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Inflammation

, Volume 5, Issue 2, pp 153–164 | Cite as

Effect of adrenalectomy on cadmium- and turpentine-induced hepatic synthesis of metallothionein and α2-macrofetoprotein in the rat

  • P. Z. Sobocinski
  • W. J. CanterburyJr.
  • G. L. Knutsen
  • E. C. Hauer
Original Articles

Abstract

Recent reports have strongly implicated glucocorticoids in the induction of hepatic metallothionein synthesis and hypozincemia which occurs in certain pathophysiologic conditions. Studies were performed in rats to determine the effect of adrenalectomy and glucocorticoid treatment on the hepatic accumulation of metallothionein subsequent to the administration of cadmium and turpentine, two diverse substances known to induce hypozincemia and hepatic synthesis of metallothionein as well as α2-macrofetoprotein in intact rats. By 24 h, both substances induced significant hypozincemia, hepatic metallothionein accumulation, and a severe tissue inflammatory response in adrenalectomized rats. Adrenalectomy only prevented the increase in plasma α2-macrofetoprotein concentration. Results indicate that hepatic synthesis of α2-macrofetoprotein, but not metallothionein, is mediated by adrenal hormones. Thus, glucocorticoids do not play a “vital” role in hepatic metallothionein accumulation or hypozincemia induced by inflammatory stress, as previously postulated.

