, Volume 12, Issue 4, pp 285–295 | Cite as

Stimulation of release of prostaglandins and thromboxanes from isolated guinea pig lung cells by Bradykinin, f-Met-Leu-Phe, phorbol myristate, ionophore a23187, and leukotrienes

  • C. Robidoux
  • J. P. Pelé
  • J. Maclouf
  • P. Pradelles
  • P. Sirois
Original Articles


Guinea pig lungs were perfused for 15 min with a solution of protease type VII (0.05 mg/ml) and dispersed to yield a suspension of morphologically intact and metabolically active lung cells (over 250 × 106 cells per animal). The viability of these cells assessed with the Trypan blue exclusion technique was more than 80%. Treatment of these mixed cells (1 × 106 cells/ml) with chemicals such as phorbol myristate acetate (1 × 10−9 to 1 × 10−7 M), f-Met-Leu-Phe (5 × 10−6 to 1 x 10−4 M), and the calcium ionophore A23187 (3.1 × 10−7 to 2.5 × 10−6 M), and with autacoids such as bradykinin (1 × 10−5 to 1 × 10−4 M), leukotriene B4 (1 × 10−13 to 1 × 10−7 M), and leukotriene D4 (1 × 10−10 to 1 × 10−7 M) stimulated to a variable degree the release of prostaglandin E2 and thromboxane B2 (measured with a novel enzyme immunoassay). It is suggested that icosanoid release from the lungs is the result of direct chemical or hormonal stimulation of the cells and not a consequence of vascular changes. Studies are in progress to purify lung cell populations and characterize the cells responsible for the release of these icosanoids.


Prostaglandin Trypan Blue Bradykinin Thromboxane A23187 
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Copyright information

© Plenum Publishing Corporation 1988

Authors and Affiliations

  • C. Robidoux
    • 1
  • J. P. Pelé
    • 1
  • J. Maclouf
    • 1
  • P. Pradelles
    • 1
  • P. Sirois
    • 1
  1. 1.Département de Pharmacologie, Faculté de MédecineUniversité de SherbrookeSherbrookeCanada

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