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Inflammation

, Volume 12, Issue 6, pp 575–584 | Cite as

Stimulation of guinea pig neutrophil superoxide anion-producing system with thymol

  • Yukio Suzuki
  • Hiroaki Furuta
Original Articles

Abstract

Thymol stimulated O 2 production in guinea pig neutrophils. O 2 production occurred about 30 sec after the addition of thymol, and its rate was independent of extracellular Ca2 +. Thymol-induced activity was inhibited by trifluoperazine (TFP), an inhibitor of protein kinase c, and its IC50 was less than that for 12-O-tetradecanoyl phorbol 13-acetate (TPA) induced activity. After complete activation, O 2 production was reversed by addition of TFP or by washing out and resuspending in a stimuli-free medium. The responsiveness of the thymol-pulsed cells to another stimulus, TPA, was somewhat more than resting cells, but the responsiveness of the former cells to thymol was about half that of the latter cells. The ATP level of cells was reduced to one half its initial value during activation by thymol. These data suggest that the magnitude of thymol-induced O 2 production in neutrophils is dependent on the initial density of the binding sites of the cells with thymol and the initial intracellular ATP concentration.

Keywords

Public Health Internal Medicine Protein Kinase Superoxide Phorbol 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Publishing Corporation 1988

Authors and Affiliations

  • Yukio Suzuki
    • 1
  • Hiroaki Furuta
    • 1
  1. 1.Department of PharmacologyOkayama University Dental SchoolOkayamaJapan

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