Journal of Clinical Immunology

, Volume 2, Issue 2, pp 101–109 | Cite as

Effects of methyl-b12 on thein vitro immune functions of human T lymphocytes

  • Tsuyoshi Sakane
  • Shinsuke Takada
  • Hiroyuki Kotani
  • Tokugoro Tsunematsu
Original Articles


Studies were performed using anin vitro assay system to determine whether or not methyl-B12 could affect human T-cell function. When T cells were stimulated with phytohemagglutinin and allogeneic B cells, methyl-B12 did not enhance T-cell proliferation. In contrast, remarkable enhancing effects of methyl-B12 on the proliferative response to concanavalin A (Con A) and autologous B cells at suboptimal concentrations were observed, ranging from 0.1 to 10 µg/ml. Concentrations of methyl-B12 sufficient to enhance cellular proliferation were able to enhance the activity of helper T cells for immunoglobulin synthesis of B cells by pokeweed mitogen. Furthermore, the presence of methyl-B12 significantly potentiated the induction of suppressor cells in Con A-activated cultures. These results suggest that methyl-B12 could modulate lymphocyte function through augmenting regulatory T-cell activities.

Key words

Methyl-B12 immunomodulation T-cell proliferation helper T-cell function suppressor T-cell function 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Fukui S, Toraya T: Chemistry and biochemistry of vitamin B12 (corrinoid). Kagaku 28:786–797, 1973Google Scholar
  2. 2.
    Takimoto G, Yoshimatsu K, Isomura J, Ikeda T, Teramoto T, Osawa T: Immunomodulation by methyl B12. Igaku no Ayumi 112:588–590, 1980Google Scholar
  3. 3.
    Jerne NK: The immune system. Sci Am 229:52–60, 1973Google Scholar
  4. 4.
    Obata K: Synapse mechanism and mediators of nerve activity.In The Sensory and Nervous System, M Itoh (ed). Tokyo, Iwanami Shoten, 1974, pp 141–167Google Scholar
  5. 5.
    Sakane T, Steinberg AD, Reeves JP, Green I: Studies of immune functions of patients with systemic lupus erythematosus: Complement-dependent immunoglobulin M anti-thymus-derived cell antibodies preferentially inactive suppressor cells. J Clin Invest 63:954–965, 1979Google Scholar
  6. 6.
    Gronowicz E, Coutinho A, Melchers F: A plaque assay for all cells secreting Ig of a given type or class. Eur J Immunol 6:588–590, 1976Google Scholar
  7. 7.
    Sakane T, Steinberg AD, Green I: Studies of immune functions of patients with systemic lupus erythematosus. I. Dysfunction of suppressor T-cell activity related to impaired generation of, rather than response to, suppressor cells. Arth Rheum 21:657–664, 1978Google Scholar
  8. 8.
    Sagawa A, Abdou NI: Suppressor cell antibody in systemic lupus erythematosus: Possible mechanism for suppressor-cell dysfunction. J Clin Invest 63:536–539, 1979Google Scholar
  9. 9.
    Lohrmann H-P, Novikovs L, Graw RG Jr: Cellular interactions in the proliferative response of human T and B lymphocytes to phytomitogens and allogeneic lymphocytes. J Exp Med 139:1553–1567, 1974Google Scholar
  10. 10.
    Herscowitz HB, Sakane T, Steinberg AD, Green I: Heterogeneity of human suppressor cells induced by concanavalin A as determined in simultaneous assays of immune function. J Immunol 124:1403–1410, 1980Google Scholar
  11. 11.
    Sakane T, Honda M, Taniguchi Y, Kotani H: Separation of concanavalin A-induced human suppressor and helper T cells by the autologous erythrocyte rosette technique. J Clin Invest 68:447–453, 1981Google Scholar
  12. 12.
    Abdou NI, Pascual E, Racela LS: Suppressor T cell dysfunction and antisuppressor T cell antibody in active early rheumatoid arthritis (abstract). Arth Rheum 22:586, 1979Google Scholar
  13. 13.
    Keystone EC, Gladman DD, Buchaman R, Cane D, Poplonski L: Impaired antigen-specific suppressor cell activity in patients with rheumatoid arthritis. Arth Rheum 23:1246–1250, 1980Google Scholar
  14. 14.
    Sakane T, Taniguchi Y, Honda M, Kotani H: Suppressor T cell activity in rheumatoid arthritis and systemic lupus erythematosus. Ryumachi (Suppl)21:115–122, 1981Google Scholar
  15. 15.
    Norman T: Autoimmunity.In Basic and Clinical Immunology, 3rd ed, HH Fudenberg, DP Stites, JL Caldwell, JV Wells (eds). Los Altos, CA, Lange Medical, 1980, pp 220–231Google Scholar
  16. 16.
    Innes JB, Kuntz MM, Kim YT, Weksler ME: Induction of suppressor activity in the autologous mixed lymphocyte reaction and in cultures with concanavalin A. J Clin Invest 64:1608–1613, 1979Google Scholar
  17. 17.
    Sakane T, Green I: Specificity and suppressor function of human T cells responsive to autologous non-T cells. J Immunol 123:584–589, 1979Google Scholar
  18. 18.
    Cantor H, Boyse EA: Lymphocytes as models for the study of mammalian cellular differentiation. Immunol Rev 33:105–124, 1977Google Scholar
  19. 19.
    Minota M, Nishida M, Horiuchi A, Iwamura J: Effect of immunopotentiators on autologous rosette-forming cells in nude mice. Jap J Allergol 29:935–940, 1980Google Scholar
  20. 20.
    Bacon PA, Goldberg LS, Bluestone R: Experimental humoral and cellular immunity to hog intrinsic factor. Immunology 25:509–515, 1973Google Scholar
  21. 21.
    Kulapongs P, Vithayasai V, Suskind R, Olson RE: Cell-mediated immunity and phagocytosis and killing function in children with severe iron-deficiency anemia. Lancet 2:689–691, 1974Google Scholar
  22. 22.
    Hitzig WH, Kenny AB: The role of vitamin B12 and its transport globulins in the production of antibody. Clin Exp Immunol 20:105–111, 1975Google Scholar

Copyright information

© Plenum Publishing Corporation 1982

Authors and Affiliations

  • Tsuyoshi Sakane
    • 1
  • Shinsuke Takada
    • 1
  • Hiroyuki Kotani
    • 1
  • Tokugoro Tsunematsu
    • 1
  1. 1.Department of Internal MedicineShimane Medical UniversityIzumo, ShimaneJapan

Personalised recommendations