IV. The role played by artificial enzyme “cocktails” and tissue enzymes in bacteriolysis

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Abstract

Acid hydrolases of human blood leukocytes are highly lytic toStaph. albus, Staph. aureus, andStrep. faecalis. On the other hand, group A and viridans streptococci, encapsulated staphylococci, a variety of Gramnegative rods, andMyc. smegmatis are highly resistant to lysis by leukocyte extracts. The lytic effect of the leukocyte extracts can be mimicked by an artificial “cocktail” which contains crude trypsin, lysolecithin, phospholipase C, and lysozyme. This enzyme mixture is lytic to certain Gram-negative bacteria and encapsulated staphylococci which are resistant to lysis by leukocyte enzymes. Both the leukocyte lysates and the artificial cocktail are more lytic to bacteria harvested from the logarithmic phase of growth than to older cells.Staph. albus andStrep. faecalis, which are not lysed to any appreciable extent by extracts of rabbit intestines, lymphocytes, and platelets, undergo extensive lysis upon the addition of lysozyme, indicating that these cells contain preparatory prolytic agents which are activated by lysozyme. On the other hand, the lysis ofStaph. aureus by extracts of all these cells is less dependent upon lysozyme, indicating that other non-lysozyme-dependent lytic factors are involved in the lysis of this microorganism by certain tissue extracts. It is suggested that the resistance to lysis by leukocyte enzymes of bacterial cell-wall constituents may contribute to the pathogenesis of chronic sequellae, and that artificial enzyme cocktails be used for in vivo treatment of certain chronic inflammatory processes induced by bacteria.

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References

  1. Ginsburg, I., M. Lahav, andN. Ne'eman. 1973. The effect of anionic and cationic polyelectrolytes and antibodies on lysis of bacteria by lysozyme and leukocyte lysates.Israel J. Med. Sci. 9:663.

    Google Scholar 

  2. Lahav, M., N. Ne'eman, E. Adler, andI. Ginsburg. 1974. The effect of leukocyte hydrolases on bacteria. I. Degradation of14C-labeled streptococci and staphylococci by leukocyte lysatesin vitro.J. Infect. Dis. 129:528.

    Google Scholar 

  3. Ne'eman, N., M. Lahav, andI. Ginsburg. 1974. The effect of leukocyte hydrolases on bacteria. II. The synergistic action of lysozyme and extracts of PMN, macrophages, lymphocytes and platelets in bacteriolysis.Proc. Soc. Exp. Biol. Med. 146:1137.

    Google Scholar 

  4. Ginsburg, I., N. Ne'eman, R. Gallily, andM. Lahav. 1975. Degradation and survival of bacteria in sites of allergic inflammation.In Infection and Immunity in the Rheumatic Diseases. D.C. Dumonde, editor. Blackwell Scientific Publications, Oxford, England. In press.

    Google Scholar 

  5. Ginsburg, I., M.Lahav, N.Ne'eman, and J. M.James. 1974. The inhibition by basic and acidic polyelectrolytes of the degradation of bacteria by leukocyte enzymes: relation to the persistence of microbial constituents in inflammatory sites.In Activation of Macrophages. Second Workshop Conference Hoechst. H. Wagner, editor. Excerpta Medica Publications. 162

  6. Lahav, M., N. Ne'eman, J. James, andI. Ginsburg. 1975. The effect of leukocyte hydrolases on bacteria. III. Bacteriolysis induced by extracts of different leukocyte populations and the inhibition of lysis by macromolecular substances.J. Infect. Dis. 131:149.

    Google Scholar 

  7. Ohanian, S.H., andJ.H. Schwab. 1967. Persistence of group A streptococcal cell walls related to chronic inflammation of rabbit dermal connective tissue.J. Exp. Med. 125: 1137.

