, Volume 6, Issue 1, pp 31–38 | Cite as

Modulation of human lymphocyte transformation by bacterial products and leukocyte lysates

  • M. N. Sela
  • I. Ginsburg
  • T. Dishon
  • Z. Duchan
  • A. A. Garfunkel
Original Articles


Blast transformation of human peripheral blood lymphocytes by PHA is shown to be modulated by lipoteichoic acid (LTA) ofStreptococcus mutans, by a cell-sensitizing factor ofActinomyces viscosus, as well as by a frozen and thawed extract of human leukocytes (LE). While small amounts of LE (5–50Μg/106 cells) significantly enhanced PHA-induced transformation, higher amounts showed a lesser effect on the blastogenic response. Both LTA and theA. viscosus extract did not cause any lymphocyte blastogenic effect when used alone. On the other hand LTA had an inhibitory effect and theA. viscosus extract had an enhancing effect when lymphocytes were pretreated by these agents and then exposed to PHA.


Public Health Internal Medicine Blood Lymphocyte Peripheral Blood Lymphocyte Human Leukocyte 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Oppenheim, J. J. 1968. Relationship of in vitro lymphocyte transformation to delayed hypersensitivity in guinea pigs and man.Fed. Proc. 27:21.Google Scholar
  2. 2.
    Anderson, J., F. Melchers, C. Galanos, andO. Luderitz. 1973. The mitogenic effect of lipopolysaccharide on bone marrow-derived mouse lymphocytes. Lipid A as the mitogenic part of the molecule.J. Exp. Med. 137:943.Google Scholar
  3. 3.
    Gery, I., Y. Kruger, andS. Z. Spiesel. 1972. Stimulation of B lymphocytes by endotoxin.Immunology 108:1088.Google Scholar
  4. 4.
    Vischer, T. L. 1972. Immunoglobulin-like surface molecules and theta antigen during the specific and non-specific stimulations of mouse spleen cells in vitro.Clin. Exp. Immunol. 11:523.Google Scholar
  5. 5.
    Sela, M. N., M. Lahav, andI. Ginsburg. 1977. Effect of leukocyte hydrolases on bacteria. IX. The release of lipotiochic acid from group A streptococci and fromStrep. mutans by leukocyte extracts and by lysozyme: Relation to tissue damage in inflammatory sites.Inflammation 2:151.Google Scholar
  6. 6.
    Ginsburg, I. M.N. Sela, A. Morag, Z. Ravid, Z. Duchan, M. Ferne, S. Rabinowitz-Bergner, P.P. Thoma, P. Davies, J. Niccols, J. Humes, andR. Bonney. 1981. Role of leukocyte factors and cationic polyelectrolytes in phagocytosis of groups A streptocci andCandida albicans by neutrophils, macrophages, fibroblasts and epithelial cells: Modulation by anionic polyelectrolytes in relation to pathogenesis of chronic inflammation.Inflammation 5:301.Google Scholar
  7. 7.
    Ferne, M., S. Bergner-Rabinowitz, andI. Ginsburg. 1976. The effect of leukocyte hydrolases on bacteria. VI. The role played by leukocyte extracts in the sensitization of RBC by lipopolysaccharides and by the cell sensitizing factor of group A streptococci.Inflammation 1:247.Google Scholar
  8. 8.
    Ferne, M., Z. Duchan, S. Rabinowitz, M. N. Sela, andI. Ginsburg. 1978. The effect of leukocyte hydrolases on bacteria XII. The release of lipopolysaccharid (LPS) fromSalmonella typhi by leukocyte extracts, lysozyme, inflammatory exudates and by serum and synovial fluid and the modulation of anionic and cationic polyelectrolytes of LPS release and the sensitization or erythrocytes.Inflammation 3:59.Google Scholar
  9. 9.
    Moskowitz, M. 1966. Separation of properties of a red cell sensitizing substance from streptococci.J. Bacteriol. 91:2200.Google Scholar
  10. 10.
    Wicken, A. J., andK. W. Knox. 1975. Lipoteichoic acids: A new class of bacterial antigen.Science 187:1162.Google Scholar
  11. 11.
    Sela, M. N. 1978. The role played by dental plaque and its constituents on the initiation of inflammation in connective tissue. PhD thesis, Hebrew University, Jerusalem.Google Scholar
  12. 12.
    Dukor, P., andL. U. Hartman. 1973. Hypothesis. Bound C3 as the second signal for B cell activation.Cell. Immunol. 7:349.Google Scholar
  13. 13.
    Yoshinaga, M., S. Nakamura, andH. Hayashi. 1975. Interaction between lymphocytes and inflammatory exudate cells. 1. Enhancement of thymocyte response to PHA by product(S) of polymorphonuclear leukocytes and macrophages.J. Immunol. 115:533.Google Scholar
  14. 14.
    Möller, G. 1970. Induction of DNA synthesis in human lymphocytes: Interaction between non-specific mitogens and antigens.Immunology 19:583.Google Scholar

Copyright information

© Plenum Publishing Corporation 1982

Authors and Affiliations

  • M. N. Sela
    • 1
  • I. Ginsburg
    • 1
  • T. Dishon
    • 1
  • Z. Duchan
  • A. A. Garfunkel
    • 2
  1. 1.Department of Oral BiologyHebrew University-Hadassah School of Dental Medicine Founded by the Alpha Omega FraternityIsrael
  2. 2.Hospital Oral Medicine ServiceJerusalemIsrael

Personalised recommendations