Applied Microbiology and Biotechnology

, Volume 42, Issue 2–3, pp 432–439 | Cite as

Microcalorimetry as a diagnostic and analytical tool for the assessment of biodegradation of 2,4-D in a liquid medium and in soil

  • S. Fradette
  • D. Rho
  • R. Samson
  • A. LeDuy
Original Paper


The biodegradation of the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) byPseudomonas cepacia was assessed by microcalorimetry in a liquid medium and in sterilized soil at 25°C under aerobic conditions. It was found that thermograms of the rate of heat evolved versus time (dQ/dt versust) can be used as a diagnostic tool to identify the timet1 required for the primary biodegradation of 2,4-D and the timetf required for the completion of the biodegradation activity in a liquid medium as well as in soil. Microcalorimetry can also be used as an analytical tool to monitor the progress of 2,4-D consumption during the biodegradation process in a liquid medium and to measure the importance of the soil sorption/desorption of intermediate metabolites. A new concept called “bioeffort” was defined as the product of the biodegradation time (t) and the biomass concentration (X) at that time. This concept was used to predict either the biomass concentration required or the duration of the primary biodegradation of 2,4-D in soil from the data obtained from a liquid medium.


Biomass Biodegradation Liquid Medium Diagnostic Tool Analytical Tool 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. Baudu M, Le Cloirec P, Martin G (1993) First approach of desorption energies of water and organic molecules onto activated carbon by differential scanning calorimetry studies. Water Res 27:69–76Google Scholar
  2. Cooney CL, Wang DIC, Mateles RI (1968) Measurement of heat evolution and correlation with oxygen consumption during microbial growth. Biotechnol Bioeng 11:269–281Google Scholar
  3. Evans WC, Smith BSW, Fernley HN, Davies JI (1971) Bacterial metabolism of 2,4-dichlorophenoxyacetate. Biochem J 122:543–551Google Scholar
  4. Fradette S (1993) Comparaison de la respirométrie et de la calorimétrie comme outils d'évaluation de l'activité biologique dans les sols contaminés par l'herbicide 2,4-D. M.Sc. Thesis, Laval UniversityGoogle Scholar
  5. Greer CW, Hawari J, Samson R (1990) Influence of physicochemical factors on the degradation of 2,4-dichlorophenoxy acetic acid by aPseudomonas sp. isolated from peat moss. Arch Microbiol 154:317–322Google Scholar
  6. Ishikawa Y, Nonoyama Y, Shoda M (1981) Microcalorimetric study of aerobic growth ofEscherichia coli in batch culture. Biotechnol Bioeng 23:2825–2836Google Scholar
  7. Kawabata T, Yamano H, Takahashi K (1983) An attempt to characterize calorimetrically the inhibitory effect of foreign substances on microbial degradation of glucose in soil. Agric Biol Chem 47:1281–1288Google Scholar
  8. Kimura T, Takahashi K (1985) Calorimetric studies of soil microbes: quantitative relation between heat evolution during microbial degradation of glucose and changes in microbial activity in soil. J Gen Microbiol 131:3083–3089Google Scholar
  9. Loos MA, Roberts RN, Alexander M (1967) Formation of 2,4-dichlorophenol and 2,4-dichloroanisole from 2,4-dichlorophenoxyacetate byArthrobacter sp. Can J Microbiol 13:691–699Google Scholar
  10. Lovrien RE, Ferry ML, Magnuson TS, Blanchette RA (1989) Microbial calorimetric analysis. ACS Symp Ser 399:544–558Google Scholar
  11. Luong JHT, Volesky B (1980) Determination of the heat of some aerobic fermentations. Can J Chem Eng 58:497–504Google Scholar
  12. Radjendirane V, Bhat MA, Vaidyanathan CS (1991) Affinity purification and characterization of 2,4-diehlorophenol hydroxylase fromPseudomonas cepacia. Arch Biochem Biophysics 288:169–176Google Scholar
  13. Rochkind ML, Blackburn JW, Sayler GS (1986) Microbial decomposition of chlorinated aromatic compounds. EPA report no. EPA/600/2-86/090Google Scholar
  14. Roy D, Samson R (1988) Investigation of growth and metabolism ofSaccharomyces cerevisiae (baker's yeast) using microcalorimetry and bioluminometry. J. Biotechnol 8:193–206Google Scholar
  15. Sand W, Schröter A, Fortnagel P, Bock E (1990) Differentiation of plasmid-containingEscherichia coli strains by microcalorimetry. J Microbiol Methods 12:247–251Google Scholar
  16. Sinton GL, Fan LT, Erickson LE, Lee SM (1986) Biodegradation of 2,4-D and related xenobiotic compounds. Enzyme Microb Technol 8:395–403Google Scholar
  17. Soil Survey Staff (1975) Soil taxonomy: a basic system of soil classification for making and interpreting soil surveys. USDA-SCS Agricultural Handbook 436. U.S. Government Printing Office, Washington, D.C.Google Scholar
  18. Sparling GP (1983) Estimation of microbial biomass and activity in soil using microcalorimetry. J Soil Sci 34:381–390Google Scholar
  19. Stockar U von, Marison IN (1989) The use of calorimetry in biotechnology. Adv Biochem Eng 40:93–136Google Scholar
  20. Yamano H, Takahashi K (1983) Temperature effect on the activity of soil microbes measured from heat evolution during the degradation of several carbon sources. Agric Biol Chem 47: 1493–1499Google Scholar

Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • S. Fradette
    • 1
  • D. Rho
    • 2
  • R. Samson
    • 2
  • A. LeDuy
    • 1
  1. 1.Department of Chemical EngineeringLaval UniversityQuébecCanada
  2. 2.Biotechnology Research InstituteNational Research Council of CanadaMontréalCanada

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