    Google Scholar 

  8. Ginsburg, I. 1972. Mechanisms of cell and tissue injury induced by group A streptococci: Relation to poststreptococcal sequelae.J. Infect. Dis. 126:264(Part I);126:419 (Part II).

    Google Scholar 

  9. Ginsburg, I., S. Mitrani, N. Ne'eman, andM. Lahav. 1975. Granulomata in streptococcal inflammation: mechanisms of localization, transport and degradation of streptococci in inflammatory sites.In Mononuclear Phagocytes in Immunity Infection and Pathology. R. van Furth, editor. Blackwell Scientific Publication Ltd., Oxford, England. Chap. 64, pp. 1–34.

    Google Scholar 

  10. Sela, M., M. Lahav, N. Ne'eman, Z. Duchan, andI. Ginsburg. 1975. The effect of leukocyte hydrolases on bacteria. V. The modification of bacteriolysis by antiinflammatory agents and by cationic and anionic polyelectrolytes.Inflammation 1:57.

    Google Scholar 

  11. Lowry, O.H., J.J.R. Rosenbrough, A.C. Farr, andR.J. Randall. 1951. Protein measurement with Folin phenol reagent.J. Biol. Chem. 193:265.

    Google Scholar 

  12. Coleman, S.E., I. Van De Rijn, andA.J. Bleiweiss. 1971. Lysis of grouped and ungrouped streptococci by lysozyme.Infect. Immunol. 2:563.

    Google Scholar 

  13. Thacore, H., andH.P. Willet. 1963. Formation of spheroplasts ofMycobacterium tuberculosis by lysozyme treatment.Proc. Soc. Exp. Biol. Med. 114:43.

    Google Scholar 

  14. Willet, H.P., andH. Thacore. 1967. The formation of spheroplasts ofMycobacterium tuberculosis by lysozyme in combination with certain enzymes of rabbit peritoneal monocytes.Can. J. Microbiol. 13:481.

    Google Scholar 

  15. Dannenberg, A.M. 1968. Cellular hypersensitivity and cellular immunity in the pathogenesis of tuberculosis: specificity, systemic and local nature and associated macrophage enzymes.Bacteriol. Rev. 32:85.

    Google Scholar 

  16. McGhee, J.R., andB.A. Freeman. 1970. Effect of lysosomal enzymes on Brucellae.J. Reticuloendothel. Soc. 8:208.

    Google Scholar 

  17. Friedberg, D., andM. Shilo. 1970. Interaction of Gram-negative bacteria with the lysosomal fraction of polymorphonuclear leukocytes. I. Role of cell-wall composition ofSalmonella typhymurium.Infect Immunol. 1:305.

    Google Scholar 

  18. Glick, A.B., J.M. Ranhand, andR.M. Cole. 1972. Degradation of group A streptococcal cell walls by egg-white lysozyme and human lysosomal enzymes.Infect. Immunol. 6:403.

    Google Scholar 

  19. Hirsch, J.G. 1956. Phagocytin: a bactericidal substance from polymorphonuclear leukocytes.J. Exp. Med. 103:589.

    Google Scholar 

  20. Zeya, H.I., andJ.K. Spitznagel. 1966. Cationic proteins of polymorphonuclear leukocyte lysosomes. II. Composition, properties and mechanism of antibacterial action.J. Bact. 91:755.

    Google Scholar 

  21. Li, W., andS. Mudd. 1966. Serum effect on the killing ofStaphylococcus aureus by human leukocyte extracts.J. Immunol. 97:41.

    Google Scholar 

  22. Shayegani, M.G. 1968. Action of lysates of leukocytic granules on Staphylococcal cell walls.J. Infect. Dis. 118:402.

    Google Scholar 

  23. Klebanoff, S.J. 1968. Myeloperoxidase-halide-hydrogen peroxide antibacterial system.J. Bacteriol. 95:2131.

    Google Scholar 

  24. Page, R.C., P. Davies, andA.C. Allison. 1974. Participation of mononuclear phagocytes in chronic inflammatory diseases.J. Reticuloendothel. Soc. 15:413.

