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IgG anti-tetanus toxoid antibody synthesis by human bone marrow. I. Two distinct populations of marrow B cells and functional differences between marrow and peripheral blood B cells

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Abstract

This investigation uses a system for inducing and detecting anti-tetanus toxoid antibody (anti-TT) synthesis to study specific antibody (Ab) synthesis by bone marrow mononuclear cells (MC). We measured the amounts of anti-TT secreted and the number of B cells secreting antibody (Ab). The ELISA plaque detects single B cells secreting specific Ab. The results show that (1) spontaneous anti-TT secretion by MC is higher than spontaneous anti-TT secretion by peripheral blood lymphocytes (PBL) using an ELISA plaque (P<0.01); (2) spontaneous anti-TT production by MC correlated with the serum anti-TT titers as measured by an ELISA (r=0.75,P=0.005); (3) two types of marrow B cells were identified—one that spontaneously secretes anti-TT and another that produces anti-TT after TT-stimulation; (4) the frequency of anti-TT-secreting B cells is higher in MC than in PBL; (5) the amount of Ab secreted per marrow B cell is not different from that secreted by a peripheral B cell; and (6) marrow B cells could be induced to produce anti-TTin vitro up to 10 months without added cytokines. These results show that bone marrow is a major repository for differentiated B cells that spontaneously produce Abs to maintain circulating Abs titers and for memory B cells that can be induced to produce specific Ab.

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Authors and Affiliations

  1. Bone Marrow Transplant Program, Hematology and Oncology Departments of Medicine and Pediatrics, Wayne State University, Detroit, Michigan

    Lawrence G. Lum, Edith Burns, Marleen M. Janson, Paul J. Martin, Bernadette R. Giddings, Margaret C. Seigneuret, Jan E. Noges & Sandra C. Galoforo

  2. Division of Geriatrics, University of Wisconsin School of Medicine, Milwaukee Clinical Campus, Milwaukee, Wisconsin

    Lawrence G. Lum, Edith Burns, Marleen M. Janson, Paul J. Martin, Bernadette R. Giddings, Margaret C. Seigneuret, Jan E. Noges & Sandra C. Galoforo

  3. Fred Hutchinson Cancer Research Center and Division of Oncology, Department of Medicine, University of Washington, 98195, Seattle, Washington

    Lawrence G. Lum, Edith Burns, Marleen M. Janson, Paul J. Martin, Bernadette R. Giddings, Margaret C. Seigneuret, Jan E. Noges & Sandra C. Galoforo

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  1. Lawrence G. Lum
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  2. Edith Burns
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  3. Marleen M. Janson
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  7. Jan E. Noges
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  8. Sandra C. Galoforo
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Lum, L.G., Burns, E., Janson, M.M. et al. IgG anti-tetanus toxoid antibody synthesis by human bone marrow. I. Two distinct populations of marrow B cells and functional differences between marrow and peripheral blood B cells. J Clin Immunol 10, 255–264 (1990). https://doi.org/10.1007/BF00916701

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  • DOI: https://doi.org/10.1007/BF00916701

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