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Enhanced chemiluminescence production by phagocytosing neutrophils in psoriasis

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Abstract

Using serum-coated zymosan, the generation of reactive oxidants by measurement of chemiluminescence was shown to be significantly enhanced in isolated peripheral psoriatic neutrophils compared to normal controls. This response was observed irrespective of whether zymosan was opsonized with fresh autologous or normal AB serum. However, this increased activity was reduced with zymosan was opsonized with serum that was preheated at 56°C for 30 min. There was no statistical correlation of chemiluminescence activity with degranulation ofβ-glucuronidase in either normal or psoriatic subjects. In addition, chemiluminescence produced by normal cells was significantly increased when zymosan was opsonized with psoriatic serum. The plasma membrane-bound enzyme, NAD(P)H oxidase, which produces superoxide in response to phagocytic stimulation, was significantly increased in psoriatic neutrophils compared to normal controls. These data add further evidence for activated neutrophils in psoriasis.

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References

  1. Greaves, M. W. 1983. Neutrophil polymorphonuclears, mediators and the pathogenesis of psoriasis.Br. J. Dermatol. 109:115.

    Google Scholar 

  2. Sedgwick, J. B., P. R. Bergstresser, andE. R. Hurd. 1980. Increased granulocyte adherence in psoriasis and psoriatic arthritis.J. Invest. Dermatol. 74:81.

    Google Scholar 

  3. Wahba, A., H. A. Cohen, M. Bar-Eli, andR. Gallily. 1978. Enhanced chemotactic and phagocytic activities of leukocytes in psoriasis vulgaris.J. Invest. Dermatol. 71:186.

    Google Scholar 

  4. Bloomfield, F. J., andM. M. Young. 1983. Enhanced release of inflammatory mediators from lithium-stimulated neutrophils in psoriasis.Br. J. Dermatol. 109:9.

    Google Scholar 

  5. Glinski, W., Z. Zaresbka, S. Jablonska, J. Imiela, andJ. Nozarewski. 1980. The activity of polymorphonuclear leukocyte neutral proteinases and their inhibitors in patients with psoriasis treated with continuous peritoneal dialysis.J. Invest. Dermatol. 75:481.

    Google Scholar 

  6. Baboir, B. M. 1978. Oxygen-dependent microbial killing by phagocytes.N. Engl. J. Med. 298:659.

    Google Scholar 

  7. Yost, F. J., Jr., andI. Fricovich. 1974. Superoxide radicals and phagocytosis.Arch. Biochem. Biophys. 161:395.

    Google Scholar 

  8. Johnson, R. B., Jr., B. B. Keele, H. P. Misra, J. E. Lehmeyer, L. S. Webb, R. L. Baehner, andK. V. Rajagopalan. 1975. The role of superoxide anion generation in phagocytic bactericidal activity.J. Clin. Invest. 55:1357.

    Google Scholar 

  9. Sacks, T., C. F. Moldow, P. R. Craddock, T. K. Bowers, andH. S. Jacob. 1978. Oxygen radicals mediate endothelial cell damage by complement stimulated granulocytes.J. Clin. Invest. 61:1161.

    Google Scholar 

  10. Sedgwick, J. B., P. R. Bergstresser, andE. R. Hurd. 1981. Increased superoxide generation by normal granulocytes incubated in sera from patients with psoriasis.J. Invest. Dermatol. 76:158.

    Google Scholar 

  11. Schopf, R. E., P. Altmeyer, andE. M. Lemmel. 1982. Increased respiratory burst activity of monocytes and polymorphonuclear leukocytes in psoriasis.Br. J. Dermatol. 107:505.

    Google Scholar 

  12. Ferrante, A., andY. H. Thong. 1978. A rapid one-step procedure for purification of mononuclear and polymorphonuclear leukocytes from human blood using a modification of the Hypaque-Ficoll technique.J. Immunol. Methods 24:389.

    Google Scholar 

  13. Segal, A. W., andT. J. Peters. 1976. Characterization of the enzyme defect in chronic granulomatous disease.Lancet 1:1363.

    Google Scholar 

  14. Lowry, O. H., N. J. Rosebrough, A. L. Farr, andR. J. Randall. 1951. Protein measurements with Folin phenol reagent.J. Biol. Chem. 193:265.

    Google Scholar 

  15. Bloomfield, F. J., andM. M. Young. 1982. Influence of lithium and fluoride on degranulation from human neutrophils in vitro.Inflammation 6:257.

    Google Scholar 

  16. Goldstein, I. M., D. Ross, H. B. Kaplan, andG. Weissmann. 1975. Complement and immunoglobulins stimulate superoxide production by human leukocytes independently of phagocytosis.J. Clin. Invest. 56:1115.

    Google Scholar 

  17. Krueger, G. G., H. R. Hill, andW. W. Jederberg. 1978. Inflammatory and immune cell function in psoriasis—a subtle disorder.J. Invest. Dermatol. 71:189.

    Google Scholar 

  18. Cotterill, J. A., M. M. Roberts, R. Freeman, B. King, andK. Mostoufi. 1974. Aspects of polymorph function in psoriasis.Proc. R. Soc. Med. 67:874.

    Google Scholar 

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Authors and Affiliations

  1. Department of Clinical Medicine, St. James Hospital, Dublin, Ireland

    Frederick J. Bloomfield & Marjorie M. Young

  2. Department of Dermatology, Adelaide Hospital, Dublin, Ireland

    Frederick J. Bloomfield & Marjorie M. Young

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  1. Frederick J. Bloomfield
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  2. Marjorie M. Young
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Bloomfield, F.J., Young, M.M. Enhanced chemiluminescence production by phagocytosing neutrophils in psoriasis. Inflammation 12, 153–159 (1988). https://doi.org/10.1007/BF00916398

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