Journal of Clinical Immunology

, Volume 9, Issue 3, pp 214–222 | Cite as

The influence of age, race, and gender on peripheral blood mononuclear-cell subsets in healthy nonsmokers

  • D. J. Tollerud
  • J. W. Clark
  • L. Morris Brown
  • C. Y. Neuland
  • L. K. Pankiw-Trost
  • W. A. Blattner
  • R. N. Hoover
Original Articles


To investigate the influence of age, race, and gender on the cellular immune system, we determined T-cell, B-cell, monocyte, natural killer (NK)-cell, and HLA-DR+-cell subsets in 266 nonsmokers from a population-based random sample of healthy adults using monoclonal antibodies and flow cytometry. Blacks had a lower total white blood-cell count than whites (P≤0.0001), due primarily to a decrease in granulocytes. There was no significant difference in absolute lymphocyte count between blacks and whites. Blacks had a higher proportion of CD19+ cells (Leu 12+ B cells) and a lower proportion of CD3+ cells (OKT3+ T cells) than whites (P≤0.01). Female sex and increasing age were independently associated with an increased percentage of CD4+ cells (OKT4A+ helper-inducer T-cell subset), resulting in a higher helper/suppressor ratio among women and older individuals (P≤0.05). Black race and increasing age were independently associated with an increased proportion of HLA-DR+ cells (P≤0.0001) which was not attributable to B cells or monocytes. No significant age, race, or gender effects were observed for CD14+ cells (Leu M3+ monocytes) or CD16+ cells (Leu 11A+ natural killer cells). These data demonstrate that age, race, and gender are each associated with significant differences in peripheral blood monouclear-cell subsets. Population-based data such as these provide an important foundation for future design and interpretation of human flow cytometry data.

Key words

Lymphocyte subsets normal values flow cytometry age race gender 


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Copyright information

© Plenum Publishing Corporation 1989

Authors and Affiliations

  • D. J. Tollerud
    • 1
  • J. W. Clark
    • 1
  • L. Morris Brown
    • 1
  • C. Y. Neuland
    • 2
  • L. K. Pankiw-Trost
    • 2
  • W. A. Blattner
    • 1
  • R. N. Hoover
    • 1
  1. 1.Environmental Epidemiology BranchNational Cancer InstituteBethesda
  2. 2.Braton BiotechRockville

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