Journal of Clinical Immunology

, Volume 4, Issue 4, pp 304–311 | Cite as

Cell-mediated immunity to cytomegalovirus (CMV) and herpes simplex virus (HSV) antigens in the acquired immune deficiency syndrome: Interleukin-1 and interleukin-2 modifyin vitro responses

  • J. F. Sheridan
  • L. Aurelian
  • A. D. Donnenberg
  • T. C. Quinn
Original Articles

Abstract

Peripheral blood lymphocytes (PBL) were obtained from five patients with the acquired immune deficiency syndrome (AIDS), six homosexual males with lymphadenopathy, and five normal heterosexual controls. Modulation of virus-specific immunity was assayedin vitro by measuring the lymphocyte blastogenic response and the production of lymphokine (leukocyte inhibition factor; LIF) by PBL stimulated with herpes simplex virus (HSV) or cytomegalovirus (CMV) antigens in the presence or absence of interleukin-1 (IL-1) and interleukin-2 (IL-2). PBL from the control and lymphadenopathy subjects responded to both antigens in the lymphocyte transformation assay (LT) measured on day 7, and the responses were significantly enhanced in cultures grown in the presence of antigen and IL-2 (1 U/ml). PBL from the AIDS patients were unresponsive, but responsiveness was restored by the addition of IL-2. The addition of IL-1 (0.02 µg/ml) to antigen-stimulated PBL cultures failed to enhance the proliferative responses in all three study groups. LIF production was assayed in the supernatants from day 1 PBL cultures. LIF was not produced by PBL from AIDS patients grown in the presence of viral antigens, whereas three of five patients from the lymphadenopathy group, and three of five control subjects gave rise to positive responses. The addition of IL-1 to the antigenstimulated cultures enhanced LIF production in the control and lymphadenopathy groups but not in the AIDS patients. The addition of IL-2 did not modulate LIF production by antigen-stimulated PBL from the control oR AIDS patients while suppressing the LIF response of the similarly stimulated PBL from the lymphadenopathy patients.

