Skip to main content

Advertisement

SpringerLink
Log in

Sézary cells with an unusual phenotype, their modulation with 12-O-tetradecanoyl phorbol-13-acetate (TPA)in vitro, and their relationship to T-cell development

  • Original Articles
  • Published:
Journal of Clinical Immunology Aims and scope Submit manuscript

Abstract

Leukemic cells from a patient with Sézary syndrome were studied with a panel of monoclonal antibodies. Despite their typical morphology, the cells expressed an unusual surface phenotype. They simultaneously expressed OKT4 and OKT8 reactivity, indicating a more immature origin of the neoplastic cells than what has previously been observed in Sézary syndrome, but they were OKT6 negative. In response to 12-O-tetradecanoyl phorbol-13-acetate (TPA) the cells underwent phenotypic alterations, as the OKT4 reactivity was significantly reduced and the OKT8 expression was retained. Thus, following TPA, the cells expressed a surface phenotype consistent with the cytotoxic/suppressor T-cell subset in normal peripheral blood. The findings are discussed in relation to normal T-cell maturation and differentiation processes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Fialkow PJ: Clonal origin of human tumors. Biochim Biophys Acta 458:283–321, 1976

    Google Scholar 

  2. Godal TG, Funderud S: Human B-cell neoplasms in relation to normal B-cell differentiation and maturation processes. Adv Cancer Res 36:211–255, 1982

    Google Scholar 

  3. Reinherz EL, Kung PC, Goldstein G, Levey RH, Schlossman SF: Discrete stages of human intrathymic differentiation: Analysis of normal thymocytes and leukemic lymphoblasts of T-cell lineage. Proc Natl Acad Sci USA 77:1588–1592, 1980

    Google Scholar 

  4. Kung PC, Goldstein G: Functional and developmental compartments of human T lymphocytes. Vox Sang 39:121–127, 1980

    Google Scholar 

  5. Reinherz EL, Schlossman SF: Derivation of human T-cell leukemias. Cancer Res 41:4767–4770, 1981

    Google Scholar 

  6. Greaves MF, Rao J, Hariri G, Verbi W, Catovsky D, Kung P, Goldstein G: Phenotypic heterogeneity and cellular origins of T cell malignancies. Leukemia Res 5:281–299, 1981

    Google Scholar 

  7. Reinherz EL, Nadler LM, Sallan SE, Schlossman SF: Subset derivation of T-cell acute lymphoblastic leukemia in man. J Clin Invest 64:392–397, 1979

    Google Scholar 

  8. Greaves M, Delia D, Sutherland R, Rao J, Verbi W, Kemshead J, Hariri G, Goldstein G, Kung P: Expression of the OKT monoclonal antibody defined antigenic determinents in malignancy. Int J Immunopharmacol 3:283–300, 1981

    Google Scholar 

  9. Kung PC, Berger CL, Goldstein G, LoGerfo P, Edelson RL: Cutaneous T cell lymphoma: Characterization by monoclonal antibodies. Blood 57:261–266, 1981

    Google Scholar 

  10. Haynes BF, Metzgar RS, Minna JD, Bunn PA: Phenotypic characterization of cutaneous T-cell lymphoma. Use of monoclonal antibodies to compare with other malignant T cells. N Engl J Med 304:1319–1323, 1981

    Google Scholar 

  11. Reinherz EL, Nadler LM, Rosenthal DS, Moloney WC, Schlossman SF: T-cell-subset characterization of human TCLL. Blood 53:1066–1075, 1979

    Google Scholar 

  12. Delia D, Greaves MF, Newman RA, Sutherland DR, Minowada J, Kung P, Goldstein G: Modulation of T leukaemic cell phenotype with phorbol ester. Int J Cancer 29:23–31, 1982

    Google Scholar 

  13. Påhlman S, Odelstad L, Larsson E, Grotte G, Nilsson K: Phenotypic changes of human neuroblastoma cells in culture induced by 12-0-tetradecanoylphorbol-13-acetate. Int J Cancer 28:583–589, 1981

