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Pathogenesis of papain-induced emphysema in the hamster

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Abstract

In an effort to determine the mechanism of papain action in causing an emphysema-like lesion in hamsters, the number and types of cells and the activities of two lysosomal enzymes in the lung were determined after papain exposure. Three and four weeks after a 3-h exposure to an aerosol of 3% papain the following alterations in lung structure and function were observed: (1) the mean linear intercept, or average distance between adjacent alveoli, was increased; (2) the internal surface area declined; (3) the dynamic compliance was elevated at low breathing frequencies. The numbers of cells present free in the lung increased from a control value of 2.0±0.2×106 to 6.6±0.5×106 5 days after exposure. The freeβ-glucuronidase, a lysosomal enzyme, likewise increased over threefold during the first 3 days after exposure. These results are consistent with the hypothesis that papain induces an inflammatory-type response, and this may be in part responsible for inducing the lesion.

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Author notes

  1. Neil R. Ackerman

    Present address: Syntex Research, 3401 Hillview Avenue, 94304, Palo Alto, California

  2. Richard Corkey

    Present address: Harvard Medical School, Boston, Massachusetts

  3. David Perkins

    Present address: University of Connecticut School of Dentistry, Farmington, Connecticut

Authors and Affiliations

  1. Medical Research Laboratories, Pfizer Central Research, 06340, Groton, Connecticut

    Neil R. Ackerman, Richard Corkey & David Perkins

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  1. Neil R. Ackerman
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  2. Richard Corkey
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Ackerman, N.R., Corkey, R. & Perkins, D. Pathogenesis of papain-induced emphysema in the hamster. Inflammation 3, 49–58 (1978). https://doi.org/10.1007/BF00917321

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