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Inflammation

, Volume 10, Issue 2, pp 183–193 | Cite as

Influence of plasma proteins on activity of proinflammatory enzyme phospholipase A2

  • P. Vadas
  • E. Stefanski
  • W. Pruzanski
Original Articles

Abstract

The influence of plasma proteins on quantitation of the proinflammatory enzyme phospholipase A2 (PLA2) from rheumatoid synovial fluid was studied using two different assays. Human and bovine serum albumins increased the rate of PLA, hydrolysis of membrane-associated phospholipid substrates. In contrast, albumin profoundly inhibited the PLA2 hydrolysis of synthetic phospholipids in micellar dispersion. Other plasma proteins (α,β andγ-globulins) had minimal effect on PLA2 activity in either assay system. Since the presence of albumin may compromise estimation of PLA2 and of phospholipase-inhibitory proteins, the appropriate selection of assay conditions is obligatory for the accurate quantitation of their respective activities.

Keywords

Public Health Internal Medicine Albumin Bovine Serum Bovine Serum Albumin 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Publishing Corporation 1986

Authors and Affiliations

  • P. Vadas
    • 1
  • E. Stefanski
    • 1
  • W. Pruzanski
    • 1
  1. 1.Immunology Diagnostic and Research Centre Rheumatic Diseases Unit, Department of Medicine The Wellesley HospitalUniversity of TorontoTorontoCanada

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