Applied Microbiology and Biotechnology

, Volume 42, Issue 2–3, pp 440–445 | Cite as

Anaerobic degradation of chloroacetanilide herbicides

  • A. Konopka
Original Paper


Anaerobic microbial transformations of chloroacetanilide herbicides (alachlor, metolachlor, and propachlor) and aniline were investigated. Modest microbial transformations (in most cases 30–60% losses) of chloroacetanilide herbicides were found in agricultural soils incubated under anaerobic conditions for 30 days. Greater anaerobic losses were observed in anaerobic digestor sludge or rumen fluid. Anilines were not substantially transformed in any system within 30 days. The highest anaerobic activities metabolizing alachlor or propachlor occurred in anaerobic sludge. However, this biological activity could only be transferred if autoclaved sludge digestor material was added to the transfer medium, which suggests that the activities involved co-metabolism of the substrates.


Sludge Aniline Anaerobic Condition Agricultural Soil Anaerobic Digestor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. Attaway HH, Camper ND, Paynter MJB (1982) Anaerobic microbial degradation of diuron by pond sediment. Pestic Biochem Physiol 17:96–101Google Scholar
  2. Berry DF, Francis AJ, Bollag J-M (1987) Microbial metabolism of homocyclic and heterocyclic aromatic compounds under anaerobic conditions. Microbiol Rev 51:43–59Google Scholar
  3. Bollag J-M, McGahen LL, Minard RD, Liu SY (1986) Bioconversion of alachlor in a anaerobic stream sediment. Chemosphere 15:153–162Google Scholar
  4. Chesters G, Simsiman GV, Levy J, Alhajar BJ, Fathulla RN, Harkin JM (1989) Environmental fate of alachlor and metalachlor. Rev Environ Contam Toxicol 110:1–74Google Scholar
  5. Dangel W, Brackman R, Lack A, Mohamed M, Koch J, Oswald B, Seyfried B, Tschech A, Fuchs G (1991) Differential expression of enzyme activities initiating anoxic metabolism of various aromatic compounds via benzoyl-coenzyme A. Arch Microbiol 155:256–262Google Scholar
  6. Dolfing J, Zeyer J, Binder-Eicher P, Schwarzenbach RP (1990) Isolation and characterization of a bacterium that mineralizes tolunene in the absence of molecular oxygen. Arch Microbiol 154:336–341Google Scholar
  7. Horowitz A, Shelton DR, Cornell CP, Tiedje JM (1981) Anaerobic degradation of aromatic compounds in sediments and digested sludge. Dev Ind Microbiol 23:435–444Google Scholar
  8. Hungate RE (1979) A roll tube method for cultivation of strict anaerobes. Methods Microbiol, 3B:117–132Google Scholar
  9. Konopka A, Knight DA, Turco,RF (1989) Characterization of aPseudomonas sp. capable of aniline degradation in the presence of secondary carbon sources. Appl Environ Microbiol 55:385–389Google Scholar
  10. Kuhn EP, Suflita JM (1989a) Dehalogenation of pesticides by anaerobic microorganisms in soils and groundwater - a review. In: Sawhney BL, Brown K (eds) Reactions and movement of organic chemicals in soils (SSSA Special Publication no. 22) Soil Science Society of America, Madison, Wis., 111–180Google Scholar
  11. Kuhn EP, Suflita JM (1989b) Sequential reductive dehalogenation of chloroanilines by microorganisms from a methanogenic aquifer. Environ Sci Technol 23:648–652Google Scholar
  12. Liu S-Y, Zhang R, Bollag J-M (1988) Biodegradation of metolachlor in a soil perfusion experiment. Biol Fertil Soils 5:276–281Google Scholar
  13. Liu S-Y, Lu M-H, Bollag J-M (1990) Transformation of metolachlor in soil inoculated with aStreptomyces sp. Biodegradation 1:9–17Google Scholar
  14. Mikesell MD, Boyd SA (1985) Reductive dechlorination of the pesticides 2,4-D, 2,4,5-T, and pentachlorophenol in anaerobic sludges. J Environ Qual 14:337–340Google Scholar
  15. Pelsy F, Leroux P, Heslot H (1987) Properties of anAspergillus nidulans propanil hydrolase. Pestic Biochem Physiol 27:182–188Google Scholar
  16. Pettigrew CA, Paynter MJB, Camper ND (1985) Anaerobic microbial degradation of the herbicide propanil. Soil Biol Biochem 17:815–818Google Scholar
  17. Pothuluri JV, Moorman TB, Obenhuber DC, Wauchope RD (1990) Aerobic and anaerobic degradation of alachlor in samples from a surface-to-groundwater profile. J Environ Qual 19:525–530Google Scholar
  18. Quensen JF, Tiedje JM, Boyd SA (1988) Reductive dechlorination of polychlorinated biphenyls by anaerobic microorganisms from sediments. Science 242:752–754Google Scholar
  19. Schink B (1988) Principles and limits of anaerobic degradation: environmental and technological aspects. In: Zehnder AJB (ed) Biology of anaerobic microorganisms. Wiley, New York, pp 771–846Google Scholar
  20. Schnell S, Bak F, Pfennig N (1989) Anaerobic degradation of aniline and dihydroxybenzenes by newly isolated sulfate-reducing bacteria and description ofDesufobacterium anilini. Arch Microbiol 152:556–563Google Scholar
  21. Steep TD, Camper ND, Paynter MJB (1985) Anaerobic microbial degradation of selected 3,4-dihalogenated aromatic compounds. Pestic Biochem Physiol 23:256–260Google Scholar
  22. Struijs J, Rogers JE (1989) Reductive dehalogenation of dichloroanilines by anaerobic microorganisms in fresh and dichlorophenolacclimated pond sediment. Appl Environ Microbiol 55:2527–2531Google Scholar
  23. Tiedje JM, Sexstone AJ, Parkin TB, Revsbech NP, Shelton DR (1984) Anaerobic processes in soil. Plant Soil 76:197–212Google Scholar
  24. Villarreal DT, Turco RF, Konopka A (1991) Propachlor degradation by a soil bacterial community. Appl Environ Microbiol 57:2135–2140Google Scholar
  25. Williams PP (1977) Metabolism of synthetic organic pesticides by anaerobic microorganisms. Residue Rev 66:63–135Google Scholar

Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • A. Konopka
    • 1
  1. 1.Department of Biological SciencesPurdue UniversityWest LafayetteUSA

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