Inflammation

, Volume 14, Issue 4, pp 409–419 | Cite as

Reactivity of active center analogs of Cu2Zn2 superoxide dismutase on activated polymorphonuclear leukocytes

  • Ralf Miesel
  • Hans-Jürgen Hartmann
  • Yuejin Li
  • Ulrich Weser
Original Articles

Abstract

In unseparated human blood the Cu2Zn2 superoxide dismutase mimetic reactivity of several differently coordinated low Mr copper chelates on TPA-activated polymorphonuclear leukocytes was evaluated and compared to their apo-chelates, CuSO4, and the native enzyme. Similar to intact superoxide dismutase, 350–400 nM Cu flexibly complexed in a di-Schiff base mode in CuPu(Py)2 and CuPu(Im)2, respectively, was sufficient to inhibit the oxidative burst-dependent superoxide production of human blood phagocytes by 50%. Acetate-or biuret-type copper chelates behaved like CuSO2. The catalytic superoxide dismuting reactivity of the di-Schiff base active center analogs of SOD was confirmed using isolated porcine PMNs. Even in the presence of 600μM albumin as a model for competitive copper chelation in biological fluids CuPu(Py)2 and CuPu(Im)2 remained active. The stability during the Cu(I)/Cu(II) redox cycling was demonstrated in the presence of activated PMNs and albumin, taking advantage of the electron paramagnetic properties of CuPu(Py)2 and CuPu(Im)2.

Keywords

Albumin Superoxide Superoxide Dismutase Human Blood CuSO4 

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Copyright information

© Plenum Publishing Corporation 1990

Authors and Affiliations

  • Ralf Miesel
    • 1
  • Hans-Jürgen Hartmann
    • 1
  • Yuejin Li
    • 1
  • Ulrich Weser
    • 1
  1. 1.Anorganische Biochemie Physiologisch-Chemisches InstitutUniversität TübingenTübingenF.R.G.

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