Applied Microbiology and Biotechnology

, Volume 32, Issue 4, pp 380–386 | Cite as

Production of glycerol by immobilizedPichia farinosa

  • B. Bisping
  • U. Baumann
  • H. -J. Rehm


Cells of the osmophilic yeastPichia farinosa were immobilized in sintered glass Raschig rings for the production of glycerol. The kinetics of production were observed under different conditions in batch, fed-batch and semicontinuous fermentations in fixed-bed column reactors and compared with those of free cells. 2.6 × 109 cells/g sintered glass were adsorbed. The glycerol productivity amounted to 8.1 g/l per day. The highest concentration reached in batch culture was 86 g/l with immobilized cells. Fermentations using immobilized cells were accelerated compared to fermentations using free cells and maximum yield and productivity were reached at lower initial sugar concentrations. Using scanning electron microscopy it was observed that the shape of the cells was related to the sugar concentration in the medium. The experiments show thatP. farinosa produces glycerol with a high and constant productivity over long periods of time.


Sugar Microscopy Electron Microscopy Scanning Electron Microscopy Fermentation 
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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • B. Bisping
    • 1
  • U. Baumann
    • 1
  • H. -J. Rehm
    • 1
  1. 1.Institut für MikrobiologieUniversität MünsterMünsterGermany

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