    Google Scholar 

  14. Tötterman TH, Nilsson K, Sundström C: Phorbol ester-induced differentiation of chronic lymphocytic leukaemia cells. Nature 288:176–178, 1980

    Google Scholar 

  15. Nagasawa K, Mak TW: Phorbol esters induce differentiation in human malignant T lymphoblasts. Proc Natl Acad Sci USA 77:2964–2968, 1980

    Google Scholar 

  16. Godal T, Lindmo T, Ruud E, Heikkilä R, Henriksen Aa, Steen H, Marton PF: Immunologic subsets in human B-cell lymphomas in relation to normal B-cell development. Haematol Blood Transfus 26:314–319, 1981

    Google Scholar 

  17. Böyum A: Separation of leucocytes from blood and bone marrow. With special reference to factors which influence and modify sedimentation properties of hemapoietic cells. Scand J Clin Lab Invest Suppl 97, 1968

  18. Kvaløy S, Godal T, Marton PF, Steen H, Brennhovd IO, Abrahamsen AF: Spontaneous (3H)-thymidine uptake in histological subgroups of human B-cell lymphomas. Scand J Haematol 26:221–234, 1981

    Google Scholar 

  19. Godal T, Lindmo T, Marton PF, Landaas TØ, Langholm R, Høie J, Foss Abrahamsen A: Immunological subsets in human B-cell lymphomas. Scand J Immunol 14:481–492, 1981

    Google Scholar 

  20. Heier HE: The influence of mechanical force on the rosette test for human T lymphocytes. Scand J Immunol 3:677–681, 1974

    Google Scholar 

  21. Beverley PCL, Callard RE: Distinctive functional characteristics of human “T” lymphocytes defined by E rosetting or a monoclonal anti-T cell antibody. Eur J Immunol 11:329–334, 1981

    Google Scholar 

  22. Beverley PCL, Callard RE: Re-definition of human T cells by monoclonal antibodies.In Protides of the Biological Fluids, Vol 29, H Peeters (ed). Oxford, Pergamon Press, 1981, pp 653–658

    Google Scholar 

  23. Reinherz EL, Kung PC, Goldstein G, Schlossman SF: Separation of functional subsets of human T cells by a monoclonal antibody. Proc Natl Acad Sci USA 76:4061–4065, 1979

    Google Scholar 

  24. Reinherz EL, Kung PC, Goldstein G, Schlossman SF: Further characterization of the human inducer T cell subset defined by monoclonal antibody. J Immunol 123:2894–2896, 1979

    Google Scholar 

  25. Kung PC, Goldstein G, Reinherz EL, Schlossman SF: Monoclonal antibodies defining distinctive human T cell surface antigens. Science 206:347–349, 1979

    Google Scholar 

  26. Reinherz EL, Schlossman SF: The differentiation and function of human T lymphocytes. Cell 19:821–827, 1980

    Google Scholar 

  27. Trowbridge IS, Omary MB: Human cell surface glycoprotein related to cell proliferation is the receptor for transferrin. Proc Natl Acad Sci USA 78:3039–3043, 1981

    Google Scholar 

  28. Ip SH, Rittershaus CW, Struzziero CC, Hoxie JA, Hoffman RA, Healey KW, Lifter J: Evaluation of E-rosetting human lymphocytes with OKT11 and other monoclonal antibodies. Blood 60:795–799, 1982

    Google Scholar 

  29. Funderud S, Lindmo T, Ruud E, Marton PF, Langholm R, Elgjo RF, Vaage S, Lie S, Godal T: Delineation of subsets in human B cell lymphomas by a set of monoclonal antibodies raised against B lymphoma cells. Scand J Immunol (in press), 1983

  30. Stashenko P, Nadler LM, Hardy R, Schlossman SF: Characterization of a human B lymphocyte specific antigen. J Immunol 125:1678–1685, 1980

