Summary
In two oo-type mutant strains ofE. coli K12 the mutant sites are shown to be located entirely within the structural gene of the decarboxylase component of the pyruvate dehydrogenase complex. One of the two mutant sites (probably of theochre-type) could be converted by mutation to anamber site which also completely inhibits the expression of the closely linked structural genes. The inhibition, therefore, is apparently caused by an inability to initiate, or an early termination of, the synthesis of the decarboxylase protein.
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Literatur
Alpers, D. H., andG. M. Tomkins: The order of induction and deinduction of the enzymes of the lactose operon inE. coli. Proc. nat. Acad. Sci. (Wash.)53, 797–803 (1965).
Ames, B. N., P. E. Hartman, andF. Jacob: Chromosomal alterations affecting the regulation of histidine biosynthetic enzymes inSalmonella. J. molec. Biol.7, 23–42 (1963).
Beckwith, J. R.: Restoration of operon activity by suppressors. In: Erwin-Baur-Gedächtnisvorlesung, Bd III, 1963: Struktur und Funktion des genetischen Materials, p. 119–124. Berlin: Akademie-Verlag 1964.
—: A deletion analysis of the lac operator region inE. coli. J. molec. Biol.8, 427–430 (1964).
Brenner, S., andA. O. W. Stretton: The amber-mutation. J. cell. comp. Physiol.64, Suppl. 1, 43–50 (1964).
—— andS. Kaplan: Genetic code: The “nonsense” triplets for chain termination and their suppression. Nature (Lond.)206, 994–998 (1965).
Epstein, R. H., A. Bolle, C. M. Steinberg, E. Kellenberger, E. Boy de la Tour, R. Chevalley, R. S. Edgar, M. Susman, G. H. Denhardt, andA. Lielausius: Physiological studies of conditional lethal mutants of bacteriophage T4D. Cold Spr. Harb. Symp. quant. Biol.28, 375–394 (1963).
Henning, U., G. Dennert, K. Szolyvay, andG. Deppe: J. molec. Biol. (im Druck).
—, u.C. Herz: Ein Strukturgen-Komplex für den Pyruvatdehydrogenase-Komplex vonE. coliK12. Z. Vererbungsl.95, 260–275 (1964).
—— u.K. Szolyvay: Polarisation und Disproportionalität der Synthese von Enzymkomponenten des Pyruvatdehydrogenase-Komplexes als Mutationsfolge inE. coli K12. Z. Vererbungsl.95, 236–259 (1964).
—,K. Szolyvay u.C. Herz: Der Einfluß von Suppressor-Gen-Mutation und intragenischer Reversion auf Mutationsorte vom 0°-Typ. Z. Vererbungsl.95, 276–287 (1964).
Hirsch-Kauffmann, M.: Diss. Universität Köln (in Vorbereitung).
Jacob, F., andJ. Monod: On the regulation of gene activity. Cold Spr. Harb. Symp. quant. Biol.26, 193–209 (1961).
——: Genetic mapping of the elements of the lactose region inEscherichia coli. Biochem. biophys. Res. Commun.18, 693–701 (1965).
—,A. Ullman etJ. Monod: Le promoteur, élément génétique nécessaire à l'expression d'un opéron. C. R. Acad. Sci. (Paris)258, 3125–3128 (1964).
Koike, M., L. J. Reed, andW. R. Carroll:α-Keto acid dehydrogenation complexes. IV. Resolution and reconstitution of theE. coli pyruvate dehydrogenation complex. J. biol. Chem.238, 30–39 (1963).
Matsushiro, A., S. Kida, J. Ito, K. Sato, andF. Imamoto: The regulation mechanism of enzyme synthesis in tryptophan biosynthetic pathway ofEscherichia coli K12. Biochem. biophys. Res. Commun.9, 204–207 (1962).
—,K. Sato, J. Ito, S. Kida, andF. Imamoto: On the transcription of the tryptophan operon inEscherichia coli I. The tryptophan operator. J. molec. Biol.11, 54–63 (1965).
Orias, E., andT. K. Gartner: Suppression of a class of rII mutants of T4 by a suppressor of a lac-“operator negative” mutation. Proc. nat. Acad. Sci. (Wash.)52, 859–864 (1964).
Ouchterlony, O.: Diffusion-in-gel-methods for immunological analysis. Progr. Allergy5, 1–78 (1958).
Sarabhai, A. S., A. O. W. Stretton, S. Brenner, andA. Bolle: Co-linearity of the gene with the polypeptide chain. Nature (Lond.)201, 13–17 (1964).
Schwartz, N. M.: Suppression of a lac 0°-mutation inEscherichia coli. J. Bact.88, 996–1001 (1964).
Somerville, R. L., andC. Yanofsky: On the translation of the A gene region of tryptophan messenger-RNA. J. molec. Biol.8, 616–619 (1964).
——: Studies on the regulation of tryptophan biosynthesis inEscherichia coli. J. molec. Biol.11, 747–759 (1965).
Stretton, A. O. W., andS. Brenner: Molecular consequences of theamber mutation and its suppression. J. molec. Biol.12, 456–465 (1965).
Willson, C., D. Perrin, M. Cohn, F. Jacob, andJ. Monod: Non-inducible mutants of the regulator gene in the “Lactose” system ofE. coli. J. molec. Biol.8, 582–592 (1964).
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Henning, U., Dennert, G., Szolyvay, K. et al. Mutationsorte vom oo-Typ in einem Strukturgen. Zeitschrift für Vererbungslehre 97, 1–9 (1965). https://doi.org/10.1007/BF00898567
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DOI: https://doi.org/10.1007/BF00898567