    Google Scholar 

  25. Feingold, D.S., J.N. Goldman andA.M. Kuritz. 1968. Locus of action of serum and the role of lysozyme in the serum bactericidal reaction.J. Bacteriol. 96:2118.

    Google Scholar 

  26. Glynn, A.A. 1969. The complement lysozyme sequence in immune bacteriolysis.Immunology.16:463.

    Google Scholar 

  27. Ghuysen, J.M. 1968. Use of bacteriolytic enzymes in determination of wall structure and their role in cell metabolism.Bacteriol. Rev. 32:425.

    Google Scholar 

  28. Sadvik, O., andT. Hoyem. 1969. Inhibitory effect of lysolecithin on bacterial growth.Acta Pathol. Microbiol. Scand. 77:283.

    Google Scholar 

  29. Barrett, A.J. 1969. Properties of lysosomal enzymes.In Lysosomes in Biology and Pathology, Vol. 2. J.T. Dingle and H.B. Fell, editors. North Holland Publishing Co. 245.

  30. Thines-Sempoux, D. 1968. Les lipides des lysosomes et d'autres constituants subcellulaires. Thesis, University of London. Cited in P.J. Jacques and L. Demoulin-Brahy. 1974. Lysomotropic Triton WR-1339. One method of applied lysosomology for the therapy of exoplasmic parasitoses.In Activation of Macrophages. Second Workshop Conference Hoechst. H. Wagner, H. Hahn and R. Evans, editors. Excerpta Medica, Amsterdam. 84.

  31. Davis, S.D., I. Antoinette, andR.J. Wedgwood. 1972. Bactericidal reactions in serum.In Biological Activities of Complement, D.E. Ingram (ed). S. Karger, Basel. 43.

    Google Scholar 

  32. Hirsch, J.G. 1958. The bactericidal action of histone.J. Exp. Med. 108:925.

    Google Scholar 

  33. Janoff, A., andJ. Blondin. 1973. The effect of human granulocyte elastase on bacterial suspensions.Lab. Invest. 29:454.

    Google Scholar 

  34. De Duve, C., andA. Trouet. 1973. Lysosomes and lysosomotropic drugs in hostparasite relationship.In Non-Specific Factors Influencing Host Resistance. W. Braun and E. Ungar, editors. S. Karger, Basel. 153.

    Google Scholar 

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Authors and Affiliations

  1. Department of Oral Biology, Faculty of Dental Medicine, Hebrew University, Jerusalem, Israel

    Isaac Ginsburg, Nurit Neeman, Zvia Duchan, Michael N. Sela, Jennifer James & Meir Lahav

  2. Division of Immunology, Clinical Research Centre, Harrow, Middlesex, England

    Isaac Ginsburg, Nurit Neeman, Zvia Duchan, Michael N. Sela, Jennifer James & Meir Lahav

Authors
  1. Isaac Ginsburg
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  2. Nurit Neeman
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  3. Zvia Duchan
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  4. Michael N. Sela
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  5. Jennifer James
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  6. Meir Lahav
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Additional information

This investigation was supported in part by grants from the Central Research Fund of the Hebrew University and from the Joint Research Fund of the Hebrew University Hadassah School of Dental Medicine founded by the Alpha Omega Fraternity and the Hadassah Medical Organization, by grants from the Chief Scientist, the Ministry of Health, Government of Israel and the Max Bogen Research Fund obtained through the Friends of the Hebrew University in the United States.

Part of this work was performed at the Clinical Research Centre, Harrow, Middlesex, England, under a scholarship from the Nuffield Foundation, London.

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Ginsburg, I., Neeman, N., Duchan, Z. et al. The effect of leukocyte hydrolases on bacteria. Inflammation 1, 41–56 (1975). https://doi.org/10.1007/BF00918058

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