    Google Scholar 

  31. Nadler LM, Ritz J, Hardy R, Pesando JM, Schlossman SF: A unique cell surface antigen identifying lymphoid malignancies of B cell origin. J Clin Invest 67:134–140, 1981

    Google Scholar 

  32. Gaudernack G, Curry R, Messner R: Rapid identification of human B-lymphocytes and monocytes with rhodamine-labeledBrucella Melitensis. J Immunol Methods 43:251–259, 1981

    Google Scholar 

  33. Kvaløy S, Marton PF, Høst H, Solheim BG, Godal T: Distribution of T-cell subsets identified by monoclonal antibodies in cell suspensions from lymph node biopsies of human B-cell lymphomas. Scand J Haematol 28:293–305, 1982

    Google Scholar 

  34. Lindmo T, Steen HB: Flow cytometric measurement of the polarization of fluorescence from intracellular fluorescein in mammalian cells. Biophys J 18:173–187, 1977

    Google Scholar 

  35. Ortaldo JR, Bonnard GD, Herberman RB: Cytotoxic reactivity of human lymphocytes cultured in vitro. J Immunol 119:1351, 1977

    Google Scholar 

  36. Broder S: Normal and malignant T cells in the regulation of inflammation and immunity. AAS 7:134–142, 1980

    Google Scholar 

  37. Broder S, Edelson RL, Lutzner MA, Nelson DL, Mac-Dermott RP, Durm ME, Goldman CK, Meade BD, Waldmann TA: The Sézary syndrome. A malignant proliferation of helper T cells. J Clin Invest 58:1297–1306, 1976

    Google Scholar 

  38. Lawrence EC, Broder S, Jaffe ES, Braylan RC, Dobbins WO, Young RC, Waldmann TA: Evolution of a lymphoma with helper T cell characteristics in Sézary syndrome. Blood 52:481–492, 1978

    Google Scholar 

  39. Foon KA, Naiem F, Saxon A, Stevens R, Gale RP: Leukemia of T-helper lymphocytes: Clinical and functional features. Leukemia Res 5:1–10, 1981

    Google Scholar 

  40. Catovsky D, Wechsler A, Matutes E, Gomez R, Bourikas G, Cherchi M, Pepys EO, Pepys MB, Kitani T, Hoffbrand AV, Greaves MF: The membrane phenotype of T-prolymphocytic Leukaemia. Scand J Haematol 29:398–404, 1982

    Google Scholar 

  41. Kjeldsberg CR, Marty J: Prolymphocytic transformation of chronic lymphocytic leukemia. Cancer 48:2447–2457, 1981

    Google Scholar 

  42. Janossy G, Tidman N, Papageorgiou ES, Kung PC, Goldstein G: Distribution of T lymphocyte subsets in the human bone marrow and thymus: An analysis with monoclonal antibodies. J Immunol 126:1608–1613, 1981

    Google Scholar 

  43. Reinherz EL, Cooper MD, Schlossman SF: Abnormalities of T cell maturation and regulation in human beings with immunodeficiency disorders. J Clin Invest 68:699–705, 1981

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratory for Immunology, Norsk Hydro's Institute for Cancer Research, The Norwegian Radium Hospital, Montebello, Oslo 3, Norway

    Stein Kvaløy & Tore Godal

  2. Department of Biophysics, Norsk Hydro's Institute for Cancer Research, Oslo, Norway

    Tore Lindmo & Catharina Davies

  3. Department of Internal Medicine, Ullevål Hospital, Oslo, Norway

    Finn Wisløff

Authors
  1. Stein Kvaløy
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Tore Lindmo
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Catharina Davies
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Finn Wisløff
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Tore Godal
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kvaløy, S., Lindmo, T., Davies, C. et al. Sézary cells with an unusual phenotype, their modulation with 12-O-tetradecanoyl phorbol-13-acetate (TPA)in vitro, and their relationship to T-cell development. J Clin Immunol 3, 212–221 (1983). https://doi.org/10.1007/BF00915345

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00915345

Key words

Search

Navigation