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Distinct patterns of granulocyte luminol-dependent chemiluminescence response to lectins WGA and RCA-I

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Abstract

The chemiluminescence response was measured in human polymorphonuclear leukocytes (PMNLs) after stimulation with different concentrations of the lectinsTriticum vulgaris agglutinin (wheat germ agglutinin, WGA) andRicinus communis agglutinin I (RCA-I). The two lectins achieved distinct patterns of chemiluminescence; RCA-I evoked a dose-dependent response (0–15μg/ml) with respect to the initial rate of light emission, but the peak value was reached after different lengths of time. A maximum response was obtained after about 25 min with a concentration of 2–4μg/ml. By contrast, WGA caused a bimodal reaction after stimulus addition. In this case the first peak occurred after 15–20 min, and the second peak after around 60 min with maximum effect for 0.5 and 0.25–0.50μg/ml WGA, respectively. The PMNL response is discussed in relation to the regulation of the production of oxygen metabolites and in relation to the pathophysiologic consequences of lectin-mediated activation of phagocytic cells in intestinal epithelium.

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Authors and Affiliations

  1. Department of Medical Microbiology, Faculty of Health Sciences, University of Linköping, S-581 85, Linköping, Sweden

    Karl -Eric Magnusson, Claes Dahlgren & Anita Sjölander

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  1. Karl -Eric Magnusson
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  2. Claes Dahlgren
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  3. Anita Sjölander
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Magnusson, K.E., Dahlgren, C. & Sjölander, A. Distinct patterns of granulocyte luminol-dependent chemiluminescence response to lectins WGA and RCA-I. Inflammation 12, 17–24 (1988). https://doi.org/10.1007/BF00915888

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