Inflammation

, Volume 12, Issue 4, pp 285–295 | Cite as

Stimulation of release of prostaglandins and thromboxanes from isolated guinea pig lung cells by Bradykinin, f-Met-Leu-Phe, phorbol myristate, ionophore a23187, and leukotrienes

  • C. Robidoux
  • J. P. Pelé
  • J. Maclouf
  • P. Pradelles
  • P. Sirois
Original Articles

Abstract

Guinea pig lungs were perfused for 15 min with a solution of protease type VII (0.05 mg/ml) and dispersed to yield a suspension of morphologically intact and metabolically active lung cells (over 250 × 106 cells per animal). The viability of these cells assessed with the Trypan blue exclusion technique was more than 80%. Treatment of these mixed cells (1 × 106 cells/ml) with chemicals such as phorbol myristate acetate (1 × 10−9 to 1 × 10−7 M), f-Met-Leu-Phe (5 × 10−6 to 1 x 10−4 M), and the calcium ionophore A23187 (3.1 × 10−7 to 2.5 × 10−6 M), and with autacoids such as bradykinin (1 × 10−5 to 1 × 10−4 M), leukotriene B4 (1 × 10−13 to 1 × 10−7 M), and leukotriene D4 (1 × 10−10 to 1 × 10−7 M) stimulated to a variable degree the release of prostaglandin E2 and thromboxane B2 (measured with a novel enzyme immunoassay). It is suggested that icosanoid release from the lungs is the result of direct chemical or hormonal stimulation of the cells and not a consequence of vascular changes. Studies are in progress to purify lung cell populations and characterize the cells responsible for the release of these icosanoids.

Keywords

Prostaglandin Trypan Blue Bradykinin Thromboxane A23187 

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Copyright information

© Plenum Publishing Corporation 1988

Authors and Affiliations

  • C. Robidoux
    • 1
  • J. P. Pelé
    • 1
  • J. Maclouf
    • 1
  • P. Pradelles
    • 1
  • P. Sirois
    • 1
  1. 1.Département de Pharmacologie, Faculté de MédecineUniversité de SherbrookeSherbrookeCanada

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