Abstract
We searched for antiidiotypes directed against anti-DNA in sera of healthy family members of lupus patients. Controls were healthy individuals without a personal or family history of lupus. No significant differences were noted between the family members' and the control group's sera with respect to binding to DNA or to non-anti-DNA F(ab′)2 fragments. Family members' sera had higher binding to anti-DNA F(ab′)2 and to normal IgG F(ab′)2 fragments (P<0.01). Sera of the family members had significantly higher binding to anti-DNA F(ab′)2 than to normal IgG F(ab′)2 fragments (P<0.0036). Inhibition experiments have shown that the antiidiotype is directed against the framework determinants and not against the antigen binding sites of the idiotype. The anti-idiotypic antibodies were directed against cross-reactive anti-DNA idiotypes and were not restricted to the idiotypes of the lupus proband. Age, sex, and blood relationship to the lupus patient did not influence the presence of antiidiotypes in the family members. The possible role of environmental factors in the induction of antiidiotypes and the role of the latter in regulating anti-DNA antibodies are discussed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Jerne NK: Towards a network theory of the immune system. Ann Immunol Inst Pasteur (Paris) 125C:373–379, 1974
Talal N: Autoimmunity and the immunologic network. Arth Rheum 21:853–861, 1978
Roitt IM, Male DK, Cooke A, Lydyard PM: Idiotypes and autoimmunity. Springer Semin Immunopathol 6:51–66, 1983
Abdou NI: The idiotype-anti-idiotype network in human autoimmunity. J Clin Immunol 5:365–369, 1985
Abdou NI, Wall H, Lindsley HB, Halsey JF, Suzuki T: Network theory in autoimmunity. In vitro suppression of serum anti-DNA antibody binding to DNA by anti-idiotypic antibody in systemic lupus erythematosus. J Clin Invest 67:1297–1304, 1981
Abdou NI, Wall H, Clancy J: The network theory in autoimmunity. In vitro modulation of DNA-binding cells by antiidiotype antibody present in inactive lupus sera. J Clin Immunol 1:234–240, 1981
Zouali M, Eyquem A: Idiotypic anti-idiotypic interactions in systemic lupus erythematosus. Demonstration of oscillatory levels of anti-DNA autoantibodies and reciprocal anti-idiotypic activity in a single patient. Ann Immunol Inst Pasteur 134C:377–391, 1983
Hatfield M, Evans M, Suenaga R, Hassanein KM, and Abdou NI: Anti-idiotypic antibody against anti-DNA in sera of laboratory personnel exposed to lupus sera or nucleic acids. Clin Exp Immunol 70:26–34, 1987
Zouali M, Eyquem A: Expression of anti-idiotypic clones against auto-anti-DNA antibodies in normal individuals. Cell Immunol 76:137–147, 1983
Taniguchi O, Chia DS, Barnett EV: Auto-anti-DNA antibodies from SLE patients and normals. J Rheumatol 11:291–297, 1984
Silvestris F, Bankhurst AD, Searles RP, Williams RC: Studies of anti-F(ab′)2 antibodies and possible immunologic control mechanisms in systemic lupus erythematosus. Arth Rheum 27:1387–1396, 1984
Silvestris F, Searles RP, Bankhurst AD, Williams RC: Family distribution of anti-F(ab′)2 antibodies in relatives with systemic lupus erythematosus. Clin Exp Immunol 60:329–338, 1985
DeHoratius RJ, Messner RP: Lymphocytotoxic antibodies in family members of patients with systemic lupus erythematosus. J Clin Invest 55:1254–1258, 1975
DeHoratius RJ, Pillarisetty R, Messner RP, Talal N: Antinucleic acid antibodies in systemic lupus erythematosus patients and their families. Incidence and correlation with lymphocytotoxic antibodies. J Clin Invest 56:1149–1154, 1975
Halpern R, Davidson A, Lazo A, Solomon G, Lahita R, Diamond B: Familial systemic lupus erythematosus. Presence of a cross-reactive idiotype in healthy family members. J Clin Invest 76:731–736, 1985
Isenberg DA, Shoenfeld Y, Walport M, Mackworth-Young C, Dudeney C, Todd-Pokropek A, Brill S, Weinberger A, Pinkas J: Detection of cross-reactive anti-DNA antibody idiotypes in the serum of systemic lupus erythematosus patients and of their relatives. Arth Rheum 28:999–1007, 1985
Tan EM, Cohen AS, Fries JF, Masi AT, McShane DJ, Rothfield NF, Schaller JG, Talal N, Winchester RJ: Revised criteria for the classification of systemic lupus erythematosus. Arth Rheum 25:1271–1277, 1982
Suenaga R, Evans M, Hatfield M, Abdou NI: Study of anti-DNA antibodies prepared by DNA cellulose or Cibacron blue chromatography. J Immunol Methods 93:131–139, 1986
Suenaga R, Munoz PA, Bright SW, Abdou NI: Spontaneous shift of anti-DNA antibodies in systemic lupus erythematosus. J Immunol 140:3508–3514, 1988
Silvestris F, Williams Jr RC, Searles RP: Human anti-F(ab′)2 antibodies and pepsin agglutinators react with Fv determinants. Scand J Immunol 23:499–508, 1986
Abdou NI, Suenaga R, Hatfield M, Evans M: Regulation of anti-DNA antibodies by anti-idiotypic antibodies in sera of families of lupus patients.In Idiotypes, M Reichlin, JD Capra (eds). New York, Academic Press, 1986, pp 95–107
Suenaga R, Hatfield M, Jones E, Jones JV, Abdou NI: Anti-idiotypes directed against anti-DNA in a family of a lupus patient. Correlation with HLA type (submitted for publication)
Lefvert AK, Pirskanen R, Svanborg E: Anti-idiotypic antibodies, acetylcholine receptor antibodies and disturbed neuromuscular function in healthy relatives to patients with myasthenia gravis. J Neuroimmunol 9:41–53, 1985
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Abdou, N.I., Suenaga, R., Hatfield, M. et al. Antiidiotypic antibodies against anti-DNA antibodies in sera of families of lupus patients. J Clin Immunol 9, 16–21 (1989). https://doi.org/10.1007/BF00917123
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00917123