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Journal of Clinical Immunology

, Volume 2, Issue 2, pp 101–109 | Cite as

Effects of methyl-b12 on thein vitro immune functions of human T lymphocytes

  • Tsuyoshi Sakane
  • Shinsuke Takada
  • Hiroyuki Kotani
  • Tokugoro Tsunematsu
Original Articles

Abstract

Studies were performed using anin vitro assay system to determine whether or not methyl-B12 could affect human T-cell function. When T cells were stimulated with phytohemagglutinin and allogeneic B cells, methyl-B12 did not enhance T-cell proliferation. In contrast, remarkable enhancing effects of methyl-B12 on the proliferative response to concanavalin A (Con A) and autologous B cells at suboptimal concentrations were observed, ranging from 0.1 to 10 µg/ml. Concentrations of methyl-B12 sufficient to enhance cellular proliferation were able to enhance the activity of helper T cells for immunoglobulin synthesis of B cells by pokeweed mitogen. Furthermore, the presence of methyl-B12 significantly potentiated the induction of suppressor cells in Con A-activated cultures. These results suggest that methyl-B12 could modulate lymphocyte function through augmenting regulatory T-cell activities.

Key words

Methyl-B12 immunomodulation T-cell proliferation helper T-cell function suppressor T-cell function 

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Copyright information

© Plenum Publishing Corporation 1982

Authors and Affiliations

  • Tsuyoshi Sakane
    • 1
  • Shinsuke Takada
    • 1
  • Hiroyuki Kotani
    • 1
  • Tokugoro Tsunematsu
    • 1
  1. 1.Department of Internal MedicineShimane Medical UniversityIzumo, ShimaneJapan

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