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Journal of Clinical Immunology

, Volume 4, Issue 6, pp 439–444 | Cite as

Neutrophil-mediated cellular cytotoxicity triggered by immobilized aggregated IgG: Anin vitro model of cell injury during immune complex diseases

  • Franco Dallegri
  • Franco Patrone
  • Guido Frumento
  • Alberto Ballestrero
  • Carlo Sacchetti
Original Articles

Abstract

Normal human neutrophils were found to destroy ox red blood-cell targets when incubated on micropore filters coated with aggregated IgG, as determined by the51Cr release method. An intact neutrophil oxidative metabolism was essential for the cytotoxic event, since cells from patients with chronic granulomatous disease failed to exert any cytolysis. The target-cell destruction was prevented by catalase, azide, and cyanide and was enhanced by superoxide dismutase, suggesting involvement of the myeloperoxidase-hydrogen peroxide system. Neutrophil-mediated cytotoxicity was markedly amplified by the chemotactic peptideN-formyl-methionyl-leucylphenylalanine, as a result of an increased activity of the myeloperoxidase-hydrogen peroxide cytolytic system itself. This system of cytotoxicity provides a direct evidence for the neutrophil capacity of destroying bystander target cells under conditions simulating thein vivo immunologically mediated tissue injury and offers an excellent model to study events occurring during immune complex diseases.

Key words

Immune complex disease cytotoxicity neutrophils 

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Copyright information

© Plenum Publishing Corporation 1984

Authors and Affiliations

  • Franco Dallegri
    • 1
  • Franco Patrone
    • 1
  • Guido Frumento
    • 1
  • Alberto Ballestrero
    • 1
  • Carlo Sacchetti
    • 1
  1. 1.Second Medical ClinicUniversity of Genova Medical SchoolGenovaItaly

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