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Journal of Clinical Immunology

, Volume 9, Issue 6, pp 437–447 | Cite as

Polymerase chain reaction

  • Henry A. Erlich
Special Article

Summary

The PCR, like recombinant DNA technology, has had an enormous impact in both basic and diagnostic aspects of molecular biology because it can produce large amounts of a specific DNA fragment from small amounts of a complex template. Recombinant DNA techniques create molecular clones by conferring on a specific sequence the ability to replicate by inserting it into a vector and introducing the vector into a host cell. PCR represents a form of “in vitro cloning” that can generate, as well as modify, DNA fragments of defined length and sequence in a simple automated reaction. In addition to its many applications in basic molecular biological research, PCR promises to play a critical role in the identification of medically important sequences as well as an important diagnostic one in their detection.

Key words

Polymerase chain reaction DNA denaturing gradient gel electrophoresis 

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Copyright information

© Plenum Publishing Corporation 1989

Authors and Affiliations

  • Henry A. Erlich
    • 1
  1. 1.Department of Human GeneticsCetus CorporationEmeryville

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