Inflammation

, Volume 14, Issue 5, pp 471–483 | Cite as

Antiinflammatory reactivity of copper(I)-thionein

  • Ralf Miesel
  • Hans -Jürgen Hartmann
  • Ulrich Weser
Original Articles

Abstract

In unseparated human blood the reactivity of yeast copper (I)-thionein on TPA-activated polymorphonuclear leukocytes was evaluated and compared with low Mr copper chelates exerting Cu2Zn2 superoxide dismutase mimetic activity. Cu, 18ΜM, in the form of Cu-thionein was sufficient to inhibit the Superoxide production of activated human blood phagocytes by 50%. Furthermore, the scavenging of hydroxyl radicals and singlet oxygen by Cu(I)-thionein was determined, using the 2-deoxyribose fragmentation assay induced by decaying K3CrO8 and the NADPH oxidation caused by UVA illuminated psoralen, respectively. The inhibitory reactivity of Cu-thionein in both assays was compared with that of serum proteins including albumin, ceruloplasmin, transferrin, and ferritin. The galactosamine/endotoxininduced hepatitis in male NMRI mice was used to evaluate the antiinflammatory reactivity of Cu-thionein in vivo. The serum copper, superoxide dismutase, and sorbitol dehydrogenase concentrations, as well as the activity of polymorphonuclear leukocytes in unseparated blood seemed most appropriate to quantify the protective capacity of Cu-thionein in the course of an oxidative stress-dependent liver injury. The intraperitoneal application of 32.5 Μmol/kg thionein-Cu limited this damage to 45%.

Keywords

Ferritin Polymorphonuclear Leukocyte Psoralen Serum Copper NMRI Mouse 

Abbreviations

SOD

Cu2Zn2 superoxide dismutase (EC 1.15.1.1)

PMN

polymorphonuclear leukocyte

TPA

12-O-tetradecanoylphorbol-13-acetate

CuPu(Py)2

[N,N′-bis(2-pyridylmethylene-1,4-butanediamine](N,N′, N″, N″)]-copper(II)

CuPu(Im)2

[1,8-di(2-imidazolyl)-2, 7-diazaoctadiene-1, 7]-(N′,N″,N″)-copper(II), Cu(Sal)2, copper salicylate

TBA

2-thiobarbituric acid

DTPA

diethylenetriaminepentaacetic acid

SDH

sorbitol dehydrogenase (EC 1.1.1.14)

BSA

bovine serum albumin

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Copyright information

© Plenum Publishing Corporation 1990

Authors and Affiliations

  • Ralf Miesel
    • 1
  • Hans -Jürgen Hartmann
    • 1
  • Ulrich Weser
    • 1
  1. 1.Anorganische Biochemie Physiologisch-Chemisches Institut der UniversitÄt TübingenTübingenFederal Republic of Germany

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