Journal of Clinical Immunology

, Volume 4, Issue 4, pp 287–294 | Cite as

T-cell regulation of IgG subclass expression by mitogen-induced plasma cells: Soluble factors versus the T cells

  • M. Mayumi
  • T. Kuritani
  • M. D. Cooper
Original Articles


The human IgG subclasses expressed by plasma cells generated from circulating B cells in response to soluble T-cell factors were examined by immunofluorescence using subclass-specific monoclonal antibodies. Soluble T-cell factors were induced by a mixed lymphocyte reaction, pokeweed mitogen (PWM), or phytohemagglutinin. The distribution of IgG subclasses expressed by plasma cells induced by these factors was IgG2> 5%, IgG1<25%, IgG3<1%, and IgG4<1%. On the other hand, IgG1 was dominant when B cells were cultured with T cells and PWM: IgG1 ∼70%, IgG2 ∼20%, IgG3 ∼8%, and IgG4 ∼1%. The addition of different amounts of the T-cell factors to B cells in culture did not alter the predominance of IgG2 plasma-cell differentiation. These results suggest that T cells and their soluble factors may preferentially enhance terminal differentiation of different IgG B-cell subpopulations. In contrast, the ratio of IgA1 to IgA2 plasma-cell responses was ∼1.5 to 1 regardless of whether the B-cell precursors were induced by T-cell factors or by the T cells plus PWM.

Key words

IgG surfaces regulation factors mitogens 


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Copyright information

© Plenum Publishing Corporation 1984

Authors and Affiliations

  • M. Mayumi
    • 1
  • T. Kuritani
    • 1
  • M. D. Cooper
    • 1
  1. 1.The Cellular Immunobiology Unit of the Tumor Institute, Department of Pediatrics and Microbiology, and The Comprehensive Cancer CenterUniversity of Alabama in BirminghamBirmingham

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