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Inflammation

, Volume 9, Issue 3, pp 285–295 | Cite as

Modulation of leukotaxis by ibuprofen

A quantitative determination in vivo
  • Takao Shimanuki
  • Robert M. Nakamura
  • Gere S. Dizerega
Original Articles

Abstract

The purpose of this study was to evaluate the rabbit anterior eye chamber as a quantitative measure of leukotaxis using51Cr-labeled homologous leukocytes and then to determine the effects of ibuprofen onN-formyl-methionyl-leucyl-phenylalanine (FMLP) -induced leukotaxis. Leukocyte accumulation was assessed at various intervals (0–24 h) after instillation of FMLP (10−4 M, 40μl) and at various doses (FMLP 10−6–10−4 M). New Zealand white rabbits (2.6–3.0 kg) were treated with ibuprofen for three days with the following regimens: 8.0, 17.5, 35.0, 70.0 mg/kg/day. Leukocyte quantitation was determined using a direct cell count and recovery of51Cr-labeled leukocytes from anterior eye chamber aspirations 3 h after their injection into the systemic circulation. FMLP induced a dose-dependent accumulation of leukocytes. Leukocyte influx into the anterior eye chamber increased between 2 and 4 h after FMLP instillation, peaking between 4 and 6 h, then resolving after 8 h. Ibuprofen inhibited leukocyte accumulation into the anterior eye chamber in a dose dependent fashion with a maximum (90.0±1.4%, X±SEM) inhibition with 70 mg/kg/day and an ID50 of 8 mg/kg/day. In conclusion, the anterior eye chamber FMLP-stimulated leukotaxis assay is useful to evaluate the role of pharmacologie agents. Here, ibuprofen was found to inhibit leukotaxis in a dose-dependent manner.

Keywords

Public Health Internal Medicine Cell Count Systemic Circulation Ibuprofen 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Publishing Corporation 1985

Authors and Affiliations

  • Takao Shimanuki
    • 1
  • Robert M. Nakamura
    • 1
  • Gere S. Dizerega
    • 2
  1. 1.The Second Department of Surgery Yamagata School of MedicineYamagata UniversityYamagataJapan
  2. 2.Livingston Reproductive Biology Laboratory Department of Obstetrics and GynecologyUniversity of Southern California School of MedicineLos Angeles

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