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Applied Microbiology and Biotechnology

, Volume 42, Issue 2–3, pp 415–420 | Cite as

Degradation of chlorobenzenes in soil slurry by a specialized organism

  • F. R. Brunsbach
  • W. Reineke
Original Paper

Abstract

The microbial degradation of monochloro-, 1,2-dichloro-, 1,4-dichloro-, and 1,2,4-trichlorobenzene in soil slurries was examined with single compounds as well as in mixtures. The indigenous soil populations brought about the degradation of monochlorobenzene when incubated at 27°C in slurries with 29% (w/w) suspended solids. In contrast, the other chlorobenzenes persisted during an incubation period of 1 month. Supplementation with buffer, mineral salts and acetate did not significantly influence the degradation. However, inoculation withPseudomonas aeruginosa strain RHO1, a monochloro- and 1,4-dichlorobenzene-degrading organism, to a titre of 1 × 105 cells/g soil, led to rapid and complete degradation of 0.8 mm growth substrate within 30 h. In addition, the strain was able to degrade 1,2-dichloro- and 1,2,4-trichlorobenzene with stoichiometric release of chloride in the presence of acetate, ethanol, monochloro- or 1,4-dichlorobenzene as growth substrates. In mixtures of chlorobenzenes the co-metabolism of 1,2-dichloro- and 1,2,4-trichlorobenzene occurred until the growth substrates monochloroand 1,4-dichlorobenzene were degraded. The degradation was faster in the slurries of garden soil containing 8% organic carbon than in soil with the lower content of 2.6%.

Keywords

Acetate Organic Carbon Microbial Degradation Incubation Period Lower Content 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • F. R. Brunsbach
    • 1
  • W. Reineke
    • 1
  1. 1.Bergische Universität-Gesamthochschule Wuppertal, Chemische MikrobiologieWuppertalGermany

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