Skip to main content
SpringerLink
Log in

Peripheral blood and intrarenal phagocytic chemiluminescence during acute kidney graft rejection

  • Original Articles
  • Published:
Inflammation Aims and scope Submit manuscript

Abstract

During organ graft rejection, soluble mediators of inflammation are released into the polymorphs (PMNs) and monocytes recruited from the blood. One functional capacity of polymorphs and monocytes/macrophages is the production of cytotoxic activated oxygen species upon stimulation, which may contribute to the rejection process. Nothing is known about the influence of allograft rejection on this inflammatory cell property. Chemiluminescence (CL) allows measurement of respiratory burst capacity in small cell samples. Zymosan-induced and luminol-amplified CL of diluted whole blood, separated PMNs, and mononuclear cells from peripheral venous blood, as well as of intragraft phagocytes was measured after allogeneic and autologous kidney transplantation in untreated dogs, CL of separated PMNs, mononuclear cells, and intragraft phagocytes was significantly elevated during allograft rejection. In autologous kidneys transplanted to recipients of allografts, CL was also increased in the autologous grafts during rejection of the allogeneic ones, indicating a systemic alteration in phagocyte function.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Allen, R. C., andL. D. Loose. 1976. Phagocytic activation of a luminol-dependent chemiluminescence in rabbit alveolar and peritoneal macrophages.Biochem. Biophys. Res. Commun. 69:245–252.

    Google Scholar 

  2. Lomnitzer, R., A. Glover, andA. R. Rabson. 1977. The effect of PHA-activated MN-cell supernatants on polymorphonuclear leukocyte function.Clin. Exp. Immunol. 29:501–508.

    Google Scholar 

  3. Coleman, D. L., K. E. Culver, andJ. L. Ryan. 1984. Enhancement of macrophage immune and nonimmune receptor-mediated phagocytosis by a low-molecular-weight soluble factor from resident thymocytes.J. Immunol. 133:3121–3127.

    Google Scholar 

  4. Vadas, M. A., N. Nicola, A. F. Lopez, D. Metcalf, G. Johnson, andA. Pereira. 1984. Mononuciear cell-mediated enhancement of granulocyte function in man.J. Immunol. 133:202–207.

    Google Scholar 

  5. Weisbart, R. H., D. W. Golde, S. C. Clark, G. G. Wang, andJ. C. Gasson. 1985. Human granulocyle-macrophage colony-stimulating factor is a neutrophil activator.Nature 314:361–363.

    Google Scholar 

  6. Pace, J. L., S. W. Russell, R. D. Schreiber, A. Altmann, andD. H. Katz. 1982. Macrophage activation: Prirning activity from a T-cell hybridoma is attributable to interferon-γ.Proc. Natl. Acad. Sci. U.S.A. 80:3782–3786.

    Google Scholar 

  7. Nathan, C. F., H. W. Murray, M. E. Wiebe, andB. Y. Rubin. 1983. Identification of intetferon-γ as the lymphokine that activates human macrophage oxidation metabolism and antimicrobial activity.J. Exp. Med. 158:670–689.

    Google Scholar 

  8. Cross, A. S., andG. H. Lowell. 1978. Stimulation of polymorphonuclear bactericidal activity by supernatants of activated human mononuclear cells.Infect. Immunity 22:502–507.

    Google Scholar 

  9. Pichlmayr, R., andG. Tidow. 1981. Operationsmethoden und Operationstechnik-Standardverfahren. In Transplantationschirurgie. R. Pichlmayr, editor. Springer, Berlin, 529–568.

    Google Scholar 

  10. HĀyry, P., E. v. Willebrand, J. Ahonen, E. Eklund, andI. Lautenschlager. 1981. Monitoring of organ allograft rejection by transplant aspiration cytology.Ann. Clin. Res. 13:264–287.

    Google Scholar 

  11. BoYum, A. 1968. Separation of leukocytes from blood and bone marrow.Scand. J. Clin. Lab. Invest. 21(Suppl. 97):77–89.

    Google Scholar 

  12. Sachs, L. 1984. Angewandte Statistik, 6th ed. Springer, Berlin.

    Google Scholar 

  13. Janco, R. L., andD. English. 1983. Cyclosporine and human neutrophil function.Transplantation 35:501–503.

    Google Scholar 

  14. Solberg, C. O., T. Kalager, H. R. Hill, andJ. Gleite. 1982. Polymorphonuclear leukoyte function in bacterial and viral infections.Scand. J. Infect. Dis. 14:11–18.

    Google Scholar 

  15. Hancock, W. W., N. M. Thompson, andR. C. Atkins. 1983. Composition of interstitial cellular infiltrate identified by monoclonal antibodies in renal biopsies of rejecting human renal allografts.Transplantation 35:458–463.

    Google Scholar 

  16. Asher, N. L., R. Hoffmann, S. Chen, andR. L. Simmons. 1980. Specific and nonspecific infiltration of sponge matrix allografts by specifically sensitized cytotoxic lymphocytes.Cell Immunol. 52:38–47.

    Google Scholar 

  17. Tannenbaum, J. S., C. B. Anderson, G. A. Sicard, D. W. McKeel, andE. E. Etheredge. 1984, Prostaglandin synthesis associated with renal allografi rejection in the dog.Transplantation 37:438–443.

    Google Scholar 

  18. Metzger, Z., J. T. Hoffeld, andJ. J. Opperheim. 1981. Regulation by PGE2 of the production of oxygen intermediates by LPS-activated macrophages.J. Immunol. 127:1109–1113.

    Google Scholar 

  19. Sedgwick, J. B., M. L. Berube, andR. B. Zurier. 1985. Stimulus-dependent inhibition of Superoxide generation by prostaglandins.Clin. Immunol. Immunopathol. 34:205–215.

    Google Scholar 

  20. Griffin, J. D., S. C. Meuer, S. F. Schlossman, andE. L. Reinherz. 1984. T-cell regulation of myelopoiesis: Analysis at a clonal level.J. Immunol. 133:1863–1868.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute for Surgical Research, University of Munich Klinikum Grosshadern, Marchioninistrasse 15, D-8000, München 70, Federal Republic of Germany

    F. Schödel, F. Krombach, C. Hammer & W. Brendel

Authors
  1. F. Schödel
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. F. Krombach
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. C. Hammer
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. W. Brendel
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Schödel, F., Krombach, F., Hammer, C. et al. Peripheral blood and intrarenal phagocytic chemiluminescence during acute kidney graft rejection. Inflammation 10, 271–280 (1986). https://doi.org/10.1007/BF00916122

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00916122

Keywords

Search

Navigation