, Volume 3, Issue 1, pp 111–116 | Cite as

Restoration of methotrexate-suppressed oxazolone-induced contact sensitivity with levamisole

  • D. E. Griswold
  • D. T. Walz


The effect of levamisole upon cell-mediated immunity was investigated using the oxazolone-induced contact sensitivity response in immunosuppressed and nonimmunosuppressed C57B1 male mice. Mice were sensitized to oxazolone on day 0, and where appropriate, methotrexate (1 mg/kg, p.o.) was administered on days 1 and 2. On day 3, levamisole (5–50 mg/kg, p.o., base) was administered. One hour later, the animals were challenged with oxazolone on the left hindpaw. Twenty-four hours after challenge, the resulting edema was read plethysmographically. Levamisole, in the absence of immunosuppression, had no significant effect upon the oxazolone response, whereas, in the face of immunosuppression, restoration of oxazolone responsiveness was observed. These results were suggested to be due to (1) the ability of levamisole to stimulate opposing suppressor and effector components of the oxazolone response coupled with (2) an apparent alteration of suppressor influence by methotrexate allowing levamisole to enhance an unencumbered effector cell population.


Public Health Internal Medicine Cell Population Methotrexate Effector Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Scherurmans, Y. 1975. Levamisole in rheumatoid arthritis.Lancet 1:111.Google Scholar
  2. 2.
    Basch, C.M., andL.E. Spitler. 1975. The effects of levamisole in rheumatoid arthritis.Arthritis Rheum.18:385.Google Scholar
  3. 3.
    McGill, P.E. 1976. Levamisole in rheumatoid arthritis.Lancet 2:149.Google Scholar
  4. 4.
    Dinai, Y., andM. Pras. 1975. Levamisole in rheumatoid arthritis.Lancet 2:556.Google Scholar
  5. 5.
    Levy, J., andB. Miller. 1976. Deficient cell-mediated immune responses in rheumatoid arthritis and reversal with levamisole.Arthritis Rheum.19:807.Google Scholar
  6. 6.
    Huskisson, E.C., P.A. Dieppe, J. Scott, J. Trapnell, H.W. Balme, andD.A. Willoughby. 1976. Immunostimulant therapy with levamisole for rheumatoid arthritis.Lancet 2:393.Google Scholar
  7. 7.
    Kint, A., andL. Verlinder. 1974. Levamisole for recurrentHerpes liabialis.N. Engl. J. Med. 291:308.Google Scholar
  8. 8.
    Symoens, J., andJ. Brugmans. 1974. Treatment of recurrent aphthous stomatitis and herpes with levamisole.Br. Med. J. 4:592.Google Scholar
  9. 9.
    Rojas, A.F., E. Mickiewica, J.N. Feierstein, H. Glait, andA.J. Olivari. 1976. Levamisole in advanced human breast cancer.Lancet 1:211.Google Scholar
  10. 10.
    Levo, Y., V. Rotter, andB. Ramst. 1975. Restoration of cellular immune response by levamisole in patients with Hodgkin's disease.Biomedicine 23:198.Google Scholar
  11. 11.
    Arnold, H.L., Jr. 1976. Immunotherapy with levamisole.N. Engl. J. Med. 294:447.Google Scholar
  12. 12.
    Ward, H.W.C. 1976. Levamisole in the treatment of cancer.Lancet 1:594.Google Scholar
  13. 13.
    Amery, W.K. 1976. Levamisole (NSC-177023) in clinical immunotherapy.Cancer Chemother. Rep. 60:217.Google Scholar
  14. 14.
    Griswold, D.E., andD.T. Walz. 1977. Condition dependent immunoregulatory control of contact sensitivity by levamisole.Inflammation 2:227.Google Scholar
  15. 15.
    Renoux, G., M. Renoux, M.H. Teller, S. McMahon, andJ.M. Guillaumin. 1976. Potentiation of T-cell mediated immunity by levamisole.Clin. Exp. Immunol. 25:288.Google Scholar
  16. 16.
    Thulin, H., K. Thestrup-Pedersen, andJ. Ellegaard. 1975. In vitro parameters of cell-mediated immune reactions in healthy individuals following immune stimulation attempts with levamisole.Acta Allergol. 30:9.Google Scholar
  17. 17.
    Griswold, D.E., J.A. DiLorenzo, andP. Calabresi. 1974. Quantification and pharmacological dissection of oxazolone-induced contact sensitivity in the mouse.Cell. Immunol. 11:198.Google Scholar
  18. 18.
    Walz, D.T., M.J. DiMartino, J.K. Kuch, andW. Zuccarello. 1971. Adjuvantinduced arthritis in rats: I. Temporal relationship of physiological, biochemical and hematological parameters.Proc. Soc. Exp. Biol. Med. 136:907.Google Scholar
  19. 19.
    Lundy, J., andE.J. Lovett. 1976. Immuno-modulation of delayed hypersensitivity by thiabendazole.Proc. Am. Assoc. Cancer Res. 17:101.Google Scholar
  20. 20.
    Sampson, D., andA. Lui. 1976. The effect of levamisole on cell-mediated immunity and suppressor cell function.Cancer Res. 36:952.Google Scholar
  21. 21.
    Asherson, G.L., andM. Zembala. 1976. Suppressor T cells in cell-mediated immunity.Br. Med. Bull. 32:158.Google Scholar
  22. 22.
    Askenase, P.W., B.J. Hayden, andR.K. Gershon. 1975. Augmentation of delayedtype hypersensitivity by doses of cyclophosphamide which do not affect antibody responses.J. Exp. Med. 141:697.Google Scholar

Copyright information

© Plenum Publishing Corporation 1978

Authors and Affiliations

  • D. E. Griswold
    • 1
  • D. T. Walz
    • 1
  1. 1.Smith Kline & French LaboratoriesPhiladelphia

Personalised recommendations