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Journal of Clinical Immunology

, Volume 4, Issue 6, pp 424–428 | Cite as

Autologous mixed lymphocyte reaction in man: XV. Cellular and molecular basis of deficient autologous mixed lymphocyte response in insulin-dependent diabetes mellitus

  • K. George Chandy
  • Arthur M. Charles
  • Ann Kershnar
  • Bruce Buckingham
  • Nancy Waldeck
  • Sudhir Gupta
Original Articles

Abstract

The autologous mixed lymphocyte response (AMLR) and the allogeneic mixed lymphocyte response were deficient in a subset of patients with newly diagnosed insulin-dependent diabetes mellitus (IDDM). Using a single set of HLA-identical twins, the cellular and molecular basis of deficient AMLR was investigated and appears to be due to a defect in both responder T cells and stimulator non-T cells. Interleukin-2 production was diminished in the patient but not in the healthy twin. Thein vitro addition of purified interleukin-2 enhanced the depressed AMLR in the diseased twin. This suggests that the deficient AMLR in IDDM may be in part due to a deficiency in the production of interleukin-2.

Key words

Insulin-dependent diabetes mellitus autologous mixed lymphocyte response interleukin-2 

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Copyright information

© Plenum Publishing Corporation 1984

Authors and Affiliations

  • K. George Chandy
    • 1
  • Arthur M. Charles
    • 2
  • Ann Kershnar
    • 3
  • Bruce Buckingham
    • 3
  • Nancy Waldeck
    • 2
  • Sudhir Gupta
    • 1
  1. 1.Division of Basic and Clinical Immunology, Department of MedicineUniversity of California, IrvineIrvine
  2. 2.Division of Endocrinology, Department of MedicineUniversity of California, IrvineIrvine
  3. 3.Children's Hospital of Orange CountyOrange

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