International Ophthalmology

, Volume 17, Issue 6, pp 349–353 | Cite as

Calcein: A new dye for evaluation of the blood-retinal barrier by fluorophotometry

  • M. Reza Golshani
  • Bahram Khoobehi
  • Gholam A. Peyman
Section: New Developments, Products and Techniques


A vitreous fluorophotometry study was performed on rabbits to compare the characteristics of carboxyfluorescein and calcein with those of sodium fluorescein. Our results demonstrated that carboxyfluorescein and calcein attained mid vitreous concentrations similar in magnitude to fluorescein approximately one hour after injection of the dye. As long as three to four hours after injection of the dye, the mean fluorescence levels of calcein in the chorioretina were much higher than those of sodium fluorescein and carboxyfluorescein. These results point out that calcein has potential experimental and clinical use as a probe of the blood-ocular barrier as well as in experimental and clinical angiography of the fundus.

Key words

blood-retinal barrier calcein carboxyfluorescein fluorophotometry sodium fluorescein 


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Copyright information

© Kluwer Academic Publishers 1993

Authors and Affiliations

  • M. Reza Golshani
    • 1
  • Bahram Khoobehi
    • 1
  • Gholam A. Peyman
    • 1
  1. 1.LSU Eye CenterLouisiana State University Medical Center School of MedicineNew OrleansUSA

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