Advertisement

Inflammation

, Volume 14, Issue 2, pp 185–194 | Cite as

Comparison of abilities of recombinant interleukin-1α and -β and noninflammatory IL-1β fragment 163–171 to upregulate C3b receptors (CR1) on human neutrophils and to enhance their phagocytic capacity

  • James D. Ogle
  • J. Greg Noel
  • Ambikaipakan Balasurbramaniam
  • R. Michael Sramkoski
  • Cora K. Ogle
  • J. Wesley Alexander
Original Articles

Abstract

Both recombinant IL-1α and -β caused an upregulation of C3b receptors (CR1) on human neutrophils and caused a receptor-mediated enhancement of phagocytosis of C3b·IgG-coated microspheres by these leukocytes. Theα andβ forms of the recombinant cytokine were of comparable potency regarding CR1 upregulation, although both generally had less than 25% of the potency of FMLP in this respect. Recombinant IL-1β was slightly more potent than theα form of the cytokine regarding phagocytosis of opsonized microspheres and, again, both forms were less potent than FMLP in causing an enhancement of phagocytosis by neutrophils. The synthetic noninflammatory immunostimulatory nonapeptide corresponding to residues 163–171 of IL-1β was completely inert with respect to upregulation of CR1 on neutrophils and the enhancement of phagocytosis by these cells. Thus this domain in the intact IL-1β molecule apparently is not involved in CR1 upregulation and the ensuing enhancement in phagocytosis by neutrophils, although it is apparently important in the immunostimulatory activity regarding the proliferation of lymphocytes.

