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Lipoteichoic acid-antilipoteichoic acid complexes induce superoxide generation by human neutrophils

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Abstract

Human neutrophils (PMNs) which have been incubated with lipoteichoic acid (LTA) from group A streptococci generated large amounts of Superoxide (O −2 chemiluminescence and hydrogen peroxide when challenged with anti-LTA antibodies. Cytochalasin B further enhanced O *2 generation. The onset of Of generation by the LTA-anti-LTA complexes was much faster than that induced by BSA-anti-BSA complexes. LTA-treated PMNs generated much less O *2 when challenged with BSA complexes, suggesting that LTA might have blocked, nonspecifically, some of the Fc receptors on PMNs. PMNs treated with LTA-anti-LTA complexes further interacted with bystander nonsensitized PMNs resulting in enhanced Of generation, suggesting that small numbers of LTA-sensitized PMNs might recruit additional PMNs to participate in the generation of toxic oxygen species. Protelolytic enzyme treatment of PMNs further enhanced the generation of O −2 by PMNs treated with LTA-anti-LTA. Superoxide generation could also be induced when PMNs and anti-LTA antibodies interacted with target cells (fibroblasts, epithelial cells) pretreated with LTA. This effect was also further enhanced by pretreatment of the target cells with proteases. PMNs incubated with LTA released lysosomal enzymes following treatment with anti-LTA antibodies. The amounts of phosphatase,Β-glucoronidase,N-acetylglucosaminidase, mannosidase, and lysozyme release by LTA-anti-LTA complexes were much smaller than those released by antibody or histone-opsonized streptococci, suggesting that opsonized particles are more efficient lysosomal enzyme releasers. However, since the amounts of O −2 generated by the LTA complexes equaled those generated by the opsonized particles, it is assumed that the signals for triggering a respiratory burst and lysosomal enzyme secretion might be different.

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Authors and Affiliations

  1. Department of Oral Biology, Hebrew University Hadassah School of Dental Medicine Founded by Alpha Omega Fraternity, Jerusalem, Israel

    Isaac Ginsburg (Visiting Professor)

  2. Department of Pathology, The University of Michigan Medical School, Ann Arbor, Michigan

    Suzanne E. G. Fligiel, Peter A. Ward & James Varani

  3. The Department of Pathology, The University of Michigan, USA

    Isaac Ginsburg (Visiting Professor)

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  1. Isaac Ginsburg
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Supported by a research grant from Dr. S. M. Robbins of Cleveland Ohio, and by grants HL-288442, HL-31963 and GM-29507, from the National Institutes of Health, Bethesda, Maryland, and by grant IM-432 from the American Cancer Society.

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Ginsburg, I., Fligiel, S.E.G., Ward, P.A. et al. Lipoteichoic acid-antilipoteichoic acid complexes induce superoxide generation by human neutrophils. Inflammation 12, 525–548 (1988). https://doi.org/10.1007/BF00914316

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