Skip to main content
SpringerLink
Log in

Induction of plasma exudation and inflammatory cell infiltration by leukotriene C4 and leukotriene B4 in mouse peritonitis

  • Original Articles
  • Published:
Inflammation Aims and scope Submit manuscript

Abstract

Leukotriene induction of the fluid and cellular phases of the inflammatory response in the mouse was evaluated. Intraperitoneal injection of leukotriene C4 (LTC4 250 ng) led to dye extravasation but not polymorphonuclear leukocyte (PMN) infiltration, whereas injection of leukotriene B4 (LTB4 250 ng), led to PMN infiltration but not dye extravasation. The injection of both leukotrienes did not result in synergy. LTC4 did not appear to induce significant release or formation of chemotactic mediators, but the dye extravasation induced by LTC4 was inhibited by the vasoactive amine antagonist cyproheptadine and not by the eicosanoid inhibitors phenidone or naproxen. The response was markedly inhibited by the cytokine and eicosanoid inhibitors SK&F 86002 and SK&F 104493. PMN infiltration induced by LTB4 was not inhibited by SK&F 86002 or phenidone but was abrogated by colchicine treatment. LTB4 in this model did not appear to cause release or formation of vasoactive mediators. These leukotrienes appeared to be independent, complementary, and sufficient to mount a complete inflammatory response in the mouse.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Braquet, P., L. Toriqui, T. Y. Shen, andB. B. Vergraftig. 1987. Perspectives in platelet-activating factor research.Pharmacol. Rev. 39:97–145.

    Google Scholar 

  2. Williams, T. J., andJ. Morley. 1973. Prostaglandins as potentiators of increased permeability in inflammation.Nature 246:215–217.

    Google Scholar 

  3. Bray, M. A. 1986. Leukotrienes in inflammation.Agents Actions 19:87–99.

    Google Scholar 

  4. Plaut, M., andL. M. LicHtenstein. 1978. Cellular and chemical basis of the allergic inflammatory response: Component parts and control mechanisms.In Allergy, Principles and Practice. E. Middleton, Jr., C. E. Reed, and E. F. Ellis, editors. C. V. Mosby Co., St. Louis. 119–125.

    Google Scholar 

  5. Thureson-Klein, A., P. Hedqvist, A. Ohlen, J. Raud, andL. Lindbom. 1987. Leukotriene B4, platelet-activating factor and substance-P as mediators of acute inflammation.Pathol. Immunopathol. Res. 6:190–206.

    Google Scholar 

  6. Le, J., andJ. Vilcek. 1987. Tumor necrosis factor and interelukin 1: Cytokines with multiple overlapping biological activities.Lab. Invest.,56:234–248.

    Google Scholar 

  7. Konig, W., W. Schonfeld, M. Raulf, M. Knoller, J. Scheffer, andJ. Brom. 1990. The neutrophil and leukotrienes-role in health and disease.Eicosanoids 3:1–22.

    Google Scholar 

  8. Vinegar, R., J. F. Truax, andJ. C. Selph. 1976. Quantitative studies of the pathway to acute carrageenan inflammation.Fed. Proc. 35:2447–2456.

    Google Scholar 

  9. Walz, D. T., M. J. DiMartino, andA. Misher. 1971. Adjuvant-induced arthritis in rats. II. Drug effects on physiologic, biochemical and immunological parameters.J. Pharmacol. Exp. Ther. 178:223–231.

    Google Scholar 

  10. Regina, A. K., W. P. Charles, L. G. Gail, M. S. Robert, andF. S. William. 1985. Studies of leukotriene B4-specific binding and function in rat polymorphonuclear leukocytes: Absence of chemotactic response.J. Immunol. 134:3356–3363.

    Google Scholar 

  11. Griswold, D. E., E. F. Webb, M. A. Clark, andS. Mong. 1986. Phlogistic activity of leukotriene D4 in the mouse.Inflammation 10:1–7.

    Google Scholar 

  12. Young, J. M., D. A. Spires, C. J. Bedford, B. Wagner, S. J. Ballaron, andC. M. DeYoung. 1984. The mouse ear inflammatory response to topical arachidonic acid.J. Invest. Dermatol. 82:367–371.

    Google Scholar 

  13. Carlson, R. P., L. O'Neill-Davis, J. Chang, andA. J. Lewis. 1986. Modulation of mouse ear edema by cyclooxygenase and lipoxygenase inhibitors and other pharmacologic agents.Agents Actions 17:197–204.

    Google Scholar 

  14. Griswold, D. E., E. F. Webb, L. Schwartz, andN. Hanna. 1987. Arachidonic acid-induced inflammation: Inhibition by dual inhibitor of arachidonic acid metabolism, SK&F 86002.Inflammation 11:189–199.

    Google Scholar 

  15. Camp, R. D. R., A. A. Coutts, M. W. Greaves, A. B. Kay, andM. J. Walport. 1983. Responses of human skin to intradermal injection of leukotriene C4, D4, and B4.Br. J. Pharmacol. 80:497–502.

    Google Scholar 

  16. Thieroff, R., W. Frolich, andR. Ekerdt. 1988. Chemotactic activity of LTB4 in the skin of different laboratory animals in vivo.J. Invest. Dermatol. 90:254.

    Google Scholar 

  17. Sayers, T. J., T. A. Wiltrout, C. A. Bull, A. C. Denn III, A. M. Pilano, andB. Lokesh. 1988. Effects of cytokines in polymorphonuclear neutrophil infiltration in the mouse.J. Immunol. 141:1670–1677.

    Google Scholar 

  18. Griswold, D. E., S. Hoffstein, P. J. Marshall, E. F., Webb, L. Hillegass, P. E. Bender, andN. Hanna. 1989. Inhibition of inflammatory cell infiltration by bicyclic imidazoles, SK&F 86002 and SK&F 104493.Inflammation 13:727–739.

    Google Scholar 

  19. Lee, J. C., B. Votta, B. J. Dalton, D. E. Griswold, P. E. Bender, andN. Hanna. 1990. Inhibition of human monocyte IL-1 production by SK&F 86002.Intl. J. Immunother. 6:1–12.

    Google Scholar 

  20. Suarez, C. R., W. C. Pickett, D. H. Bell, D. K. McClintock, A. L. Oronsky, andS. S. Kerwar. 1987. Effect of low dose methotrexate on neutrophil chemotaxis induced by leukotriene B4 and complement C5a.J. Rheumatol. 14:9–11.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Respiratory/Inflammation Pharmacology, SmithKline Beecham Pharmaceuticals, P.O. Box 1539, 19406, King of Prussia, Pennsylvania

    D. E. Griswold, E. F. Webb & L. M. Hillegass

Authors
  1. D. E. Griswold
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. E. F. Webb
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. L. M. Hillegass
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Griswold, D.E., Webb, E.F. & Hillegass, L.M. Induction of plasma exudation and inflammatory cell infiltration by leukotriene C4 and leukotriene B4 in mouse peritonitis. Inflammation 15, 251–258 (1991). https://doi.org/10.1007/BF00917310

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00917310

Keywords

Search

Navigation