Inflammation

, Volume 11, Issue 2, pp 163–174 | Cite as

Luminol-independent chemiluminescence by phagocytes is markedly enhanced by dexamethasone, not by other glucocorticosteroids

  • Yukie Niwa
  • Kyoichi Somiya
  • Yoshiki Miyachi
  • Tadashi Kanoh
  • Tsuyoshi Sakane
Original Articles

Abstract

The effect of several glucocorticosteroids on the generation of reactive oxygen species (ROS) was examined. The ROS assessed were O 2 , H2O2, OH·, and chemiluminescence (CL) (determined in the presence or absence of luminol), generated by both opsonized zymosan-stimulated neutrophils or monocytes and by the xanthine-xanthine oxidase system. Except for luminol-independent CL, only high concentrations (10−4 M) of steroids could decrease each ROS. In contrast, luminol-independent CL generation in the phagocyte system was increased in a dose-dependent manner by the addition of dexamethasone, but not by any other steroid. Further, in lymphocyte cultures stimulated with Con A for four days, luminol-independent CL generation was demonstrated and enhanced by the addition of dexamethasone, although CL generation was not detected in the absence of dexamethasone. These findings provide evidence that CL does not always represent light specific to ROS, and they suggest the possibility that dexamethasone induces emission of light at sites of inflammation.

Keywords

Oxygen Public Health H2O2 Reactive Oxygen Species Steroid 

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Copyright information

© Plenum Publishing Corporation 1987

Authors and Affiliations

  • Yukie Niwa
    • 1
  • Kyoichi Somiya
    • 2
  • Yoshiki Miyachi
    • 3
  • Tadashi Kanoh
    • 4
  • Tsuyoshi Sakane
    • 1
  1. 1.Department of Internal MedicineShimane Medical UniversityJapan
  2. 2.Department of PediatricsHamamatsu Medical CenterJapan
  3. 3.Department of DermatologyJapan
  4. 4.Department of Internal MedicineKyoto UniversityJapan

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