Advertisement

Inflammation

, Volume 8, Issue 2, pp 181–191 | Cite as

Effect of dimethyl sulfoxide on the in vitro and in vivo bactericidal activity of human and mouse neutrophils and mononuclear phagocytes

  • Charles J. Czuprynski
  • Peter M. Henson
  • Priscilla A. Campbell
Original Articles

Abstract

Dimethyl sulfoxide (DMSO) is reported to have antiinflammatory activity in various systems. Since resistance to bacterial infection can be thought of as a specialized type of inflamation, we were interested in determining the effect of DMSO on phagocyte bactericidal activity. The results indicated that in vitro DMSO treatment of human and mouse neutrophils and mononuclear phagocytes caused a dose-dependent inhibition of the killing ofEscherichia coli andListeria monocytogenes. However, pretreatment of mice with DMSO in vivo caused only a slight decrease in the subsequent in vitro bactericidal activity of neutrophils and macrophages from those mice. In addition, repeated injection of mice with a physiologically relevant dosage of DMSO did not enhance the lethality of eitherE. coli orL. monocytogenes, nor did it affect the clearance of a sublethalListeria challenge from the spleen and liver. These results suggest that clinical usage of DMSO should not predispose human subjects to bacterial infection.

Keywords

Public Health Internal Medicine DMSO Dimethyl Bactericidal Activity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Jiminez, R. A. H., andR. F. Willkens. 1982. Dimethyl sulfoxide: A perspective of its use in rheumatic diseases.J. Lab. Clin. Med 100:489–500.Google Scholar
  2. 2.
    Muther, R. S., andW. M. Bennett. 1980. Effects of dimethyl sulfoxide on renal function in man.J.A.M.A. 244:2081–2083.Google Scholar
  3. 3.
    Wood, D. C., andJ. Wood. 1975. Pharmocologic and biochemical considerations of dimethyl sulfoxide.Ann. N. Y. Acad. Sci. 243:7–19.Google Scholar
  4. 4.
    Yellowlees, P., C. Greenfield, andN. McIntyre. 1980. Dimethylsulphoxide-induced toxicity.Lancet 2:1004–1006.Google Scholar
  5. 5.
    Rubin, L. F. 1975. Toxicity of dimethyl sulfoxide alone and in combination.Ann. N. Y. Acad. Sci. 243:98–103.Google Scholar
  6. 6.
    Bartfeld, H., andA. Goldstein. 1975. Cell-mediated immunity: Its modulation by dimethyl sulfoxide.Ann. N. Y. Acad. Sci. 243:81–90.Google Scholar
  7. 7.
    Pestronk, A., andD. B. Drachman. 1980. Dimethyl sulphoxide reduces anti-receptor antibody titres in experimental myasthenia gravis.Nature 288:733–734.Google Scholar
  8. 8.
    Repine, J. E., J. W. Eaton, M. W. Anders, J. R. Hoidal, andR. B. Fox. 1979. Generation of hydroxyl radical by enzymes, chemicals and human phagocytes in vitro. Detection with the anti-inflammatory agent, dimethyl sulfoxide.J. Clin. Invest. 64:1642–1651.Google Scholar
  9. 9.
    Repine, J. E., R. B. Fox, andE. M. Berger. 1981. Dimethyl sulfoxide inhibits killing ofStaphylococcus aureus by polymorphonuclear leukocytes.Infect. Immun. 31:510–513.Google Scholar
  10. 10.
    Musson, R. A., H. Shafran, andP. M. Henson. 1980. Intracellar levels and stimulated release of lysosomal enzymes from human peripheral blood monocytes and monocytederived macrophages. RES:J. Reticuloendothel. Soc. 28:245–260.Google Scholar
  11. 11.
    Czuprynski, C. J., P. M. Henson, andP. A. Campbell. 1984. Killing ofListeria monocytogenes by inflammatory neutrophils and mononuclear phagocytes from immune and nonimmune mice.J. Leuk. Biol. 35:193–208.Google Scholar
  12. 12.
    Czuprynski, C. J., P. A. Campbell, andP. M. Henson. 1983. Killing ofListeria monocytogenes by human neutrophils and monocytes, but not by monocyte-derived macrophages.J. Reticuloendothel. Soc. 34:29–44.Google Scholar
  13. 13.
    Bruning, J. L., andB. L. Kintz. 1968. Computational Handbook of Statistics. Scott, Foresman, Glenview, Illinois. 201–204.Google Scholar
  14. 14.
    Absolom, D. R., C. J.van Oss, W. Zingg, andA. W. Neumann. 1982. Phagocytosis as a surface phenomenon: opsonization by aspecific absorption of IgG as a function of bacteria hydrophobicity.J. Reticuloendothel. Soc. 31:59–70.Google Scholar
  15. 15.
    Kearns. R. J., andD. I. Hinrichs. 1978. Heat-liable B cell mitogen obtained fromListeria monocytogenes. Infect. Immun. 22:676–680.Google Scholar
  16. 16.
    Klein, S. M., G. Cohen, andA. J. Cedarbraum. 1980. The interaction of hydroxyl radicals with dimethylsulfoxide produces formaldehyde.FEBS Lett. 116:220–222.Google Scholar
  17. 17.
    McBurney, M. W., E. M. V. Jones-Villenueve, M. K. S. Edwards, andP. J. Anderson. 1982. Control of muscle and neuronal differentiation in a cultured embryonal carcinoma cell line.Nature 299:165–167.Google Scholar
  18. 18.
    Fukui, Y. 1980. Formation of multinuclear cells induced by dimethyl sulfoxide: Inhibition of cytokinesis and occurrence of novel nuclear division inDictyostelium cells.J. Cell Biol. 86:181–189.Google Scholar
  19. 19.
    Alam, S. S., andD. L. Layman. 1982. Dimethyl sulfoxide as a cholesterol-lowering agent in cultured fibroblasts exposed to low density lipoproteins.Biochim. Biophys. Acta 710:306–313.Google Scholar
  20. 20.
    Zwingelstein, G., H. Tapiero, J. Portoukalian, andA. Fourcade. 1982. The effect of dimethylsulfoxide on the lipid composition of inducible and noninducible Friend leukemia cells.Biochem. Biophys. Res. Commun. 108:437–446.Google Scholar
  21. 21.
    Kunze, M., andH. J. Klein. 1971. The influence of dimethyl sulfoxide upon experimental staphylococcal infection of the swiss albino mouse.Zbl. Bakt. I. Abt. Orig. 216:175–184.Google Scholar
  22. 22.
    Runckel, D. N., andJ. R. Swanson. 1980. Effect of dimethyl sulfoxide on serum osmolality.Clin. Chem. 26:1745–1747.Google Scholar

Copyright information

© Plenum Publishing Corporation 1984

Authors and Affiliations

  • Charles J. Czuprynski
    • 1
    • 2
  • Peter M. Henson
    • 2
    • 4
  • Priscilla A. Campbell
    • 1
    • 3
    • 4
  1. 1.Department of MedicineNational Jewish Hospital and Research CenterDenver
  2. 2.Department of PediatricsNational Jewish Hospital and Research CenterDenver
  3. 3.Department of MedicineUniversity of Colorado Health Sciences CenterDenver
  4. 4.Department of PathologyUniversity of Colorado Health Sciences CenterDenver

Personalised recommendations