Abstract
We have used a panel of monoclonal antibodies to examine the development of lymphoid and myeloid sub-populations of cells in thymus, bone marrow, and liver of 16 fetuses from 12 to 16 weeks of gestational age. Pre-B and IgM+ B cells were present at a ratio of approximately 2:1 in all of the fetal bone marrow and liver samples; cells of both phenotypes were HLA-DR+ but did not express the mature B-cell antigen, HB-2. Cells expressing the myelomonocytic antigen, MMA or Leu-M1, were more frequent in bone marrow (40%) than in fetal liver (10%), and cells expressing the HNK-1 or Leu-7 antigen were rare (<1%) in all of the fetal tissues examined. Each of the T-cell antigens, T1, 4, 5, 6, and 8, was expressed by a majority of thymocytes irrespective of the age of the fetal donor. In contrast, cells with the T1, 4, 5, and 8 antigens were not seen in bone marrow and liver before the 13th week of gestation, and T6+ cells were never seen in these hemopoietic tissues. These results suggest that fetal liver and bone marrow precursors in humans do not express these T-cell antigens prior to thymic entry and the onset of thymocyte differentiation.
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Kamps, W.A., Cooper, M.D. Development of lymphocyte subpopulations identified by monoclonal antibodies in human fetuses. J Clin Immunol 4, 36–39 (1984). https://doi.org/10.1007/BF00915285
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DOI: https://doi.org/10.1007/BF00915285