, Volume 13, Issue 3, pp 259–266 | Cite as

Macrophage-produced oxygen radical generating activities for polymorphonuclear leukocytes

  • Yoshio Tsukamoto
  • Sachiko Fukutani
  • Masahide Nakatani
  • Masakazu Mori
Original Articles


Guinea pig peritoneal macrophages were stimulated in vitro by bacterial lipopolysaccharide. After incubation, the supernatants of macrophage cultures were collected and tested for O 2 production on guinea pig peritoneal polymorphonuclear leukocytes. The supernatants of macrophage cultures stimulated by lipopolysaccharide had significantly higher levels of O 2 -generating activities in polymorphonuclear leukocytes, and these activities appeared in the macrophage cultures within 2 h after stimulation by lipopolysaccharide However, the supernatants obtained from the nonstimulated cultures could not produce these activities. These activities disappeared with heating or trypsin and were not produced in macrophage cultures by incubation with cycloheximide.


Oxygen Public Health Internal Medicine Trypsin Generate Activity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Plenum Publishing Corporation 1989

Authors and Affiliations

  • Yoshio Tsukamoto
    • 1
  • Sachiko Fukutani
    • 1
  • Masahide Nakatani
    • 1
  • Masakazu Mori
    • 1
  1. 1.Department of PharmacologyOsaka Dental UniversityOsakaJapan

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