Distinctive patterns of release of neuroendocrine peptides after nasal challenge of allergic subjects with ryegrass antigen
The concentrations of the neuropeptides substance P, somatostatin, and calcitonin gene-related peptide in human nasal secretions were quantified by radioimmunoassays, concurrently with that of histamine, in the course of nasal challenge of allergic and control subjects with ryegrass antigen to examine contributions of neuromediation of the tissue response. Each of theneuropeptides and histamine were detected in nasal lavage fluid prior to challenge. In allergic patients, but not normal controls, antigen evoked significant increases of 3-fold in histamine at 15–60 min, 1.5- to 4-fold in calcitonin gene-related peptide at 15 min-24 hr, and more than 2-fold in somatostatin at 6 hr, without altering the concentration of substance P in nasal lavage fluid. The identity of the neuropeptides was confirmed chromatographically. Thus calcitonin gene-related peptide may mediate nasal congestion directly and somatostatin may be one of the factors regulating the late involvement of basophils and mast cells in allergic rhinitis.
Key wordsNeuropeptides neuroimmunology somatostatin calcitonin gene-related peptide allergy
Unable to display preview. Download preview PDF.
- 1.Lundberg JM, Franco-Cereceda A, Hua X, Hokfelt T, Fischer JA: Co-existence of substance P and calcitonin generelated peptide-like immunoreactivities in sensory nerves in relation to cardiovascular and bronchoconstrictor effects of capsaicin. Eur J Pharmacol 108:315–319, 1985Google Scholar
- 2.Lundblad L: Protective reflexes and vascular effects in the nasal mucosa elicited by activation of capsaicin-sensitive substance P immunoreactive trigeminal neurons. Acta Physiol Scand Suppl 529:1–42, 1984Google Scholar
- 3.Hagermark O, Hokfelt T, Pernow B: Flare and itch induced by substance P in human skin. J Invest Dermatol 71:233–235, 1978Google Scholar
- 4.Goodman EC, Iversen LL: Calcitonin gene-related peptide: Novel neuropeptide. Life Sci 38:2169–2178, 1986Google Scholar
- 5.Payan DG, McGillis JP, Goetzl EJ: Neuroimmunology.In Advances in Immunology, FJ Dixon, KF Austen, L Hood, JW Uhr (eds). New York, Academic Press, 1986, pp 299–323Google Scholar
- 6.Druce HM, Wright RH, Rossoff D, Kaliner MA: Cholinergic nasal hyperreactivity in atopic subjects. Allergy Clin Immunol 76:445–452, 1985Google Scholar
- 7.Goetzl EJ, Chernov-Rogan T, Cooke MP, Renold F, Payan DG: Endogenous somatostatin-like peptides of rat basophilic leukemia cells. J Immunol 135:2707–2712, 1985Google Scholar
- 8.Goetzl EJ, Chernov-Rogan T, Furuichi K, Goetzl LM, Lee JY, Renold F: Neuromodulation of mast cell and basophil function.In Mast Cell Differentiation and Heterogeneity, AD Befus, J Bienenstock, JA Denberg (eds). New York, Raven Press, 1986, pp 223–229Google Scholar
- 9.Karlsson G, Pipkorn U, Andreasson L: Substance P and human nasal mucociliary activity. Clin Pharmacol 30:355–357, 1986Google Scholar