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Monatshefte für Chemie / Chemical Monthly

, Volume 108, Issue 1, pp 1–6 | Cite as

Die Reindarstellung von Anti-BOP-Antikörpern mittels Affinitätschromatographie

  • H. Stemberger
  • G. Wiedermann
  • W. Frisch-Niggemeyer
  • M. M. Müller
Organische Chemie und Biochemie
  • 26 Downloads

The purification of anti-BPO antibodies by means of affinity chromatograph

Abstract

The purification of anti-BPO-antibodies and anti-BPO-IgG resp. by means of affinity chromatography is being described. The immunosorbent was prepared by direct covalent coupling of BPO groups to AH-Sepharose 4B beads. The antibodies were linked to the immunosorbent in a batch and eluted by stepwise chromatography. The hapten density of the gel was very high (3.6×10−5 mole BPO/1 g Sepharose 4B) as could be demonstrated by application of14C-penicillin-potassium. Good yields of highly purified anti-PBO-antibodies were obtained.

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Copyright information

© Springer-Verlag 1977

Authors and Affiliations

  • H. Stemberger
    • 1
  • G. Wiedermann
    • 1
  • W. Frisch-Niggemeyer
    • 1
  • M. M. Müller
    • 1
  1. 1.Institut für Spezifische Prophylaxe und Tropenmedizin, Institut für Virologie und Institut für Medizinische ChemieUniversität WienWienÖsterreich

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