Summary
-
1.
A method was described for the separation of L-hydroxyproline from a gelatin hydrolyzate by the “rhodanilate” method with simultaneous isolation of L-proline.
-
2.
A method is proposed for the separation of L-proline from L-hydroxyproline and the preparation of each of these amino acids in the chromatographically pure state after their simultaneous separation from a hydrochloric acid hydrolyzate of gelatin.
Literature cited
M. Bergmann, J. Biol. Chem. 110, 471 (1935).
E. Fischer and E. Abderhalden, Ber. 37, 3072 (1904); E. Fischer and I. Reif, Liebigs Ann. Chem. 363, 122 (1908).
I. Kapfhammer and I. Mohu, Physiol. Chem. 306, 76 (1956).
M. Bergmann and S. W. Fox, J. Biol. Chem. 109, 317 (1935).
O. Kovacs, M. Halmes, and I. Bernath, Acta phys. et ehem. Szegcel 3, 118 (1957).
K. T. Poroshin, T. D. Kozarenko, and Yu. I. Khurgin, Bull. Acad. Sci. USSR, Div. Chem. Sci. (1956), 1500; H. Rydon and P. W. Smith, Nature 169, 922 (1952); F. Reindel and W. Hoppe, Ber. 87, 1103 (1954).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Shibnev, V.A., Kozarenko, T.D. & Poroshin, K.T. Isolation of L-proline and l-hydroxyproline by the “rhodanilate” method. Russ Chem Bull 8, 1096–1098 (1959). https://doi.org/10.1007/BF00916684
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00916684