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Priming effects of leukotriene B4 on endothelial cell injury induced by tpa-activated leukocytes

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Abstract

Among arachidonic acid metabolites, leukotriene B4 (LTB4) plays an important role in inflammation, such as in the activation, adhesion, chemotaxis, and invasion of leukocytes. In this paper, we examined the effect of LTB4 on endothelial cell injury induced by polymorphonuclear leukocytes (PMNLs).51Crrelease, a marker of cellular injury, was elicited from prelabeled endothelial cells when the cells were cocultured with PMNLs activated by phorbol ester (TPA, 12-0-tetradecanoyl-phorbol-13-acetate). Under this condition, pretreatment of PMNLs with LTB4 enhanced their injury in a dose-dependent manner (0.2–2 μM). However, LTB4 alone at any dose could not induce any cellular injury. We also determined the amount of active oxygen species produced by PMNLs in response to TPA. The intensity of luminoldependent chemiluminescence, a marker of active oxygen production, in PMNLs was also increased by pretreatment with 1 μM LTB4. These data suggest that LTB4 enhances endothelial cell injury by the priming effect on active oxygen production in activated PMNLs.

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Authors and Affiliations

  1. Section of Physiological Chemistry, Graduate School, Tokyo Medical and Dental University, Yushima, Bunkyo-ku, 113, Tokyo, Japan

    Naoto Suda, Ikuo Morita & Sei -Itsu Murota

  2. Department of 2nd Orthodontics, School of Dentistry, Tokyo Medical and Dental University, Yushima, Bunkyo-ku, 113, Tokyo, Japan

    Naoto Suda & Takayuki Kuroda

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  1. Naoto Suda
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Suda, N., Morita, I., Kuroda, T. et al. Priming effects of leukotriene B4 on endothelial cell injury induced by tpa-activated leukocytes. Inflammation 16, 307–314 (1992). https://doi.org/10.1007/BF00917623

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