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Inflammation

, Volume 3, Issue 2, pp 129–135 | Cite as

Rapid micromeasurement of neutrophil exocytosis

  • Robert O. Webster
  • Peter M. Henson
Article

Abstract

A simple method is described for the visual detection of neutrophil granule exocytosis. The microassay measures release of myeloperoxidase from azurophilic granules following neutrophil stimulation and can be accomplished in less than 30 min. The assay is sensitive, reproducible from day to day, and does not require separation of leukocytes from the reaction before enzyme assay. The procedure is also applicable to the rapid measurement of the suppression of degranulation by antiinflammatory agents.

Keywords

Public Health Internal Medicine Enzyme Assay Antiinflammatory Agent Visual Detection 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Publishing Corporation 1979

Authors and Affiliations

  • Robert O. Webster
    • 1
  • Peter M. Henson
    • 1
  1. 1.Department of PediatricsNational Jewish Hospital and Research Center and University of Colorado School of MedicineDenver

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