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Production of anti-basement membrane-zone antibody by peripheral blood leukocytes of bullous pemphigoid patients

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Abstract

Peripheral blood leukocytes (PBL) from patients with bullous pemphigoid stimulated by pokeweed mitogen (PWM) produce an anti-basement membrane-zone (BMZ) antibody. The antibody producedin vitro was biosynthetically radiolabeled, and its binding to the BMZ was histologically demonstrated by autoradiography. The IgG nature of the antibody was evidenced by the fact that it was absorbed by rabbit anti-human IgG antiserum immunoabsorbents. The peak antibody productionin vitro occurred after 6 days of culture. Antibody producedin vitro did not bind to the BMZ of human skin, but it did bind to the BMZ of the monkey esophagus. Normal human volunteers and control patients did not produce such an antibody. In other experiments the effects of adding serum containing anti-BMZ antibody to the synthesis of Ig by cells of patients and normal subjects were studied. Although serum from patients with bullous pemphigoid stimulated Ig production with either normal or BP-patient PBL, the difference was not statistically significant. The availability of the assay system allows for studying the immunoregulatory mechanism of autoantibody production in bullous pemphigoid.

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References

  1. Lever WF: Pemphigus. Medicine (Baltimore) 32:1–23, 1953

    Google Scholar 

  2. Eng AM, Moncada B: Bullous pemphigoid and dermatitis herpetiformis, histopathologic differentiation of bullous pemphigoid and dermatitis herpetiformis. Arch Dermatol 110:51–57, 1978

    Google Scholar 

  3. Lever WF: Pemphigus and pemphigoid. J Acad Dermatol 1:2–30, 1979

    Google Scholar 

  4. Pearson JR, Rogers RS III: Bullous and cicatricial pemphigoid, clinical histopathologic, and immunopathologic correlations. Mayo Clin Proc 52:56–66, 1974

    Google Scholar 

  5. Sams WM Jr, Schur PH: Studies of the antibodies in pemphigoid and pemphigus. J Lab Clin Med 82:249–254, 1973

    Google Scholar 

  6. Sams WM, Jordon RE: Correlation of pemphigoid and pemphigus antibody with disease. Br J Dermatol 34:7–13, 1971

    Google Scholar 

  7. Jordon RE, Beutner EH, Witebsky E: Basement zone antibodies in bullous pemphigoid. JAMA 200:751–756, 1967

    Google Scholar 

  8. Ahmed AR, Maize JC, Provost TT: Bullous pemphigoid clinical and immunological follow-up after successful therapy. Arch Dermatol 113:1043–1046, 1977

    Google Scholar 

  9. Provost TT, Tomasi TB Jr: Immunopathology of bullous pemphigoid. Clin Exp Immunol 18:193–200, 1974

    Google Scholar 

  10. Laundry M, Sams WM Jr, Jordon RE: Bullous pemphigoid: Elution of in vivo fixed antibody. J Invest Dermatol 61:345–354, 1973

    Google Scholar 

  11. Jordon RE: Complement activation in bullous skin diseases. J Invest Dermatol 65:162–169, 1975

    Google Scholar 

  12. Jordon RE, Day NK, Sams WM Jr, Good RA: The complement system in bullous pemphigoid. I. Complement and component levels in sera and blister fluids. J Clin Invest 52:1207–1114, 1973

    Google Scholar 

  13. Schaumburg-Lever G, Rule A, Schmidt-Ullrich B, Lever WF: Ultrastructural localization of in vivo bound immunoglobulins in bullous pemphigoid. J Invest Dermatol 64:47–49, 1975

    Google Scholar 

  14. Schmidt-Ullrich B, Rule A, Schaumburg-Lever G, Leblanc C: Ultrastructural localization of in vivo bound complement in bullous pemphigoid. J Invest Dermatol 65:217–219, 1975

    Google Scholar 

  15. Holabar K, Wolff K, Konrad K, Beutner ER: Ultrastructural localization of immunoglobulins in bullous pemphigoid skin. J Invest Dermatol 64:220–227, 1975

    Google Scholar 

  16. Sams WM Jr, Gleich GJ: Failure to transfer bullous pemphigoid with serum from patients. Proc Soc Exp Biol Med 136:1027–1031, 1971

    Google Scholar 

  17. Binder WL, Beutner EH, Chorzelski TP: In vitro effects of pemphigus antibodies on skin. Br J Dermatol 99:39–42, 1978

    Google Scholar 

  18. Saxon A, Stevens RH, Ashman RF: REgulation of immunoglobulin production in human peripheral blood leukocytes: Cellular interactions. J Immunol 118:1872–1879, 1977

