Skip to main content
SpringerLink
Log in

Glycoprotein biosynthesis during inflammation in normal and streptozotocin-induced diabetic rats

  • Original Articles
  • Published:
Inflammation Aims and scope Submit manuscript

Abstract

Extracellular glycoproteins play an important role in wound healing; yet little is known about glycoprotein biosynthesis and its regulation by insulin in inflammation. Using [1-14C] fucose as a marker, glycoprotein biosynthesis was studied in carrageenan-induced granuloma from diabetic and control rats. Fucose incorporation into glycoproteins was followed for 24 h after an intraperitoneal injection of the label. Radioactivity in trichloroacetic acid precipitable serum glycopoteins and saline-soluble and insoluble glycoproteins was assessed in five-, seven-, and ten-day-old granuloma tissues. Fucose incorporation was higher in soluble glycoproteins (P < 0.01) at all points in controls than in diabetic granulomas, and peak incorporation was reached in both groups on the seventh day. Incorporation of fucose into insoluble glycoproteins was higher in normals on the seventh day than in diabetics. Liver-, kidney-, and intestine-soluble glycoproteins showed a maximum incorporation on the seventh day, but no difference was noted between diabetic and normal rats. Incorporation of fucose in insoluble glycoproteins showed a gradual decline with the age of granuloma in all tissues from both groups, with the exception of the kidney. In the kidney, fucosylation of insoluble glycoproteins was decreased (P < 0.01) in diabetics compared to controls. These results indicate an active phase of biosynthesis, with an increase in glycosylation during inflammation that is probably insulin dependent.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Vane, J. R., andS. H. Ferreira, editors. 1978. Handbook of Experimental Pharmacology, Vol. 50, Part 1, Inflammation. Springer Verlag, New York, 1–784.

    Google Scholar 

  2. Nicoletis, C., S. Bazin, andL. M. Lous. 1977. Clinical and biochemical features of normal, defective, and pathologic scars.Clin. Plast. Surg. 4:347–359.

    Google Scholar 

  3. Peacock, E. E. 1984. Wound Repair, 3rd ed. W. B. Saunders, Philadelphia. 56–140.

    Google Scholar 

  4. Cohen, I. K., C. D. Moore, andR. F. Diegelmann 1979. Onset and localization of collegan synthesis during wound healing in open rat skin wounds.Proc. Soc. Exp. Biol. Med. 160:458–462.

    Google Scholar 

  5. Ivaska, K. 1981. Effect of extracellular glycosaminoglycans on the synthesis of collagen and proteoglycans by granulation tissue cells. A study with freshly isolated experimental granulation tissue cells of rat.Acta Physiol. Scand. Suppl. 494:1–53.

    Google Scholar 

  6. Borel, J. P. 1982. Clinical biochemistry of inflammation and the role of connective tissue.In XI International Congress of Clinical Chemistry. E. Kaiser, F. Gabe, M. M. Muller, M. Bayer, editors, W. Gruyter, Berlin. 673–685.

    Google Scholar 

  7. Viljanto, J., R. Penttinen, andJ. Raekallio. 1981. Fibronectin in early phases of wound healing in children.Acta Chir. Scand. 147:7–13.

    Google Scholar 

  8. Dolynchuk, K. N., andJ.M. Bowness. 1981. The early metabolism of noncollagenous glycoproteins during wound healing.J. Surg. Res. 31:218–224.

    Google Scholar 

  9. Koj, A., H. Kasperczyk, J. Bereta, andA. H. Gordon. 1982. Changes in the blood level and affinity to concanavalin A of rat plasma glycoproteins during acute inflammation and hepatoma growth.Biochem. J. 206:545–553.

    Google Scholar 

  10. Koj, A. 1974.In Structure and Function of Plasma Proteins, Vol. 1. A. C. Allison, editor. Plenum Press, New York. 73–131.

    Google Scholar 

  11. Gordon A. H. 1976.In Plasma Protein Turnover. R. Bianchir, G. Mariani, and A. S. McFarlane, editors. University Park Press, Baltimore. 381–394.

