Skip to main content
SpringerLink
Log in

Inflammation-associated events in liver nuclei during acute-phase reaction

  • Original Articles
  • Published:
Inflammation Aims and scope Submit manuscript

Abstract

Isolated liver nucleoli from rats undergoing turpentine-induced inflammation (acute-phase reaction) synthesize rRNA at a rate significantly higher than normal. This increase is associated with, and possibly preceded by, an enhanced methylation of RNA, which further increases when rRNA synthesis has reached a plateau level. Five hours after turpentine treatment, before clear activation of RNA synthesis and methylations, the nucleocytoplasmic transport of rRNA (largely 40S and 60S subunits) and the related ATPase activity of isolated nuclei are significantly increased. Apparently, posttranscriptional control is affected before transcription of rRNA during the onset of the acute-phase reaction: both kinds of events eventually contribute to the expansion of the ribosome population which occurs in the liver cells from rats undergoing an inflammatory process. All these processes are activated before the liver starts the synthesis of acute-phase proteins.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Koj, A. 1970. Synthesis and turnover of acute-phase reactants.In Energy Metabolism in Trauma. A Ciba Foundation Symposium. R. Porter and J. Knight, editors. Churchill, London. 79.

    Google Scholar 

  2. Kushner, I. 1982. The phenomenon of the acute-phase response.Ann. N. Y. Acad. Sci. 389:39.

    Google Scholar 

  3. Baglia, F. A., S. W. Kwan, andG. M. Fuller. 1981. Haptoglobin biosynthesis in rats. Immunological identification of polysomes synthesizing haptoglobin in the cytoplasm of liver cells.Biochim. Biophys. Acta 696:107.

    Google Scholar 

  4. Princen, J. M. G., W. Nieuwenhuizen, G. P. B. M. Mol-Backx, andS. H. Yap. 1981. Direct evidence of transcriptional control of fibrinogen and albumin synthesis in rat liver during the acute-phase response.Biochem. Biophys. Res. Commun,102:717.

    Google Scholar 

  5. Ricca, G. A., R. W. Hamilton, J. W. McLean, A. Conn, andJ. E. Kalinyak. 1981. Rat α1-Acid glycoprotein mRNA. Cloning of double-stranded cDNA and kinetics of induction of mRNA levels following acute inflammation.J. Biol. Chem. 256:10362.

    Google Scholar 

  6. Piccoletti, R., M. G. Aletti, F. Cajone, andA. Bernelli-Zazzera. 1984. The role of nuclei, polyribosomes and cytosol factors in the onset of the acute-phase reaction in the liver cell.Br. J. Exp. Pathol. 65:419.

    Google Scholar 

  7. Wolf, S., M. Sameshima, S. A. Liebhaber, andD. Schlessinger. 1980. Regulation of ribosomal ribonucleic acid levels in growing,3H-arrested and crisis-phase WI-38 human diploid fibroblasts.Biochemistry 19:3484.

    Google Scholar 

  8. Cooper, H. L., andE. M. Gibson. 1971. Control of synthesis and wastage of ribosomal ribonucleic acid in lymphocytes.J. Biol. Chem. 246:5059.

    Google Scholar 

  9. Luck, D. N., andT. H. Hamilton. 1975. Early estrogen action. Stimulation of the synthesis of methylated ribosomal and transfer RNAs.Biochim. Biophys. Acta 383:23.

    Google Scholar 

  10. Schiaffonati, L., L. Bardella, G. Cairo, V. Giancotti, andA. Bernelli-Zazzera. 1984. Mechanism of enhanced RNA synthesis in acute-phase rat liver and its relationship to chromatin structure.Biochem. J. 219:165.

    Google Scholar 

  11. Aletti, M. G., R. Piccoletti, andA. Bernelli-Zazzera. 1984. Release of rRNA from liver nuclei during the early stages of the acute-phase reaction.Biochim. Biophys. Acta 783:179.

    Google Scholar 

  12. Liau, M. C., M. E. Hunt, andR. B. Hurlbert. 1976. Role of ribosomal RNA methylases in the regulation of ribosome production in mammalian cells.Biochemistry 15:3158.

    Google Scholar 

  13. Schumm, D. E., andT. E. Webb. 1974. Modified messenger ribonucleic acid release from isolated hepatic nuclei after inhibition of polyadenylate formation.Biochem. J. 139:191.

    Google Scholar 

  14. Palayoor, R., D. E. Schumm, andT. E. Webb. 1981. Transport of functional messenger RNA from liver nuclei in a reconstituted cell-free system.Biochim. Biophys. Acta 654:201.

    Google Scholar 

  15. Yu, L. C., J. Racevskis, andT. E. Webb. 1972. Regulated transport of ribosomal subunits from regenerating rat liver nuclei in a cell-free system.Cancer Res. 32:2314.

