Applied Microbiology and Biotechnology

, Volume 42, Issue 2–3, pp 415–420 | Cite as

Degradation of chlorobenzenes in soil slurry by a specialized organism

  • F. R. Brunsbach
  • W. Reineke
Original Paper

Abstract

The microbial degradation of monochloro-, 1,2-dichloro-, 1,4-dichloro-, and 1,2,4-trichlorobenzene in soil slurries was examined with single compounds as well as in mixtures. The indigenous soil populations brought about the degradation of monochlorobenzene when incubated at 27°C in slurries with 29% (w/w) suspended solids. In contrast, the other chlorobenzenes persisted during an incubation period of 1 month. Supplementation with buffer, mineral salts and acetate did not significantly influence the degradation. However, inoculation withPseudomonas aeruginosa strain RHO1, a monochloro- and 1,4-dichlorobenzene-degrading organism, to a titre of 1 × 105 cells/g soil, led to rapid and complete degradation of 0.8 mm growth substrate within 30 h. In addition, the strain was able to degrade 1,2-dichloro- and 1,2,4-trichlorobenzene with stoichiometric release of chloride in the presence of acetate, ethanol, monochloro- or 1,4-dichlorobenzene as growth substrates. In mixtures of chlorobenzenes the co-metabolism of 1,2-dichloro- and 1,2,4-trichlorobenzene occurred until the growth substrates monochloroand 1,4-dichlorobenzene were degraded. The degradation was faster in the slurries of garden soil containing 8% organic carbon than in soil with the lower content of 2.6%.

Keywords

Acetate Organic Carbon Microbial Degradation Incubation Period Lower Content 

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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • F. R. Brunsbach
    • 1
  • W. Reineke
    • 1
  1. 1.Bergische Universität-Gesamthochschule Wuppertal, Chemische MikrobiologieWuppertalGermany

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