Keywords

Internal Medicine Cadmium Inflammatory Response Glucocorticoid Pathophysiologic Condition 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Kojima, Y., andJ. H. R. Kägi. 1978. Metallothionein.Trends Biochem. Sci. 3:90–93.Google Scholar
  2. 2.
    Richards, M. P., andR. J. Cousins. 1975. Mammalian zinc homeostasis: requirement for RNA and metallothionein synthesis.Biochem. Biophys. Res. Commun. 64:1215–1223.Google Scholar
  3. 3.
    Sobocinski, P. Z., W. J. Canterbury, Jr., C. A. Mapes, andR. E. Dinterman. 1978. Involvement of hepatic metallothioneins in hypozincemia associated with bacterial infection.Am. J. Physiol. 234:E399-E406.Google Scholar
  4. 4.
    Sobocinski, P. Z., W. J. Canterbury, Jr., E. C. Hauer, andF. A. Beall. 1979. Induction of hypozincemia and hepatic metallothionein synthesis in hypersensitivity reactions.Proc. Soc. Exp. Biol. Med. 160:175–179.Google Scholar
  5. 5.
    Sobocinski, P. Z., G. L. Knutsen, W. J. Canterbury, Jr., andE. C. Hauer. 1979. Altered zinc homeostasis and hepatic accumulation of metallothionein in indomethacininduced enteropathy.Toxicol. Appl. Pharmacol. 50:557–564.Google Scholar
  6. 6.
    Oh, S. J., J. T. Deagen, P. D. Whanger, andP. H. Weswig. 1978. Biological function of metallothionein. V. Its induction in rats by various stresses.Am. J. Physiol. 234:E282-E285.Google Scholar
  7. 7.
    Squibb, K. S., R. J. Cousins, andS. L. Feldman. 1977. Control of zinc-thionein synthesis in rat liver.Biochem. J. 164:223–228.Google Scholar
  8. 8.
    Panemangalore, M., andF. O. Brady. 1978. Induction and synthesis of metallothionein in isolated rat liver.J. Biol. Chem. 23:7898–7904.Google Scholar
  9. 9.
    Karin, M., H. R. Herschman, andD. Weinstein. 1980. Primary induction of metallothionein by dexamethasone in cultured rat hepatocytes.Biochem. Biophys. Res. Commun. 92:1052–1059.Google Scholar
  10. 10.
    Etzel, K. R., S. G. Shapiro, andR. J. Cousins. 1979. Regulation of liver metallothionein and plasma zinc by the glucocorticoid dexamethasone.Biochem. Biophys. Res. Commun. 89:1120–1126.Google Scholar
  11. 11.
    Failla, M. L., andR. J. Cousins. 1978. Zinc accumulation and metabolism in primary cultures of adult rat liver cells. Regulation by glucocorticoids.Biochim. Biophys. Acta 543:293–304.Google Scholar
  12. 12.
    Karin, M., andH. R. Herschman. 1979. Dexamethasone stimulation of metallothionein synthesis in HeLa cell cultures.Science 204:176–177.Google Scholar
  13. 13.
    Schutz, G., M. Beato, andP. Feigelson. 1973. Messenger RNA for hepatic tryptophan oxygenase: Its partial purification, its translation in a heterologous cell-free system, and its control by glucocorticoid hormones.Proc. Natl. Acad. Sci. U.S.A. 70:1218–1221.Google Scholar
  14. 14.
    Weimer, H. E., andD. C. Benjamin. 1966. Influence of adrenal cortex on synthesis of α2-AP globulin of rat serum.Proc. Soc. Exp. Biol. Med. 122:1112–1114.Google Scholar
  15. 15.
    Failla, M. L., andR. J. Cousins. 1978. Zinc uptake by isolated rat liver parenchymal cells.Biochim. Biophys. Acta 538:435–444.Google Scholar
  16. 16.
    Flynn, A., W. J. Pories, W. H. Strain, O. A. Hill, Jr., andR. B. Fratianne. 1971. Rapid serum-zinc depletion associated with corticosteroid therapy.Lancet 2:1169–1172.Google Scholar
  17. 17.
    Sobocinski, P. Z., W. J. Canterbury, Jr., C. A. Mapes, R. E. Dinterman, E. C. Hauer, andF. B. Abeles. 1978. Hypozincemia of inflammation: sequestration of zinc by hepatic metallothioneins.Fed. Proc. 37:809.Google Scholar
  18. 18.
    Bostian, K. A., B. S. Blackburn, R. W. Wannemacher, Jr., V. G. McGann, W. R. Beisel, andH. L. Dupont. 1976. Sequential changes in the concentration of specific serum proteins during typhoid fever infection in man.J. Lab. Clin. Med. 87:577–585.Google Scholar
  19. 19.
    Beisel, W. R., R. S. Pekarek, andR. W. Wannemacher, Jr. 1976. Homeostatic mechanisms affecting plasma zinc levels in acute stress.In Trace Elements in Human Health and Disease, Vol. 1. A. S. Prasad, editor. Academic Press, New York. 87–106.Google Scholar
  20. 20.
    Sobocinski, P. Z., W. J. Canterbury, Jr., andC. A. Mapes. 1977. Induction of hepatic zinc-binding proteins by endotoxin and leucocytic endogenous mediators (LEM).Fed. Proc. 36:1100.Google Scholar
  21. 21.
    Thompson, W. L., F. B. Abeles, F. A. Beall, R. E. Dinterman, andR. W. WanneMacher, Jr. 1976. Influence of the adrenal glucocorticoids on the stimulation of synthesis of hepatic ribonucleic acid and plasma acute-phase globulins by leucocytic endogenous mediator.Kochern. J. 156:25–32.Google Scholar
  22. 22.
    Oh, S. H., J. T. Deagen, P. D. Whanger, andP. H. Weswig. 1978. Biological function of metallothionein—IV. Biosynthesis and degradation of liver and kidney metallothionein in rats fed diets containing zinc or cadmium.Bioinorg. Chem. 8:245–254.Google Scholar
  23. 23.
    Kotsonis, F. N., andC. D. Klaassen. 1979. Increase in hepatic metallothionein in rats treated with alkylating agents.Toxicol. Appl. Pharmacol. 51:19–27.Google Scholar
  24. 24.
    Shapiro, S. G., K. S. Squibb, L. A. Markowitz, andR. J. Cousins. 1978. Cell-free synthesis of metallothionein directed by rat liver polyadenylated messenger ribonucleic acid.Biochem. J. 175:833–840.Google Scholar

Copyright information

© Plenum Publishing Corporation 1981

Authors and Affiliations

  • P. Z. Sobocinski
    • 1
  • W. J. CanterburyJr.
    • 1
  • G. L. Knutsen
    • 1
  • E. C. Hauer
    • 1
  1. 1.U.S. Army Medical Research Institute of Infectious Diseases Fort DetrickFrederick

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