    Google Scholar 

  16. Hazan, N., andR. McCauley. 1976. Effect of phenobarbitone on the nucleocytoplasmic transport of ribonucleic acid in vitro.Biochem. J. 156:665.

    Google Scholar 

  17. Clawson, G. A., M. Koplitz, B. Castler-Schechter, andE. Smuckler, 1978. Energy utilization and RNA transport: Their interdependence.Biochemistry 17:3747.

    Google Scholar 

  18. Grummt, I., andR. Lindigkeit. 1973. Preribosomal RNA synthesis in isolated rat liver nucleoli.Eur. J, Biochem. 36:244.

    Google Scholar 

  19. Widnell, C. C., andJ. R. Tata. 1964, A procedure for the isolation of enzymically active rat liver nuclei.Biochem. J. 92:313.

    Google Scholar 

  20. Burton, K. A. 1956. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of the deoxyribonucleic acid.Biochem. J. 62:315.

    Google Scholar 

  21. Almog, R., andT. L. Shirey. 1978. A modified orcinol test for the specific determination of RNA.Anal. Biochem. 91:130.

    Google Scholar 

  22. Layne, E. 1957. Spectrophotometric and turbidimetric methods for measuring protein.Methods Enzymol. 3:450.

    Google Scholar 

  23. Wen, L.-T., andK. Tsukada. 1983. Effect of ethionine on synthesis and methylation of ribosomal ribonucleic acid in regenerating rat liver.Biochim. Biophys. Acta 741:153.

    Google Scholar 

  24. Grummt, I. 1977. The effect of histidine starvation on the methylation of ribosomal RNA.Eur. J. Biochem. 79:133.

    Google Scholar 

  25. Schumm, D. E., andT. E. Webb. 1981. Insulin-modulated transport of RNA from isolated liver nuclei.Arch. Biochem. Biophys. 210:275.

    Google Scholar 

  26. Schumm, D. E., D. J. McNamara, andT. E. Webb. 1973. Cytoplasmic proteins regulating messenger RNA release from nuclei.Nature (London), New Biol. 245:201.

    Google Scholar 

  27. Schumm, D. E., M. A. Niemann, T. Palayoor, andT. E. Webb. 1979. In vivo equivalence of a cell-free system from rat liver for ribosomal RNA processing and transport.J. Biol. Chem. 254:12126.

    Google Scholar 

  28. Agutter, P. S. 1983. An assessment of some methodological criticisms of studies of RNA efflux from isolated nuclei.Biochem. J. 214:915.

    Google Scholar 

  29. Clawson, G. A., J. James, C. H. Woo, D. S. Foiend, D. Moody, andE. A. Smuckler. 1980. Pertinence of nuclear envelope nucleoside triphosphatase activity to ribonucleic acid transport.Biochemistry 19:2748.

    Google Scholar 

  30. Smuckler, A. E., M. Koplitz, andD. E. Smuckler. 1976. Isolation of animal cell nuclei.In Subnuclear Components. Preparation and Fractionation. G. B. Birnie, editor. Butterworth, London.1–57.

    Google Scholar 

  31. Anderson, M. W., N. R. Ballal, andH. Busch. 1977. Nucleoli of thioacetamide-treated liver as a model for studying regulation of preribosomal RNA synthesis.Biochem. Biophys. Res. Commun. 78:129.

    Google Scholar 

  32. Grummt, I., V. A. Smith, andF. Grummt. 1976. Amino acid starvation affects the initiation frequency of nucleolar RNA polymerase.Cell 7:439.

    Google Scholar 

  33. Lewin, R. 1984. Genes. New York, John Wiley & Sons.

    Google Scholar 

  34. Vaughan, M. H., R. Soeiro, J. R. Warner, andJ. E. Darnell. 1967. The effects of methionine deprivation on ribosome synthesis in HeLa cells.Proc. Natl. Acad. Sci. U.S.A. 58:1527.

    Google Scholar 

  35. Liau, M. C., D. W. Smith, andR. B. Hurlbert. 1975. Preferential inhibition by homopolyribonucleotides of the methylation of ribosomal ribonucleic acid and disruption of the production of ribosomes in rat tumor.Cancer Res. 35:2340.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Centro di Studio per la Patologia Cellulare del CNR, Istituto di Patologia Generale dell 'Università degli Studi di Milano, Via Mangiagalli No. 31, 20133, Milano, Italy

    Roberta Piccoletti, Maria Giovanna Aletti & Aldo Bernelli-Zazzera

Authors
  1. Roberta Piccoletti
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Maria Giovanna Aletti
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Aldo Bernelli-Zazzera
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Piccoletti, R., Aletti, M.G. & Bernelli-Zazzera, A. Inflammation-associated events in liver nuclei during acute-phase reaction. Inflammation 10, 109–117 (1986). https://doi.org/10.1007/BF00915993

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00915993

Keywords

Search

Navigation