Keywords

Public Health Internal Medicine Human Neutrophil Phagocytic Capacity Immunostimulatory Activity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Ogle, J. D., G.Noel, C. K.Ogle, R. M.Sramkoski, and J. W.Alexander. 1989. The effects of cytokines on the expression of C3b receptors (CRI) on neutrophils and on the phagocytosis of opsonized polystyrene microspheres by these leukocytes (submitted).Google Scholar
  2. 2.
    Ozaki, Y., T. Ohashi, andS. Kume. 1987. Potentiation of neutrophil function by recombinant DNA-produced interleukin la.J. Leukocyte Biol. 42:621–627.Google Scholar
  3. 3.
    Georgilis, K., C. Schaefer, C. A. Dinarello, andM. S. Klempner. 1987. Human recombinant interleukin 1β has no effect on intracellular calcium or on functional responses of human neutrophils.J. Immunol. 138(10):3403–3407.Google Scholar
  4. 4.
    Maloff, B. L., J. E. Shaw, andD. Fox. 1988. A chemotaxis assay using human polymorphonuclear leukocytes stimulated by IL-1.J. Immunol. Methods 112:145–146.Google Scholar
  5. 5.
    Hsieh, K.-H., andK.-H. Lue. 1987. Defective monokine production and decreased responsiveness of polymorphonuclear leukocytes to recombinant interleukin-1 in asthmatic patients.J. Clin. Immunol. 7(3):203–209.Google Scholar
  6. 6.
    Minami, A., K. Fujimoto, Y. Ozaki, andS. Nakamura. 1988. Augmentation of host resistance to microbial infections by recombinant human interleukin-1α.Infect. Immun. 56(12):3116–3120.Google Scholar
  7. 7.
    Seow, W. K., Y. H. Thong, andA. Ferrante. 1987. Macrophage-neutrophil interactions: Contrasting effects of the monokines interleukin-1 and tumour necrosis factor (cachectin) on human neutrophil adherence.Immunology 62:357–361.Google Scholar
  8. 8.
    Antoni, G., R. Presenting F. Perin, A. Tagliabue, P. Ghiara, S. Censini, G. Volpini, L. Villa, andD. Boraschi. 1986. A short synthetic peptide fragment of human interleukin 1 with immunostimulatory but not inflammatory activity.J. Immunol. 137:3201–3204.Google Scholar
  9. 9.
    Nencioni, L., L. Villa, A. Tagliabue, G. Antoni, R. Presentini, F. Perin, S. Silvestri, andD. Borashci. 1987. In vivo immunostimulating activity of the 163–171 peptide of human IL-1β.J. Immunol. 139:800–804.Google Scholar
  10. 10.
    Boraschi, D., L. Nencioni, L. Villa, S. Censini, P. Bossu, P. Ghiara, R. Presentini, F. Perin, D. Frasca, G. Doria, G. Forni, T. Musso, M. Giovarelli, P. Ghezzi, R. Bertini, H. O. Besedovsky, A. Del Rey, D. Sipe, G. Antoni, S. Silvestri, andA. Tagliabue. 1988. In vivo stimulation and restoration of the immune response by the noninflammatory fragment 163–171 of human interleukin 1β.J. Exp. Med. 168:675–686.Google Scholar
  11. 11.
    Boraschi, D., G. Volpini, L. Villa, L. Nencioni, G. Scapigliati, D. Nucci, G. Antoni, G. Matteucci, F. Cioli, andA. Tagliabue. 1989. A monoclonal antibody to the IL-1β peptide 163–171 blocks adjuvanticity but not pyrogenecity of IL-1β in vivo.J. Immunol. 143:131–134.Google Scholar
  12. 12.
    Alexander, J. W., D. B. Windhorst, andR. A. Good. 1968. Improved tests for the evaluation of neutrophil function in human disease.J. Lab. Clin. Med. 72:136–148.Google Scholar
  13. 13.
    Ferrante, A., andY. H. Thong. 1978. A rapid one-step procedure for purification of mononuclear and polymorphonuclear leukocytes for human blood using a modification of the Hypaque-Ficoll technique.J. Immunol. Methods 24:389–393.Google Scholar
  14. 14.
    Ogle, J. D., J. G. Noel, R. M. Sramkoski, C. K. Ogle, andJ. W. Alexander. 1988. Phagocytosis of opsonized fluorescent microspheres by human neutrophils. A two-color flow cytometric method for the determination of attachment and ingestion.J. Immunol. Methods 115:17–29.Google Scholar
  15. 15.
    Ogle, J. D., C. K. Ogle, J. G. Noel, P. Hurtubise, andJ. W. Alexander. 1985. Studies on the binding of C3b-coated microspheres to human neutrophils.J. Immunol. Methods 76:47–62.Google Scholar
  16. 16.
    Balasubramaniam, A., P. C. Andrews, V. Renugopalakrishnan, andD. F. Rigel. 1988. Glycine-extended anglerfish peptide YG (aPY) a neuropeptide Y (NPY) homologue may be a precursor of a biologically active peptide.Peptides 10:581–585.Google Scholar
  17. 17.
    Knoig, W., andR. Geiger. 1970. A new method for synthesis of peptides: activation of the carboxyl group with dicyclohexylcarbodiimide using 1-hydroxybenzotriazoles as additives.Chem. Ber. 103:788–798.Google Scholar
  18. 18.
    Ogle, J. D., G.Noel, C. K.Ogle, R. M.Sramkoski, and J. W.Alexander. 1989. The effects of platelet activating factor and arachidonate metabolites on the expression of receptors on neutrophils and on the phagocytosis of opsonized polystyrene microspheres by these leukocytes (submitted).Google Scholar
  19. 19.
    Okada, D., andE. J. Brown. 1988. Sodium fluoride reveals multiple pathways for regulation of surface expression of the C3b/C4b receptor (CR1) on human polymorphonuclear leukocytes.J. Immunol. 140:878–884.Google Scholar
  20. 20.
    Jones, D. H., D. C. Anderson, B. L. Burr, H. E. Rudloff, W. Smith, S. S. Krater, andF. C. Schmalsteig. 1988. Quantitation of intracellularMac-1 (CD11b/DC18) pools in human neutrophils.J. Leukocyte Biol. 44:535–544.Google Scholar
  21. 21.
    Miller, L. J., D. F. Bainton, N. Borregaard, andT. A. Springer. 1987. Stimulated mobilization of monocyte Mac-1 and p150,95 adhesion proteins from an intracellular vesicular compartment to the cell surface.Clin. Invest. 80:535–544.Google Scholar
  22. 22.
    Rossi, F., D. Bianca, M. Grzeskowiak, andF. Bazzoni. 1989. Studies on molecular regulation of phagocytosis in neutrophils.J. Immunol. 142:1652–1660.Google Scholar

Copyright information

© Plenum Publishing Corporation 1990

Authors and Affiliations

  • James D. Ogle
    • 1
    • 3
  • J. Greg Noel
    • 3
  • Ambikaipakan Balasurbramaniam
    • 2
  • R. Michael Sramkoski
    • 3
  • Cora K. Ogle
    • 2
    • 3
  • J. Wesley Alexander
    • 2
    • 3
  1. 1.Departments of Molecular Genetics, Biochemistry and MicrobiologyUniversity of Cincinnati Medical CenterCincinnati
  2. 2.Department of SurgeryUniversity of Cincinnati Medical CenterCincinnati
  3. 3.Cincinnati UnitThe Shriners Burns InstituteUSA

Personalised recommendations