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Applied Microbiology and Biotechnology

, Volume 42, Issue 2–3, pp 432–439 | Cite as

Microcalorimetry as a diagnostic and analytical tool for the assessment of biodegradation of 2,4-D in a liquid medium and in soil

  • S. Fradette
  • D. Rho
  • R. Samson
  • A. LeDuy
Original Paper

Abstract

The biodegradation of the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) byPseudomonas cepacia was assessed by microcalorimetry in a liquid medium and in sterilized soil at 25°C under aerobic conditions. It was found that thermograms of the rate of heat evolved versus time (dQ/dt versust) can be used as a diagnostic tool to identify the timet1 required for the primary biodegradation of 2,4-D and the timetf required for the completion of the biodegradation activity in a liquid medium as well as in soil. Microcalorimetry can also be used as an analytical tool to monitor the progress of 2,4-D consumption during the biodegradation process in a liquid medium and to measure the importance of the soil sorption/desorption of intermediate metabolites. A new concept called “bioeffort” was defined as the product of the biodegradation time (t) and the biomass concentration (X) at that time. This concept was used to predict either the biomass concentration required or the duration of the primary biodegradation of 2,4-D in soil from the data obtained from a liquid medium.

Keywords

Biomass Biodegradation Liquid Medium Diagnostic Tool Analytical Tool 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • S. Fradette
    • 1
  • D. Rho
    • 2
  • R. Samson
    • 2
  • A. LeDuy
    • 1
  1. 1.Department of Chemical EngineeringLaval UniversityQuébecCanada
  2. 2.Biotechnology Research InstituteNational Research Council of CanadaMontréalCanada

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