    Google Scholar 

  19. Kessler SW: Rapid isolation of antigens from cells with a staph protein A antibody absorbent: Parametres of the interaction of antibody antigen complexes with protein A. J Immunol 115:1617, 1975

    Google Scholar 

  20. Bonner WM, RA Laskey: A film detection method for labelled proteins and nucleic acids in polyacrlymide gels. Eur J Biochem 46:83, 1974

    Google Scholar 

  21. Van Furth R, Schmit HRE: Humans with formation of immunoglobulin by human tissues in vitro; Circulating lymphocytes in normal human and pathological conditions. Immunology 11:29–40, 1966

    Google Scholar 

  22. Abdou NI, Sagawa A, Pascual E, Herbert J, Sadeghee S: Suppressor T cell abnormality in idiopathic SLE. Clin Immunol Immunopathol 6:192–199, 1976

    Google Scholar 

  23. Twomey JJ, Laughter AH, Steinberg AD: A serum inhibitor of immune regulation in patients with SLE. J Clin Invest 61:713–715, 1978

    Google Scholar 

  24. Shore A, Limatibul D, Doscia HM, Gelfand EW: Identification of two serum components regulating the expression of T lymphocytes in children with myasthenia gravis. N Engl J Med 201:625–629, 1979

    Google Scholar 

  25. Moretta J, Mingari MC, Romanzi CA: Loss of Fc receptors for IgG from human T lymphocytes exposed to IgG immune complexes. Nature (London) 272:618–620, 1978

    Google Scholar 

  26. Stevens RH, Theile CJ, Saxon AS: The production of soluble T lymphocyte derived factor which substitutes for helper T lymphocytes in the in vitro production of immunoglobulin. Immunology 36:407–413, 1979

    Google Scholar 

  27. Saxon AS, Stevens RH: Suppression of immunoglobulin production in normal human blood: Characterization of the cells responsible and mediation by a soluble T lymphocyte derived factor. Clin Immunol Immunopathol 10:427–437, 1978

    Google Scholar 

  28. Sarne T, Steinberg AD, Patton-Reeves J, Green T: Studies on immune functions with SLE T cells subsets and antibodies to T cell subsets. J Clin Invest 64:1260–1263, 1979

    Google Scholar 

  29. Bystryn JC, Graf MN, Uhr JW: Regulation of antibody formation by serum antibody. II. Removal of specific antibody by means of exchange transfusion. J Exp. Med 132:1279–1282, 1970

    Google Scholar 

  30. Grawtham WC, Fitch FW: Role of antibody feedback inhibition in the regulation of secondary antibody response after high and low dose priming. J Immunol 114:394–398, 1975

    Google Scholar 

  31. Stevens RH, Saxon A: Immunoregulation in humans: Control of anti-tetanus antibody production after booster immunization. J Clin Invest 62:1156–1160, 1978

    Google Scholar 

  32. Stevens RH, Masey E, Morrow C, Saxon A: Characterization of a subpopulation of anti-tetanus toxoid antibody producing B cell population after booster immunization. J Immunol 122:2498–2509, 1979

    Google Scholar 

  33. Makela D: Single lymph node cells producing hetroclitic bacteriophage antibody. J Immunol 95:378–386, 1965

    Google Scholar 

  34. Makelo D, Karjalainen: Origins of lymphocyte diversity. Cold Spring Harbor Symp Quant Biol 41:735–741, 1977

    Google Scholar 

  35. Feibelman C, Stolzner G, Provost TT: Pemphigus vulgaris superior sensitivity of monkey esophagus in the determination of pemphigus antibody. Arch Dermatol 117:561–562, 1981

    Google Scholar 

  36. Judd KP, Mescon H: Comparison of different epithelial substrates useful for indirect immunofluorescence testing of sera from patients with active pemphigus. J Invest Dermatol 72:314–316, 1979

    Google Scholar 

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Authors and Affiliations

  1. Divisions of Dermatology and Clinical Immunology & Allergy, Department of Medicine, and Department of Microbiology & Immunology, School of Medicine, University of California, Los Angeles, 90024, Los Angeles, California

    A. Razzaque Ahmed, Ronald H. Stevens & Andrew Saxon

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  1. A. Razzaque Ahmed
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  2. Ronald H. Stevens
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  3. Andrew Saxon
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Ahmed, A.R., Stevens, R.H. & Saxon, A. Production of anti-basement membrane-zone antibody by peripheral blood leukocytes of bullous pemphigoid patients. J Clin Immunol 2, 179–185 (1982). https://doi.org/10.1007/BF00915220

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  • DOI: https://doi.org/10.1007/BF00915220

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