Abstract
Preparations of IgG 3 isolated by absorption of IgG 1, IgG 2, and IgG 4 from a human iv immunoglobulin with protein A-Sepharose were evaluated for their opsonic activities against type III group B streptococcal (GBS) strains. The resulting preparations were free of IgG 1 and IgG 2 and contained only trace amounts of IgG 4 (<2% of total IgG). These IgG 3 preparations exhibited excellent opsonic activities against type III GBS strains, similar to those of the unfractionated iv immunoglobulin (based on total IgG concentrations in the opsonic assays). In contrast, preparations of IgG 1, 2, and 4 eluted from protein A-Sepharose with 2M acetic acid and 7M urea were significantly less effective in enhancing phagocytosis and killing of type III GBS than IgG 3 preparations or iv immunoglobulin. The reasons for excellent opsonic activity of IgG 3 preparations as well as for decreased opsonic activity of IgG 1, 2, and 4 preparations are not clear. Perhaps alteration of IgG by lower pH and high concentrations of urea may have impaired the functional activity of IgG 1, 2, and 4 preparations. The significant finding of this study is the first demonstration of the excellent opsonic activity of IgG 3, emphasizing the importance of having intact IgG 3 in commercial immunoglobulin preparations used in prophylaxis or treatment of GBS infections.
Similar content being viewed by others
References
Kim KS, Wass CA, Kang JH, Anthony BF: Functional activities of various preparations of human intravenous immunoglobulin against type III group B streptococcus. J Infect Dis 153:1092–1097, 1986
Sjoquist J, Meloun B, Hjelm H: Protein A isolated fromStaphylococcus aureus after digestion with lysostaphin. Eur J Biochem 29:572–578, 1972
Kronvall G, Williams RC Jr: Differences in antiprotein A activity among IgG subgroups. J Immunol 103:828–833, 1969
Burton DR, Gregory L, Jefferies R:Aspects of the Molecular Structure of IgG Subclasses, Monographs in Allergy, Vol19, F Shakib (ed). Basel, New York, 1986, pp 7–35
Kim KS, Anthony BF: Penicillin tolerance in group B streptococci isolated from infected neonates. J Infect Dis 144:411–419, 1981
Hjelm H: Isolation of IgG3 from normal human sera and from a patient with multiple myeloma by using protein A-Sepharose 4B. Scand J Immunol 4:633–639, 1975
Lee WY, Heiner DC: Preparation of rabbit anti-IgE for use in radioimmunoassays of total IgG and specific IgE antibodies. J Immunol Methods 20:185–200, 1978
Anthony BF, Concepcion NF, McGeary SA, Ward JI, Heiner DC, Shapshak P, Insel RA: Immunospecificity and quantitation of an enzyme-linked immunoabsorbent assay for group B streptococcal antibody. J Clin Microbiol 350–354, 1982
Colton T:Statistics in Medicine. Boston, Little, Brown, 1974
Hammarstrom L, Granstrom M, Oxelius V, Persson MAA, Smith CIE: IgG subclass distribution of antibodies againstS. aureus teichoic acid and toxin in normal and immunodeficient donors. Clin Exp Immunol 55:593–601, 1984
Rieson WF, Skvaril F, Braun DG: Natural infection of man with group A streptococci. Levels; restriction in class, subclass, and type; and clonal appearance of polysaccharide-group-specific antibodies. Scand J Immunol 383–390, 1976
Siber GR, Schur PH, Aisenberg AC, Weitzman SA, Schiffman G: Correlation between serum IgG-2 concentrations and the antibody response to bacterial polysaccharide antigens. N Engl J Med 303:178–182, 1980
Givner LB, Baker CJ, Edwards MS: Type III group B streptococcus: Functional interaction with IgG subclass antibodies. J Infect Dis 155:532–539, 1987
Kim KS, Concepcion NF, Wass CA, Kang JH, Anthony BF: Demonstration of opsonic and protective activity of cord sera against type III group B streptococcus that are independent of type-specific antibody. Pediatr Res 24:628–632, 1988
Skvaril F, Roth-Wicky B, Barandun S: IgG subclasses in human gamma globulin preparations for intravenous use and their reactivity with staphylococcal protein A. Vox Sang 38:147–155, 1980
Skvaril F: The question of specificity in binding human IgG subclasses to protein A-Sepharose. Immunochemistry 10:871–872, 1976
Recht B, Frangione B, Franklin EC, Van Loghem E: Structural studies of a human 3 myeloma protein (GOE) that binds staph protein A. J Immunol 127:917–923, 1981
Shimizu A, Honzawa M, Ito S, Mizazaki T, Matumoto H, Nakamura H, Michaelson TE, Arata Y: NMR studies of the Fc region of human IgG1 and IgG3 immunoglobulins: Assignment of histidine resonances in the CH3 protein carrying G3M (st) allotypes. Mol Immunol 129:141, 1983
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kim, J.S., Kim, K.S., Wass, C.A. et al. A human IgG 3 is opsonicin vitro against type III group B streptococci. J Clin Immunol 10, 154–159 (1990). https://doi.org/10.1007/BF00917915
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00917915