Key words

acquired immune deficiency syndrome (AIDS) cellular immunity (CMI) interleukin-1 interleukin-2 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Update on Acquired Immune Deficiency Syndrome (AIDS)—United States: Morbid Mortal Week Rep 31:507–514, 1982Google Scholar
  2. 2.
    Gottlieb MS, Schroff R, Schanker HM, Weisman JD, Fan PT, Wolf RA, Saxon A: Pneumocystis carinii pneumonia and mucosal condidiasis in previously healthy homosexual men. N Engl J Med 305:1425–1430, 1981Google Scholar
  3. 3.
    Siegel FP, Lopez C, Hammer GS,et al.: Severe acquired immunodeficiency in male homosexuals manifested by chronic perianal ulcerative herpes simplex lesions. N Engl J Med 305:1439–1444, 1981Google Scholar
  4. 4.
    Viera J, Frank E, Spira TJ, Landesman SH: Acquired immune deficiency in Haitians. N Engl J Med 308:125–129, 1983Google Scholar
  5. 5.
    Rook AH, Masur H, Lane HC,et al.: Interleukin-2 enhances the depressed natural killer and cytomegalovirus-specific cytotoxic activities of lymphocytes from patients with the acquired immune deficiency syndrome. J Clin Invest 72:398–403, 1983Google Scholar
  6. 6.
    Lane HC, Masur H, Edgar LC, Whalen G, Rook AH, Fauci AS: Abnormalities of B-cell activation and immunoregulation in patients with the acquired immune deficiency syndrome. N Engl J Med 309:453–458, 1983Google Scholar
  7. 7.
    Metroka CE, Cunningham-Rundles F, Pollack MS, Sonnabend JA, Davis JM, Gordon B, Fernandez RD, Mouradian J: Generalized lymphadenopathy in homosexual males. Ann Intern Med 99:585–591, 1983Google Scholar
  8. 8.
    Yoshida T: Lymphokines mediate and regulatein vivo delayed hypersensitivity reactions.In Advances in Immunopharmacology, JW Haddon, L Chedid, P Dukor, F Spearfico, D Willoughby (eds). New York, Pergamon Press, 1983, pp 609–614Google Scholar
  9. 9.
    Sheridan JF, Donnenberg AD, Aurelian L, Elpern DJ: Immunity to herpes simplex virus type 2. IV. Impaired lymphokine production during recrudescence correlates with an imbalance in T lymphocyte subsets. J Immunol 129:326–331, 1982Google Scholar
  10. 10.
    Irmiere A, Gibson DW: Isolation and characterization of a noninfectious virion-like particle released from cells infected with human strains of cytomegalovirus. Virology 130:118–133, 1983Google Scholar
  11. 11.
    Lachmann L: Human interleukin 1: purification and properties. Fed Proc 42:2639–2645, 1983Google Scholar
  12. 12.
    Rabin H, Hopkins RF III, Ruscetti FW, Neubauer RH, Brown RL, Kawakami TG: Spontaneous release of a factor with properties of T cell growth factor from a continuous line of primate tumor T cells. J Immunol 127:1852–1856, 1981Google Scholar
  13. 13.
    Donnenberg AD, Elfenbein GJ, Santos GW: Cell-cell cooperation in lymphocyte colony formation: Studies in human allogeneic marrow transplantation. J Immunol 129:1080–1083, 1982Google Scholar
  14. 14.
    Stahl RE, Friedman-Kien A, Dubin R, Marmov M, Zolla-Pazner S: Immunologic abnormalities in homosexual men—relationship to Kaposi's sarcoma. Am J Med 73:171–178, 1982Google Scholar
  15. 15.
    Kern DE, Gillis S, Okada M, Henney CS: The role of interleukin-2 (IL-2) in the differentiation of cytotoxic T cells: The effect of monoclonal anti-IL-2 antibody and absorption with IL-2 dependent T cell lines. J Immunol 127:1323–1328, 1981Google Scholar
  16. 16.
    Watson J, Gorden LA, Lefkovits I: The purification and quantification of helper T cell-replacing factors secreted by murine spleen cells activated by concanavalin A. J Immunol 122:209–214, 1979Google Scholar
  17. 17.
    Iwasaka T, Sheridan JF, Aurelian L: Immunity to herpes simplex virus type 2: Recurrent lesions are associated with the induction of suppressor cells and soluble suppressor factors. Infect Immun 42:135–144, 1983Google Scholar
  18. 18.
    Reinherz EL, O'Brien C, Rosenthal P, Schlossman SF: The cellular basis for viral-induced immunodeficiency: analysis by monoclonal antibody. J Immunol 125:1269–1274, 1980Google Scholar
  19. 19.
    Mizel SB: Interleukin 1 and T cell activation. Immunol Rev 63:51–72, 1982Google Scholar
  20. 20.
    Smith KA, Gilbride KJ, Favata MF: Lymphocyte activating factor promotes T cell growth factor production by cloned murine lymphoma cells. Nature 287:353, 1980Google Scholar
  21. 21.
    Lutz CT, Glasebrook AL, Fitch FW: Alloreactive cloned T cell lines. III. Accessory cell requirements for the growth of cloned cytolytic T-lymphocytes. J Immunol 126:1404–1408, 1981Google Scholar

Copyright information

© Plenum Publishing Corporation 1984

Authors and Affiliations

  • J. F. Sheridan
    • 1
    • 3
  • L. Aurelian
    • 1
    • 2
    • 3
  • A. D. Donnenberg
    • 4
  • T. C. Quinn
    • 5
  1. 1.Division of Comparative MedicineThe Johns Hopkins Medical InstitutionsBaltimore
  2. 2.Department of Biochemistry and BiophysicsThe Johns Hopkins Medical InstitutionsBaltimore
  3. 3.The STD Research UnitThe Johns Hopkins Medical InstitutionsBaltimore
  4. 4.Bone Marrow Transplantation UnitThe Johns Hopkins Medical InstitutionsBaltimore
  5. 5.Division of Infectious DiseasesThe Johns Hopkins Medical InstitutionsBaltimore

Personalised recommendations