Stimulation of release of prostaglandins and thromboxanes from isolated guinea pig lung cells by Bradykinin, f-Met-Leu-Phe, phorbol myristate, ionophore a23187, and leukotrienes

Abstract

Guinea pig lungs were perfused for 15 min with a solution of protease type VII (0.05 mg/ml) and dispersed to yield a suspension of morphologically intact and metabolically active lung cells (over 250 × 10⁶ cells per animal). The viability of these cells assessed with the Trypan blue exclusion technique was more than 80%. Treatment of these mixed cells (1 × 10⁶ cells/ml) with chemicals such as phorbol myristate acetate (1 × 10⁻⁹ to 1 × 10⁻⁷ M), f-Met-Leu-Phe (5 × 10⁻⁶ to 1 x 10⁻⁴ M), and the calcium ionophore A23187 (3.1 × 10⁻⁷ to 2.5 × 10⁻⁶ M), and with autacoids such as bradykinin (1 × 10⁻⁵ to 1 × 10⁻⁴ M), leukotriene B₄ (1 × 10⁻¹³ to 1 × 10⁻⁷ M), and leukotriene D₄ (1 × 10⁻¹⁰ to 1 × 10⁻⁷ M) stimulated to a variable degree the release of prostaglandin E₂ and thromboxane B₂ (measured with a novel enzyme immunoassay). It is suggested that icosanoid release from the lungs is the result of direct chemical or hormonal stimulation of the cells and not a consequence of vascular changes. Studies are in progress to purify lung cell populations and characterize the cells responsible for the release of these icosanoids.