    Google Scholar 

  12. Miller, L. L. 1976. The definitive role of known hormones in the regulation of net biosynthesis of five acute-phase proteins (APPP) by the isolated perfused rat liver: Corticosera.In Protides of the Biological Fluids, Vol. 23. H. Peters, editor. Pergamon, Oxford. 461–471.

    Google Scholar 

  13. Bhandaru, R., S. R. Srinivasan, B. Radhakrishnamurthy, andG. S. Berenson. 1982. Effects of high fat-high cholesterol diet on plasma lipid levels and on erythrocyte membrane composition.Atherosclerosis 42:263–272.

    Google Scholar 

  14. Dalferes, E. R., Jr., B. Radhakrishnamurthy, andG. S. Berenson. 1980. Studies on glycoproteins from bovine aorta.Artery 7:85–98.

    Google Scholar 

  15. Lowry, O. H., N. J. Rosebrough, A. L. Farr, andR. J. Randall 1951. Protein measurement with the Folin phenol reagent.J. Biol. Chem. 193:265–275.

    Google Scholar 

  16. Bekesi, J. G., andR. J. Winzler. 1967. The metabolism of plasma proteins: Studies on the incorporation ofl-Fucose-1-14C into tissue and serum in the normal rat.J. Biol. Chem. 242:3873–3879.

    Google Scholar 

  17. Srinivasan, S. R., G. S. Berenson, andB. Radhakrishnamurthy. 1970. Glycoprotein changes in diabetic kidneys.Diabetes 19:171–175.

    Google Scholar 

  18. Berenson, G. S., B. Radhakrishnamurthy, E. R. Dalferes, Jr., H. Ruiz, S. R. Srinivasan, F. Plavidal, andF. Brickman. 1972. Connective tissue macromolecular changes in rats with experimentally induced diabetes and hyperinsulinism.Diabetes 21:733–743.

    Google Scholar 

  19. Coffey, J. W., O. N. Miller, andO. Z. Sellinoer. 1964. The metabolism of L-fucose in the rat.J. Biol. Chem. 293:4011–4017.

    Google Scholar 

  20. Manchester, K.L., andF. G. Young. 1961. Insulin and protein metabolism.Vitam. Horm. 19:95–132.

    Google Scholar 

  21. Alhadeff, J. A., andP. Watkins. 1983. Dolichyl phosphate-mannosyltransferase and dolichyl phosphate-n-acetylglucosaminyltransferase activities in liver preparations from normal controls and patients with cystic fibrosis and diabetes mellitus.Clin. Chim. Acta 134:1–9.

    Google Scholar 

  22. Urban, J., D. Chan, G. Schreiber 1979. A rat serum glycoprotein whose synthesis rate increases greatly during inflammation.J. Biol. Chem. 254:10565–10568.

    Google Scholar 

  23. Stout, R. W., E. L. Bierman, andR. Ross. 1975. The effect of insulin on the proliferation of cultered primate arterial smooth muscle cells.Circ. Res. 36:319–327.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Departments Medicine, Lousiana State University Medical Center, 1542 Tulane Avenue, 70112, New Orleans, Louisiana

    C. Sharma, E. R. Dalferes Jr., B. Radhakrishnamurthy, E. L. Rosen & G. S. Berenson

  2. Departments of Biochemistry, Lousiana State University Medical Center, 1542 Tulane Avenue, 70112, New Orleans, Louisiana

    B. Radhakrishnamurthy

Authors
  1. C. Sharma
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. E. R. Dalferes Jr.
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. B. Radhakrishnamurthy
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. E. L. Rosen
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. G. S. Berenson
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

This research was supported by funds from the National Heart, Lung and Blood Institute of the U.S. Public Health Service, HL-21649 and HL-02942, and the National Research and Demonstration Center-Arteriosclerosis (HL-15103).

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sharma, C., Dalferes, E.R., Radhakrishnamurthy, B. et al. Glycoprotein biosynthesis during inflammation in normal and streptozotocin-induced diabetic rats. Inflammation 9, 273–283 (1985). https://doi.org/10.1007/BF00916276

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00916276

Keywords

Search

Navigation